Abstract
This protocol is optimized for immunoFISH staining of OCT section of mouse tissues. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) (Le et al., 2010) and FISH against telomeric DNA.
Keywords: ImmunoFISH, DNA damage response, Telomeres, Baboon tissues
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
The immunofluorescence part of the protocol is adapted from Le et al. (2010). The F.d’A.d.F. laboratory is supported by FIRC (Fondazione Italiana per la Ricerca sul Cancro), AIRC (Associazione Italiana per la Ricerca sul Cancro), European Union (GENINCA, contract number 202230), HFSP (Human Frontier Science Program), AICR (Association for International Cancer Research), EMBO Young Investigator Program and Telethon.
References
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