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Preparation of Bacillus subtilis Cell Lysates and Membranes   

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Original research article

A brief version of this protocol appeared in:
Molecular Microbiology
Jun 2013

Abstract

A common feature of every eukaryotic and prokaryotic cell is that they exhibit a plasma membrane. In Bacillus subtilis (B. subtilis) roughly 25% of all proteins are putative trans- or membrane associated proteins. Here we describe a relatively simple method to separate and prepare membrane and cytosolic proteins by ultra-centrifugation.

Copyright: © 2014 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Bach, J. N. and Bramkamp, M. (2014). Preparation of Bacillus subtilis Cell Lysates and Membranes. Bio-protocol 4(2): e1025. DOI: 10.21769/BioProtoc.1025.
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