Sanjib Kumar Panda —BIO-PROTOCOL

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联合作者（）

Hiroyuki Koyama Gifu University Gifu
1 protocol

Bedabrata Saha Newe Ya'ar Reseach Centre, Agricultural Research Organization, Ramat Yishay Ramat Yishay
2 protocols

Jay Prakash Awasthi Govt. college lamta, balaghat Balaghat
2 protocols

Pankaj Borgohain Department of Life Science and Bioinformatics, Assam University, India, India,
1 protocol

Sanjib Kumar Panda

Central University of Rajasthan Ajmer

已发表 protocol 2篇
参审 protocol 篇
主审 protocol 篇

Education

DSc, Utkal University, India

Current position

Professor, Life Science and Bioinformatics, Assam University, Silchar, India

Publications

https://scholar.google.co.in/citations?user=TI27YTgAAAAJ&hl=en

已发表 protocol 2篇

Quantitative Analysis of Redox Pool (NAD⁺, NADH Content) in Plant Samples Under Aluminum Stress
铝胁迫下植物样品氧化还原池(NAD⁺， NADH含量)的定量分析

作者：Jay Prakash Awasthi, Bedabrata Saha, Hiroyuki Koyama and Sanjib Kumar Panda，日期：06/20/2022，浏览量：1258，Q&A： 0

Nicotinamide adenine dinucleotide (NAD) is an essential cofactor of numerous enzymatic reactions found in all living cells. Pyridine nucleotides (NAD+ and NADH) are also key players in signaling through reactive oxygen species (ROS), being crucial

...

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Qualitative Analysis of Lipid Peroxidation in Plants under Multiple Stress Through Schiff’s Reagent: A Histochemical Approach
植物多重应激下脂质过氧化作用的希夫试剂定性分析：组织化学方法

作者：Jay Prakash Awasthi, Bedabrata Saha, Bhaben Chowardhara, Sanjenbam Sanjibia Devi, Pankaj Borgohain and Sanjib Kumar Panda，日期：04/20/2018，浏览量：12102，Q&A： 3

Lipid peroxidation is a physiological indicator of both biotic and abiotic stress responses, hence is often used as a biomarker to assess stress-induced cell damage or death. Here we demonstrate an easy, quick and cheap staining method to assess ...

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TINA AGARWAL

PURDUE UNIVERSITY

Has anyone tried this method with leaf tissue, can the whole leaf be stained or do we need to make section or leaf disc is needed for staining any suggestion?

5/18/2021 11:20:03 AM 回复

TINA AGARWAL

PURDUE UNIVERSITY

Thanks Bedabrata, I did take a look at your paper, so yeah! thank you for sharing that, and will definitely get back to you in case I have any further questions.

2021-05-21 08:14:41

TINA AGARWAL

PURDUE UNIVERSITY

Thank you!

2021-05-21 08:08:37

Bedabrata Saha

Newe Ya'ar Reseach Centre, Agricultural Research Organization, Ramat Yishay Ramat Yishay

Hi Tina,
Thank you for asking. We ourselves have used this protocol for rice leaf tissue segments.
Sahoo, S., Saha, B., Awasthi, J. P., Omisun, T., Borgohain, P., Hussain, S., ... & Panda, S. K. (2019). Physiological introspection into differential drought tolerance in rice cultivars of North East India. Acta Physiologiae Plantarum, 41(4), 1-15.
Besides whole leaf can also be used for staining. It just depends on how you want to represent. If you are facing any problem while performing the experiment feel free to contact us again.

2021-05-19 23:46:55

Jay Awasthi

Govt. college lamta, balaghat Balaghat

We can take whole leaf or small leaf section for this experiment. Thanks for your questions.

2021-05-19 23:43:28