植物科学

分类

    现刊
    An Image Analysis Pipeline to Quantify Emerging Cracks in Materials or Adhesion Defects in Living Tissues
    分析活体组织中出现的材料裂缝或附着力缺陷的图像分析法
    作者:Stéphane Verger, Guillaume Cerutti and Olivier Hamant日期:10/05/2018,浏览量:5191,Q&A: 0
    [Abstract] Microcracks in materials reflect their mechanical properties. The quantification of the number or orientation of such cracks is thus essential in many fields, including engineering and geology. In biology, cracks in soft tissues can reflect adhesion defects, and the analysis of their pattern can help to deduce the magnitude and orientation of ...
    Using Silicon Polymer Impression Technique and Scanning Electron Microscopy to Measure Stomatal Aperture, Morphology, and Density
    使用硅聚合物印模技术和扫描电子显微镜技术测量气孔的开度、形态和密度
    作者:Hui-Chen Wu, Ya-Chen Huang, Chia-Hung Liu and Tsung-Luo Jinn日期:08/20/2017,浏览量:9039,Q&A: 0
    [Abstract] The number of stomata on leaves can be affected by intrinsic development programming and various environmental factors, in addition the control of stomatal apertures is extremely important for the plant stress response. In response to elevated temperatures, transpiration occurs through the stomatal apertures, allowing the leaf to cool through ...
    Root Aliphatic Suberin Analysis Using Non-extraction or Solvent-extraction Methods
    不萃取或溶剂萃取法分析根脂肪族木栓质
    作者:Camille Delude, Sollapura J. Vishwanath, Owen Rowland and Frédéric Domergue日期:06/20/2017,浏览量:9249,Q&A: 1
    [Abstract] Here we describe both non-extraction and solvent-extraction methods for root aliphatic suberin analysis. The non-extraction method is fast as roots are directly depolymerized using acidic transmethylation. However, suberin aliphatic components are isolated together with all the other acyl chains making up the lipids (e.g., membranes) ...
    Determining Genome Size from Spores of Seedless Vascular Plants
    无种子维管束植物孢子基因组大小的测定
    作者:Li-Yaung Kuo and Yao-Moan Huang 日期:06/05/2017,浏览量:7835,Q&A: 0
    [Abstract] Seedless vascular plants, including ferns and lycophytes, produce spores to initiate the gametophyte stage and to complete sexual reproduction. Approximately 10% of them are apomictic through the production of genomic unreduced spores. Being able to measure the spore nuclear DNA content is therefore important to infer their reproduction mode. Here ...
    Documentation of Floral Secretory Glands in Pleurothallidinae (Orchidaceae) Using Scanning Electron Microscopy (SEM)
    使用扫描电子显微镜(SEM)观察肋茎兰(兰科)花分泌腺
    作者:Adam P. Karremans, Bertie Joan van Heuven, Rob Langelaan and Barbara Gravendeel日期:11/20/2016,浏览量:9142,Q&A: 0
    [Abstract] A clear, step by step description of the treatment of orchid flowers, subtribe Pleurothallidinae, with Critical Point Drying for SEM is presented. It shows that a simple, short fixation and dehydration method prior to Critical Point Drying is sufficient to obtain good results.
    Cell Wall-bound p-Coumaric and Ferulic Acid Analysis
    细胞壁结合的p-香豆和阿魏酸的分析
    作者:Nickolas Anderson日期:10/05/2016,浏览量:7151,Q&A: 0
    [Abstract] Hydroxycinnamic acids, such as p-coumaric acid and ferulic acid, are a major class of compounds derived from the phenylpropanoid pathway. These compounds are widely conserved in plants and primarily accumulate in the secondary cell wall. They serve as important structural components that contribute to the overall strength and rigidity of ...
    Sample Preparation for X-ray Micro-computed Tomography of Woody Plant Material and Associated Xylem Visualisation Techniques
    木本植物材料样本的制备用于X射线显微计算机断层扫描和木质部可视化技术
    作者:Nicholas J. B. Brereton日期:03/20/2016,浏览量:9137,Q&A: 0
    [Abstract] Variation in the tissue structure of short rotation coppice (SRC) willow is a principle factor driving differences in lignocellulosic sugar yield yet much of the physiology and development of this tissue is unknown. Traditional sectioning can be both difficult and destructive in woody tissue; however, technology such as three dimensional X-ray ...
    Extraction of Intracellular and Cell Wall Proteins from Leaves and Roots of Harsh Hakea
    从粗糙哈克木属植物叶和根中提取胞内和细胞壁蛋白质
    作者:Michael W. Shane and William C. Plaxton日期:12/05/2015,浏览量:8700,Q&A: 0
    [Abstract] Plant proteins can be targeted to intracellular (i.e., cytosol, vacuole, organelles etc.) or extracellular (i.e., cell walls, apoplast) compartments. Dual targeting is a key mechanism with important implications for plant metabolism, growth, development and defense etc. Harsh Hakea (Hakea prostrata ...
    Detection and Visualization of Specific Gene Transcripts by in situ RT-PCR in Nematode-Infected Arabidopsis Root Tissue
    采用原位RT-PCR法检测和显示线虫感染的拟南芥根部组织中特定基因的转录水平
    作者:Krzysztof Wieczorek日期:09/20/2015,浏览量:9213,Q&A: 0
    [Abstract] This protocol describes an effective method of in situ RT-PCR that was developed to localize specific gene expression directly in thin cross-sections of nematode feeding sites induced by the cyst nematode Heterodera schachtii (H. schachtii) or the root-knot nematode Meloidogyne incognita (M. incognita) ...
    Transverse Sectioning of Arabidopsis thaliana Leaves Using Resin Embedding
    树脂包埋制备拟南芥横切片
    作者:Shweta Kalve, Kumud Saini, Kris Vissenberg, Tom Beeckman and Gerrit T. S. Beemster日期:09/20/2015,浏览量:12628,Q&A: 0
    [Abstract] The leaf is the major functional part of the shoot performing the bulk of photosynthetic activity. Its development is relatively plastic allowing the plant to adapt to environmental changes by modifying leaf size and anatomy. Moreover, a leaf is made up of various distinct cell layers, each having specialized functions. To understand functional ...