编辑推荐
Editors' Choice corresponds to recently published bio-protocols (past one year) that chosen by Bio-protocol Associate Editors based on the protocols' quality, novelty, or potential impact.
研究领域
  • 生物化学
  • 癌症生物学
  • 细胞生物学
  • 发育生物学
  • 免疫学
  • 微生物学
  • 分子生物学
  • 神经科学
  • 植物科学
  • 干细胞
  • 系统生物学
研究物种
  • 节肢动物
  • 鱼类
  • 真菌
  • 哺乳纲
  • 其它脊椎动物
  • 植物
  • 原核生物
  • 原生动物
  • 病毒
  • 蠕虫
Accurate, Streamlined Analysis of mRNA Translation by Sucrose Gradient Fractionation
通过蔗糖梯度分级分离准确、高效分析mRNA的翻译
作者:Soufiane Aboulhouda, Rachael Di Santo, Gabriel Therizols and David Weinberg日期:10/05/2017,浏览量:3525,Q&A: 0
The efficiency with which proteins are produced from mRNA molecules can vary widely across transcripts, cell types, and cellular states. Methods that accurately assay the translational efficiency of ...
Large-scale Maize Seedling Infection with Exserohilum turcicum in the Greenhouse
温室中大规模玉米幼苗感染玉米大斑病菌
作者:Ping Yang, Gerhard Herren, Simon G. Krattinger and Beat Keller日期:10/05/2017,浏览量:3029,Q&A: 0
Northern corn leaf blight (NCLB) is a serious foliar disease of maize (Zea mays) worldwide and breeding for resistance is of primary importance for maize crop protection. Phenotyping for ...
Peroxisome Motility Measurement and Quantification Assay
过氧化物酶体能动性测量和定量分析
作者:Jeremy Metz, Inês G. Castro and Michael Schrader日期:09/05/2017,浏览量:2377,Q&A: 0
Organelle movement, distribution and interaction contribute to the organisation of the eukaryotic cell. Peroxisomes are multifunctional organelles which contribute to cellular lipid metabolism and ...
An ex vivo Perifusion Method for Quantitative Determination of Neuropeptide Release from Mouse Hypothalamic Explants
用于定量测定小鼠下丘脑外植体神经肽释放的离体灌流方法
作者:Ophélia Le Thuc, Jacques Noël and Carole Rovère日期:08/20/2017,浏览量:2683,Q&A: 0
The hypothalamus is a primary brain area which, in mammals, regulates several physiological functions that are all related to maintaining general homeostasis, by linking the central nervous system ...
Protocol for HeLa Cells Infection with Escherichia coli Strains Producing Colibactin and Quantification of the Induced DNA-damage
用可产生大肠杆菌毒素的大肠埃希杆菌菌株感染HeLa细胞的实验方案及诱导产生DNA损伤的定量
作者:Nadège Bossuet-Greif, Marcy Belloy, Michèle Boury, Eric Oswald and Jean-Philippe Nougayrede日期:08/20/2017,浏览量:3651,Q&A: 0
Strains of Escherichia coli bearing the pks genomic island synthesize the genotoxin colibactin. Exposure of eukaryotic cells to E. coli producing colibactin induces DNA ...
Liposome Disruption Assay to Examine Lytic Properties of Biomolecules
采用脂质体破碎测定法检测生物分子的裂解性质
作者:John R. Jimah, Paul H. Schlesinger and Niraj H. Tolia日期:08/05/2017,浏览量:4178,Q&A: 0
Proteins may have three dimensional structural or amino acid features that suggest a role in targeting and disrupting lipids within cell membranes. It is often necessary to experimentally investigate ...
Membrane Lipid Screen to Identify Molecular Targets of Biomolecules
采用膜脂筛查法鉴定生物分子的分子靶点
作者:John R. Jimah, Paul H. Schlesinger and Niraj H. Tolia日期:08/05/2017,浏览量:3040,Q&A: 0
Proteins that bind to and disrupt cell membranes may target specific phospholipids. Here we describe a protocol to identify the lipid targets of proteins and biomolecules. First, we describe a screen ...
Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters
肠杆菌科中受阿拉伯糖调节启动子调控的基因剂量实验
作者:Sanchari Bhattacharyya, Shimon Bershtein and Eugene I Shakhnovich日期:07/20/2017,浏览量:2438,Q&A: 0
This protocol is used to assay the effect of protein over-expression on fitness of E. coli. It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD ...
Spinal Cord Preparation from Adult Red-eared Turtles for Electrophysiological Recordings during Motor Activity
制备成年红耳龟脊髓以用于运动活动期间电生理的记录
作者:Peter C Petersen and Rune W Berg 日期:07/05/2017,浏览量:4259,Q&A: 0
Although it is known that the generation of movements is performed to a large extent in neuronal circuits located in the spinal cord, the involved mechanisms are still unclear. The turtle as a model ...
Single-molecule RNA Fluorescence in situ Hybridization (smFISH) in Caenorhabditis elegans
秀丽隐杆线虫中的单分子RNA荧光原位杂交(smFISH)
作者:ChangHwan Lee, Hannah S. Seidel, Tina R. Lynch, Erika B. Sorensen, Sarah L. Crittenden and Judith Kimble日期:06/20/2017,浏览量:5949,Q&A: 0
Single-molecule RNA fluorescence in situ hybridization (smFISH) is a technique to visualize individual RNA molecules using multiple fluorescently-labeled oligonucleotide probes specific to ...