微生物学

分类

    现刊
    In vitro Di-ubiquitin Formation Assay and E3 Cooperation Assay
    体外双泛素形成检测方法和E3协同分析方法
    作者:Rebecca J. Burge, Katie H. Jameson, Anthony J. Wilkinson and Jeremy C. Mottram日期:11/05/2022,浏览量:491,Q&A: 0
    [Abstract]

    Ubiquitination is a post-translational modification conserved across eukaryotic species. It contributes to a variety of regulatory pathways, including proteasomal degradation, DNA repair, and cellular differentiation. The ubiquitination of substrate proteins typically requires three ubiquitination enzymes: a ubiquitin-activating E1, a

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    Negative Staining Transmission Electron Microscopy of HIV Viral Particles Permeabilized with PFO and Capsid Stabilized with IP6
    应用透射电子显微镜负染技术观察 PFO 透化的和 IP6 稳定衣壳的 HIV 病毒颗粒
    作者:Derrick Lau, Chantal L. Márquez, Michael W. Parker and Till Böcking日期:10/20/2022,浏览量:624,Q&A: 0
    [Abstract]

    The human immunodeficiency virus 1 (HIV-1) consists of a viral membrane surrounding the conical capsid. The capsid is a protein container assembled from approximately 1,500 copies of the viral capsid protein (CA), functioning as a reaction and transport chamber for the viral genome after cell entry. Transmission electron microscopy (TEM) is a

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    Assay for Protealysin-like Protease Inhibitor Activity
    蛋白酶样蛋白酶抑制剂活性测定
    作者:Igor M. Berdyshev, Maria A. Karaseva and Ilya V. Demidyuk日期:10/05/2022,浏览量:537,Q&A: 0
    [Abstract]

    Here, we present the first quantitative method for the activity analysis of protealysin-like protease (PLP) inhibitors. This approach is based on a previously developed method for protealysin activity determination by hydrolysis of internally quenched fluorescent peptide substrate

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    Analysis of Lipid-linked Oligosaccharides Synthesized in vivo in Schizosaccharomyces pombe
    裂殖酵母体内合成的脂联寡糖分析
    作者:Ayelen Valko, Giovanna L. Gallo, Ariel D. Weisz, Armando J. Parodi and Cecilia D’Alessio日期:09/20/2022,浏览量:591,Q&A: 0
    [Abstract]

    Dolichol diphosphate-linked oligosaccharides (LLO) are the sugar donors in N-glycosylation, a fundamental protein post-translational modification of the eukaryotic secretory pathway. Defects in LLO biosynthesis produce human Congenital Disorders of Glycosylation Type I. The synthesis of LLOs and the transfer reactions to their protein

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    Split-Chloramphenicol Acetyl Transferase Assay to Study Protein-Protein Interactions and Ubiquitylation in Escherichia coli
    分裂氯霉素乙酰转移酶测定法研究大肠杆菌中的蛋白质-蛋白质相互作用和泛素化
    作者:Amir Florentin, Alina Kordonsky, Elon Yariv, Reut Avishid, Noa Efron, Edache Akogwu and Gali Prag日期:09/05/2022,浏览量:756,Q&A: 0
    [Abstract]

    Protein-protein interactions and protein modifications play central roles in all living organisms. Of the more than 200 types of post-translational modifications, ubiquitylation is the most abundant, and it profoundly regulates the functionality of the eukaryotic proteome. Various in vitro and in vivo methodologies to study protein interactions

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    Binding Affinity Quantifications of the Bacteriophage Mu DNA Modification Protein Mom Using Microscale Thermophoresis (MST)
    使用微尺度热泳 (MST) 对噬菌体 Mu DNA 修饰蛋白Mom的结合亲和力定量
    作者:Shubha Udupa, Valakunja Nagaraja and Shweta Karambelkar日期:07/20/2022,浏览量:1523,Q&A: 0
    [Abstract]

    Epigenetic modifications play diverse roles in biological systems. Nucleic acid modifications control gene expression, protein synthesis, and sensitivity to nucleic acid-cleaving enzymes. However, the mechanisms underlying the biosynthesis of nucleic acid modifications can be challenging to identify. Studying protein-ligand interactions helps

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    Tracking the Reversed Oxidative Tricarboxylic Acid Cycle in Bacteria
    追踪细菌中的反向氧化三羧酸循环
    作者:Lydia Steffens, Eugenio Pettinato, Thomas M. Steiner, Wolfgang Eisenreich and Ivan A. Berg日期:03/20/2022,浏览量:1533,Q&A: 0
    [Abstract]

    Different pathways for autotrophic CO2 fixation can be recognized by the presence of genes for their specific key enzymes. On this basis, (meta)genomic, (meta)transcriptomic, or (meta)proteomic analysis enables the identification of the role of an organism or a distinct pathway in primary production. However, the recently discovered variant of the

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    Activity-based Crosslinking to Identify Substrates of Thioredoxin-domain Proteins in Malaria Parasites
    基于活性的交联识别疟原虫中硫氧还蛋白结构域蛋白的底物
    作者:David W. Cobb, Grace S. Woods and Vasant Muralidharan日期:02/20/2022,浏览量:1581,Q&A: 0
    [Abstract] Malaria remains a major public health issue, infecting nearly 220 million people every year. The spread of drug-resistant strains of Plasmodium falciparum around the world threatens the progress made against this disease. Therefore, identifying druggable and essential pathways in P. falciparum parasites remains a major area of ...
    Isolation of Mitochondria from Ustilago maydis Protoplasts
    从黑粉病原生质体中分离线粒体
    作者:Juan Pablo Pardo, Guadalupe Guerra-Sánchez, Oscar Flores-Herrera and Lucero Romero-Aguilar日期:01/05/2022,浏览量:1337,Q&A: 0
    [Abstract]

    Ustilago maydis, a basidiomycete that infects Zea mays, is one of the top ten fungal models for studying DNA repair, signal transduction pathways, and dimorphic transitions, among other processes. From a metabolic point of view, U. maydis lacks fermentative capacity, pointing to mitochondria as a key player in central metabolism. Oxidative

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    Extraction and Electrophoretic Analysis of Bacterial Lipopolysaccharides and Outer Membrane Proteins
    细菌脂多糖和外膜蛋白的提取和电泳分析
    作者:Yue-Jia Lee and Thomas J. Inzana日期:12/20/2021,浏览量:2336,Q&A: 0
    [Abstract]

    Lipopolysaccharides (LPS) (or lipooligosaccharides [LOS], which lack the O-antigen side chains characteristic of LPS), and outer membrane proteins (OMP) are major cell-surface molecules in the outer membrane (OM) of gram-negative bacteria. The LPS is responsible for causing endotoxic shock in infected hosts and, in conjunction with some OMPs,

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