发育生物学

分类

    现刊
    Generation of iMyoblasts from Human Induced Pluripotent Stem Cells
    从人诱导的多能干细胞产生成肌细胞
    作者:Dongsheng Guo, Katelyn Daman, Danielle Fernandes Durso, Jing Yan and Charles P. Emerson Jr.日期:09/05/2022,浏览量:934,Q&A: 0
    [Abstract]

    Skeletal muscle stem cells differentiated from human-induced pluripotent stem cells (hiPSCs) serve as a uniquely promising model system for investigating human myogenesis and disease pathogenesis, and for the development of gene editing and regenerative stem cell therapies. Here, we present an effective and reproducible transgene-free protocol for

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    Fluorescence Imaging of 3D Cell Models with Subcellular Resolution
    具有亚细胞分辨率的 3D 细胞模型的荧光成像
    作者:Indra Van Zundert, Nina Maenhoudt, Silke De Vriendt, Hugo Vankelecom, Beatrice Fortuni and Susana Rocha日期:07/20/2022,浏览量:2292,Q&A: 0
    [Abstract]

    Over the past years, research has made impressive breakthroughs towards the development and implementation of 3D cell models for a wide range of applications, such as drug development and testing, organogenesis, cancer biology, and personalized medicine. Opposed to 2D cell monolayer culture systems, advanced 3D cell models better represent the in

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    ATAC-Seq of a Single Myofiber from Mus musculus
    小家鼠单一肌纤维的ATAC-Seq
    作者:Korin Sahinyan, Darren M. Blackburn and Vahab D. Soleimani日期:06/20/2022,浏览量:2059,Q&A: 0
    [Abstract]

    Chromatin accessibility is a key determinant of gene expression that can be altered under different physiological and disease conditions. Skeletal muscle is made up of myofibers that are highly plastic and adaptive. Therefore, assessing the genome-wide chromatin state of myofibers under various conditions is very important to gain insight into the

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    Labelling of Active Transcription Sites with Argonaute NRDE-3—Image Active Transcription Sites in vivo in Caenorhabditis elegans
    用Argonaute NRDE-3标记活性转录位点—图像秀丽隐杆线虫体内的活性转录位点
    作者:Antoine Barrière and Vincent Bertrand日期:06/05/2022,浏览量:1053,Q&A: 0
    [Abstract]

    Live labelling of active transcription sites is critical to our understanding of transcriptional dynamics. In the most widely used method, RNA sequence MS2 repeats are added to the transcript of interest, on which fluorescently tagged Major Coat Protein binds, and labels transcription sites and transcripts. Here we describe another strategy, using

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    Simple Methods for Permanent or Transient Denervation in Mouse Sciatic Nerve Injury Models
    小鼠坐骨神经损伤模型中永久性或暂时性去神经的简单方法
    作者:Alexis Osseni, Jean-Luc Thomas, Alireza Ghasemizadeh, Laurent Schaeffer and Vincent Gache日期:06/05/2022,浏览量:1261,Q&A: 0
    [Abstract]

    Our ability to move and breathe requires an efficient communication between nerve and muscle that mainly takes place at the neuromuscular junctions (NMJs), a highly specialized synapse that links the axon of a motor neuron to a muscle fiber. When NMJs or axons are disrupted, the control of muscle fiber contraction is lost and muscle are paralyzed.

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    Isolation of tdTomato Expressing Inter-follicular Epidermal Melanocytes or Keratinocytes from Mouse Tail Skin
    从小鼠尾部皮肤中分离tdTomato表达滤泡间表皮黑色素细胞或角质形成细胞
    作者:Samuel Pop, Oscar Urtatiz and Catherine D. Van Raamsdonk日期:04/20/2022,浏览量:1771,Q&A: 0
    [Abstract]

    The epidermis is the outermost layer of the skin. It is made up of mostly keratinocytes along with a small number of melanocytes and Langerhans cells. Melanocytes produce a pigment called melanin, which is transferred to the keratinocytes, and protects these cells from damage from UV radiation, as well as generating hair and skin colours. In this

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    Isolation and Culture of Cranial Neural Crest Cells from the First Branchial Arch of Mice
    小鼠第一鳃弓颅神经嵴细胞的分离培养
    作者:Hiroki Ueharu, Jingwen Yang, Yoshihiro Komatsu and Yuji Mishina日期:04/05/2022,浏览量:1267,Q&A: 1
    [Abstract]

    Craniofacial anomalies (CFA) are a diverse group of deformities, which affect the growth of the head and face. Dysregulation of cranial neural crest cell (NCC) migration, proliferation, differentiation, and/or cell fate specification have been reported to contribute to CFA. Understanding of the mechanisms through which cranial NCCs contribute

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    Generation, Maintenance and HBV Infection of Human Liver Organoids
    人肝类器官的产生、维持和HBV感染
    作者:Shahla Romal, Tanvir Hossain and Tokameh Mahmoudi日期:03/20/2022,浏览量:1757,Q&A: 0
    [Abstract]

    Hepatitis B virus (HBV) infection represents a major public health problem infecting approximately 400 million people worldwide. Despite the availability of a preventive vaccine and anti-viral therapies, chronic HBV infection remains a major health issue because it increases the risk of developing liver cirrhosis and hepatocellular carcinoma

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    3D-Structured Illumination Microscopy of Centrosomes in Human Cell Lines
    人类细胞系中心体的 3D 结构照明显微镜
    作者:Kari-Anne M. Frikstad, Kay O. Schink, Sania Gilani, Lotte B. Pedersen and Sebastian Patzke日期:03/20/2022,浏览量:1507,Q&A: 0
    [Abstract]

    The centrosome is the main microtubule-organizing center of animal cells, and is composed of two barrel-shaped microtubule-based centrioles embedded in protein dense pericentriolar material. Compositional and architectural re-organization of the centrosome drives its duplication, and enables its microtubule-organizing activity and capability to

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    NBD-lipid Uptake Assay for Mammalian Cell Lines
    哺乳动物细胞系的 NBD 脂质摄取测定
    作者:Sara Abad Herrera, Marta Grifell-Junyent and Thomas Günther Pomorski日期:02/20/2022,浏览量:2281,Q&A: 0
    [Abstract]

    All eukaryotic cells are equipped with transmembrane lipid transporters, which are key players in membrane lipid asymmetry, vesicular trafficking, and membrane fusion. The link between mutations in these transporters and disease in humans highlights their essential role in cell homeostasis. Yet, many key features of their activities, their

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