免疫学

分类

    现刊
    Murine Double Hit Model for Neonatal Cardiopulmonary Diseases: Bronchopulmonary Dysplasia (BPD) and Pulmonary Hypertension Associated with BPD
    新生儿心肺疾病的小鼠双击模型:支气管肺发育不良(BPD)和与BPD相关的肺动脉高压
    作者:Steven P. Garrick, Philip J. Berger, Marcel F. Nold and Claudia A. Nold-Petry日期:11/05/2022,浏览量:451,Q&A: 0
    [Abstract]

    Bronchopulmonary dysplasia (BPD) and pulmonary hypertension associated with BPD (BPD-PH) are of multifactorial origin and share common risk factors. Most murine models of BPD expose newborn pups to only one of these risk factors—more commonly postnatal hyperoxia—thereby mimicking the vital increased fraction of inspired oxygen (FiO2)

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    Combination of Sterile Injury and Microbial Contamination to Model Post-surgical Peritoneal Adhesions in Mice
    结合无菌损伤和微生物污染对小鼠术后腹膜粘连进行建模
    作者:Julia Bayer, Deborah Stroka, Paul Kubes, Daniel Candinas and Joel Zindel日期:08/20/2022,浏览量:1239,Q&A: 0
    [Abstract]

    Abdominal surgeries are frequently associated with the development of post-surgical adhesions. These are irreversible fibrotic scar bands that appear between abdominal organs and the abdominal wall. Patients suffering from adhesions are at risk of severe complications, such as small bowel obstruction, chronic pelvic pain, or infertility. To date,

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    Cost Effective Method for gDNA Isolation from the Cecal Content and High Yield Procedure for RNA Isolation from the Colonic Tissue of Mice
    从小鼠盲肠内容物中分离gDNA的经济有效方法和从小鼠结肠组织中分离RNA的高产率方法
    作者:Sohini Mukhopadhyay and Palok Aich日期:08/05/2022,浏览量:674,Q&A: 0
    [Abstract]

    Microbiome studies are quickly gaining momentum. Since most of the resident microbes (consisting of bacteria, fungi, and viruses) are difficult to culture, sequencing the microbial genome is the method of choice to characterize them. It is therefore important to have efficient methodology for gDNA isolation of gut microbes. Mouse models are widely

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    Immunohistochemistry of Immune Cells and Cells Bound to in vivo Administered Antibodies in Liver, Lung, Pancreas, and Colon of B6/lpr Mice
    B6/lpr小鼠肝脏、肺、胰腺和结肠中免疫细胞和与体内施用的抗体结合的细胞的免疫组织化学
    作者:Kieran Adam and Adam Mor日期:07/20/2022,浏览量:1417,Q&A: 0
    [Abstract]

    Employing a novel mouse model of immune related adverse events (irAEs) induced by combination of anti-PD1 and anti-CTLA-4 antibodies, we visualized immune infiltration into the liver, lung, pancreas, and colon. Here, we describe the avidin-biotin conjugate (ABC) method used to stain T cells (CD4 and CD8), B cells (CD19), macrophages (F4/80), and

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    Assessing the Presence of Hematopoietic Stem and Progenitor Cells in Mouse Spleen
    评估小鼠脾脏中造血干细胞和祖细胞的存在
    作者:Isabelle J. Marié, Lara Brambilla and David E. Levy日期:06/05/2022,浏览量:1367,Q&A: 0
    [Abstract]

    Transplantation of hematopoietic material into recipient mice is an assay routinely used to determine the presence and function of hematopoietic stem and progenitor cells (HSPCs) in vivo. The principle of the method is to transplant donor cells being tested for HSPCs into a recipient mouse following bone marrow ablation and testing for

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    Protocol to Induce Follicular T Helper Cells, Germinal Centers, and Skin Lesions in Mouse Models for Skin Blistering Diseases
    在皮肤起泡疾病小鼠模型中诱导滤泡 T 辅助细胞、生发中心和皮肤损伤的方法
    作者:Farbod Bahreini, Markus Niebuhr, Julia Belde, Katja Bieber, Juergen Westermann and Kathrin Kalies日期:05/20/2022,浏览量:1314,Q&A: 0
    [Abstract]

    Autoreactive T cells in autoantibody-mediated autoimmune diseases can be divided into two major subsets: (i) follicular T helper cells (Tfh) that provide T cell help in germinal centers (GC) and (ii) effector T (Teff) cells that immigrate into peripheral tissue sites such as the skin and mediate local inflammation. To study the sequence of events

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    A Combinatorial in-silico, in-vitro and in-vivo Approach to Quantitatively Study Peptide Induced MHC Stability
    结合硅、体外和体内方法定量研究多肽诱导的MHC稳定性
    作者:Roman Sarkar, Yashu Sharma, Ayush Jain, Azeez Tehseen, Sudhakar Singh and Sharvan Sehrawat日期:12/20/2021,浏览量:1629,Q&A: 0
    [Abstract]

    Here, we describe a combinatorial approach in reverse vaccinology to identify immunogenic class I major histocompatibility complex (MHC) displayed epitopes derived from a morbillivirus named pestes des petits ruminants (PPRV). The protocol describes an in silico prediction of immunogenic epitopes using an IEDB tool. The predicted peptides were

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    Intact in situ Preparation of the Drosophila melanogaster Lymph Gland for a Comprehensive Analysis of Larval Hematopoiesis
    黑腹果蝇淋巴腺的完整原位制备用于综合幼虫造血分析
    作者:Diana Rodrigues, K. VijayRaghavan, Lucas Waltzer and Maneesha S. Inamdar日期:11/05/2021,浏览量:2548,Q&A: 0
    [Abstract]

    Blood cells have a limited lifespan and are replenished by a small number of hematopoietic stem and progenitor cells (HSPCs). Adult vertebrate hematopoiesis occurs in the bone marrow, liver, and spleen, rendering a comprehensive analysis of the entire HSPC pool nearly impossible. The Drosophila blood system is well studied and has developmental,

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    Isolation and Culturing Primary Cholangiocytes from Mouse Liver
    小鼠肝原代胆管细胞的分离与培养
    [Abstract]

    Cholangiocytes are epithelial cells lining the intrahepatic and extrahepatic bile ducts. Cholangiocytes perform key physiological functions in the liver. Bile synthesized by hepatocytes is secreted into bile canaliculi, further stored in the gallbladder, and finally discharged into the duodenum. Due to liver injury, biliary epithelial proliferate

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    Isolation of Nasal Brush Cells for Single-cell Preparations
    用于单细胞制备的鼻刷细胞的分离
    作者:Saltanat Ualiyeva, Amelia A. Boyd, Nora A. Barrett and Lora G. Bankova日期:09/20/2021,浏览量:2864,Q&A: 0
    [Abstract]

    Solitary chemosensory epithelial cells are scattered in most mucosal surfaces. They are referred to as tuft cells in the intestinal mucosa, brush cells in the trachea, and solitary chemosensory and microvillous cells in the nasal mucosa. They are the primary source of IL-25 in the epithelium and are also engaged in acetylcholine generation. We

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