Abstract
Monocular deprivation is an experimental technique to study the ocular dominance plasticity during critical period (Hubel and Wiesel, 1963). Generally one eye of an animal is sutured during critical period, and the sutured eye is re-opened after either less than three days (short term) or more than three days (long term). Here we describe a detailed protocol for short-term and long-term monocular deprivation in mouse (Ma et al., 2013).
Materials and Reagents
Equipment
Procedure
Acknowledgments
This protocol is adapted from a published paper: Ma et al. (2013). This work was supported by grants to H.W.T. from the National Institutes of Health (EY019049 and EY022478) and the Kirchgessner Foundation.
References
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