Library Construction for Genome-wide Bisulfite Sequencing in Plants   

Edited by
Feng Li
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Original research article

A brief version of this protocol appeared in:
The Plant Cell
Feb 2013


DNA methylation is the most studied epigenetic modification, which involves the addition of a methyl group to the carbon-5 position of cytosine residues in DNA. DNA methylation is important for the regulation of gene expression. Bisulfite sequencing is the gold standard technique for determining genome-wide DNA methylation profiles in eukaryotes. This protocol describes how to prepare libraries of genomic DNA for whole-genome bisulfite sequencing in Arabidopsis, which could be adapted for use in other plant species.

Copyright: © 2013 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Amador, D. M., Wang, C., Holland, K. H. and Mou, Z. (2013). Library Construction for Genome-wide Bisulfite Sequencing in Plants. Bio-protocol 3(24): e1013. DOI: 10.21769/BioProtoc.1013.

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