免疫学

分类

    现刊
    Protocol to Isolate Germinal Centers by Laser Microdissection
    通过激光显微切割分离生发中心的方法
    作者:Farbod Bahreini, Markus Niebuhr, Julia Belde, Jürgen Westermann and Kathrin Kalies日期:06/05/2022,浏览量:522,Q&A: 0
    [Abstract]

    During adaptive immune responses, germinal centers (GC) appear as transient microstructures, in which antigen-specific B and T cells interact with each other. Because only the antigen-activated B and T cells, such as Plasmablasts or follicular T helper (Tfh) cells, are present in GC, the in depth-analysis of GC is of great interest. To identify

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    Flow Cytometric Characterization of Macrophages Infected in vitro with Salmonella enterica Serovar Typhimurium Expressing Red Fluorescent Protein
    表达红色荧光蛋白的肠沙门氏菌血清鼠伤寒菌体外感染巨噬细胞的流式细胞术研究
    作者:Natascha Brigo, Christa Pfeifhofer-Obermair, Egon Demetz, Piotr Tymoszuk and Günter Weiss日期:06/05/2022,浏览量:442,Q&A: 0
    [Abstract]

    Macrophages are important for host defense against intracellular pathogens like Salmonella and can be differentiated into two major subtypes. M1 macrophages, which are pro-inflammatory and induce antimicrobial immune effector mechanisms, including the expression of inducible nitric oxide synthase (iNOS), and M2 macrophages, which exert

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    Protocol to Induce Follicular T Helper Cells, Germinal Centers, and Skin Lesions in Mouse Models for Skin Blistering Diseases
    在皮肤起泡疾病小鼠模型中诱导滤泡 T 辅助细胞、生发中心和皮肤损伤的方法
    作者:Farbod Bahreini, Markus Niebuhr, Julia Belde, Katja Bieber, Juergen Westermann and Kathrin Kalies日期:05/20/2022,浏览量:865,Q&A: 0
    [Abstract]

    Autoreactive T cells in autoantibody-mediated autoimmune diseases can be divided into two major subsets: (i) follicular T helper cells (Tfh) that provide T cell help in germinal centers (GC) and (ii) effector T (Teff) cells that immigrate into peripheral tissue sites such as the skin and mediate local inflammation. To study the sequence of events

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    CD8 T Cell Virus Inhibition Assay Protocol
    CD8 T 细胞病毒抑制实验方法
    [Abstract]

    The human immunodeficiency virus (HIV)-1 viral inhibition assay (VIA) measures CD8+ T cell-mediated inhibition of HIV replication in CD4+ T cells and is increasingly used for clinical testing of HIV vaccines and immunotherapies. Different VIAs that differ in length of CD8:CD4 T cell culture periods (6–13 days), purity of CD4 cultures

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    A Combinatorial in-silico, in-vitro and in-vivo Approach to Quantitatively Study Peptide Induced MHC Stability
    结合硅、体外和体内方法定量研究多肽诱导的MHC稳定性
    作者:Roman Sarkar, Yashu Sharma, Ayush Jain, Azeez Tehseen, Sudhakar Singh and Sharvan Sehrawat日期:12/20/2021,浏览量:1140,Q&A: 0
    [Abstract]

    Here, we describe a combinatorial approach in reverse vaccinology to identify immunogenic class I major histocompatibility complex (MHC) displayed epitopes derived from a morbillivirus named pestes des petits ruminants (PPRV). The protocol describes an in silico prediction of immunogenic epitopes using an IEDB tool. The predicted peptides were

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    Fixation and Immunostaining of Endogenous Proteins or Post-translational Modifications in Caenorhabditis elegans
    秀丽隐杆线虫内源性蛋白质的固定和免疫染色或翻译后修饰
    作者:Robert O'Hagan and Irini Topalidou日期:10/05/2021,浏览量:1744,Q&A: 0
    [Abstract]

    Although the advent of genetically-encoded fluorescent markers, such as the green fluorescent protein (GFP; Chalfie et al., 1994), has enabled convenient visualization of gene expression in vivo, this method is generally not effective for detecting post-translational modifications because they are not translated from DNA sequences.

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    Identification and Quantitation of Neutrophil Extracellular Traps in Human Tissue Sections
    人体组织切片中中性粒细胞胞外陷阱的鉴定与定量
    作者:Coraline Radermecker, Alexandre Hego, Philippe Delvenne and Thomas Marichal日期:09/20/2021,浏览量:2125,Q&A: 0
    [Abstract]

    Neutrophils are one of the first innate immune cells recruited to tissues during inflammation. An important function of neutrophils relies on their ability to release extracellular structures, known as Neutrophil Extracellular Traps or NETs, into their environment. Detecting such NETs in humans has often proven challenging for both biological

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    Isolation and Quantification of Mouse γδT-cells in vitro and in vivo
    体外和体内小鼠γδT细胞的分离和定量
    作者:Isha Rana, Krithika Badarinath, Ravindra K. Zirmire and Colin Jamora日期:09/05/2021,浏览量:1872,Q&A: 0
    [Abstract]

    The skin plays an important role in protecting the body from pathogens and chemicals in the external environment. Upon injury, a healing program is rapidly initiated and involves extensive intercellular communication to restore tissue homeostasis. The deregulation of this crosstalk can lead to abnormal healing processes and is the foundation of

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    One-step White Blood Cell Extracellular Staining Method for Flow Cytometry
    流式细胞术白细胞细胞外一步染色法
    作者:Ines Ait Belkacem, Pénélope Bourgoin, Jean Marc Busnel, Franck Galland and Fabrice Malergue日期:08/20/2021,浏览量:1646,Q&A: 0
    [Abstract]

    Flow cytometry is a powerful analytical technique that is increasingly used in scientific investigations and healthcare; however, it requires time-consuming, multi-step sample procedures, which limits its use to specialized laboratories. In this study, we propose a new universal one-step method in which white blood cell staining and red blood cell

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    Multi-color Flow Cytometry for Comprehensive Analysis of the Tumor Immune Infiltrate in a Murine Model of Breast Cancer
    多色流式细胞术综合分析乳腺癌小鼠模型中肿瘤免疫渗透
    作者:Ana S. Almeida, Miriam R. Fein and Mikala Egeblad日期:06/05/2021,浏览量:3139,Q&A: 0
    [Abstract]

    Flow cytometry is a popular laser-based technology that allows the phenotypic and functional characterization of individual cells in a high-throughput manner. Here, we describe a detailed procedure for preparing a single-cell suspension from mammary tumors of the mouse mammary tumor virus-polyoma middle T (MMTV-PyMT) and analyzing these cells by

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