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Editors' Choice corresponds to recently published bio-protocols (past one year) that chosen by Bio-protocol Associate Editors based on the protocols' quality, novelty, or potential impact.
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Peptide Feeding and Mechanical Wounding for Tomato Seedlings
番茄幼苗的肽饲喂和机械伤害
作者:
Lei Wang
,
Yan Wang
and
Georg Felix
,
日期:
03/20/2019,
浏览量:
4878,
Q&A:
0
Plants need to respond appropriately to wounding and herbivorous insects. Peptide signals have been implicated in local and systemic induction of appropriate plant defense responses. To study these ...
Viral Chromosome Conformation Capture (V3C) Assays for Identifying Trans-interaction Sites between Lytic Viruses and the Cellular Genome
病毒染色体构象俘获技术用于裂解病毒和细胞基因组之间反向相互作用位点的检测
作者:
Kinjal Majumder
,
Maria Boftsi
and
David J Pintel
,
日期:
03/20/2019,
浏览量:
4013,
Q&A:
0
The folding mechanisms of the mammalian genome package our genetic material into the nucleus, and in doing so, dictate its appropriate replication and expression. Chromosome conformation capture ...
Straight Channel Microfluidic Chips for the Study of Platelet Adhesion under Flow
直通道微流控芯片用于研究流动中的血小板黏附
作者:
Alexander Dupuy
,
Lining Arnold Ju
and
Freda H Passam
,
日期:
03/20/2019,
浏览量:
12129,
Q&A:
0
Microfluidic devices have become an integral method of cardiovascular research as they enable the study of shear force in biological processes, such as platelet function and thrombus formation. ...
Detection of mRNA by Whole Mount
in situ
Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
整体原位杂交检测mRNA并提取DNA检测斑马鱼胚胎的基因分型
作者:
Rachna Narayanan
and
Andrew C. Oates
,
日期:
03/20/2019,
浏览量:
12943,
Q&A:
0
In situ
hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current ...
Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) into an Effective Model of Forebrain Neural Progenitor Cells and Mature Neurons
人诱导多能干细胞 (iPSCs) 分化为前脑神经祖细胞和成熟神经元的有效模型
作者:
Scott Bell
,
Nuwan C. Hettige
,
Heika Silveira
,
Huashan Peng
,
Hanrong Wu
,
Malvin Jefri
,
Lilit Antonyan
,
Ying Zhang
,
Xin Zhang
and
Carl Ernst
,
日期:
03/05/2019,
浏览量:
14380,
Q&A:
0
Induced Pluripotent Stem Cells (iPSCs) are pluripotent stem cells that can be generated from somatic cells, and provide a way to model the development of neural tissues
in vitro
. One ...
Isolation of Neural Stem Cells from the Embryonic Mouse Hippocampus for
in vitro
Growth or Engraftment into a Host Tissue
从胚胎小鼠海马分离神经干细胞用于体外生长或植入宿主组织
作者:
Oksana Rybachuk
,
Olga Kopach
,
Tetyana Pivneva
and
Vitaliy Kyryk
,
日期:
02/20/2019,
浏览量:
7889,
Q&A:
0
For both stem cell research and treatment of the central nervous system disorders, neural stem/progenitor cells (NSPCs) represent an important breakthrough tool. In the expanded stem cell-based ...
Imaging Higher-order Chromatin Structures in Single Cells Using Stochastic Optical Reconstruction Microscopy
随机光学重建显微镜用于单细胞中高级染色体结构的成像
作者:
Jianquan Xu
and
Yang Liu
,
日期:
02/05/2019,
浏览量:
6398,
Q&A:
0
Higher-order chromatin organization shaped by epigenetic modifications influence the chromatin environment and subsequently regulate gene expression. Direct visualization of the higher-order ...
In situ
, Cell-free Protein Expression on Microarrays and Their Use for the Detection of Immune Responses
基于原位无细胞蛋白表达的蛋白质微阵列技术及其在免疫应答检测中的应用
作者:
Katrin Hufnagel
,
Dennis Reininger
,
Siu Wang Ng
,
Nadine Gassert
,
Juliane K. Rohland
,
Soroosh Shahryarhesami
,
Andrea S. Bauer
,
Tim Waterboer
and
Jörg D. Hoheisel
,
日期:
02/05/2019,
浏览量:
5047,
Q&A:
0
Until recently, whole-proteome microarrays for comprehensive studies of protein interactions were mostly produced by individual cloning and cellular expression of very many open reading frames, ...
Measuring Homologous Recombination Rates between Chromosomal Locations in
Salmonella
沙门氏菌中染色体位点间的同源重组率测定
作者:
Gerrit Brandis
,
Sha Cao
and
Diarmaid Hughes
,
日期:
02/05/2019,
浏览量:
6571,
Q&A:
0
Homologous recombination between two similar DNA molecules, plays an important role in the repair of double-stranded DNA breaks. Recombination can occur between two sister chromosomes, or between two ...
CRISPR-Cas9 Mediated Genome Editing in
Drosophila
果蝇中CRISPR-Cas9介导的基因组编辑
作者:
Ping Peng
,
Xia Wang
,
Da Shen
,
Jin Sun
,
Yu Jia
,
Rong-Gang Xu
,
Li-Fei Zhu
and
Jian-Quan Ni
,
日期:
01/20/2019,
浏览量:
9284,
Q&A:
0
In recent years, great progress has been made in the research of genome editing systems, one of which is the CRISPR-Cas9 system, a powerful technology that is applied to edit animal genome. Here, we ...