植物科学

分类

    现刊
    Colocalization Assay with Fluorescent-tagged ATG8 Using a Nicotiana benthamiana-based Transient System
    本氏烟草的瞬态系统进行荧光标记 ATG8 的共定位分析
    作者:Jinyan Mai, Dandan Shang, Faqiang Li and Na Luo日期:08/20/2022,浏览量:1052,Q&A: 0
    [Abstract]

    Autophagy is an evolutionarily conserved intracellular degradation process. During autophagy, a set of autophagy-related (ATG) proteins orchestrate the formation of double-bound membrane vesicles called autophagosomes to engulf cytoplasmic material and deliver it to the vacuole for breakdown. Among ATG proteins, the ATG8 is the only one decorating

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    Fractionation and Extraction of Crude Nuclear Proteins From Arabidopsis Seedlings
    拟南芥幼苗核蛋白的分离与提取
    作者:Jiajia Zhao, Jinsong Bao and Feifei Xu日期:01/20/2022,浏览量:2228,Q&A: 0
    [Abstract]

    The plant nucleus is an important subcellular organelle that contains the genome, ribosomal RNA, and regulatory proteins, and performs a central role in the functioning and metabolism of the cell. Fractionation of intact nuclei is a crucial process to elucidate the function of nuclear proteins. Here, we present a simple method for the

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    Imaging of Lipid Uptake in Arabidopsis Seedlings Utilizing Fluorescent Lipids and Confocal Microscopy
    利用荧光脂质和共聚焦显微镜对拟南芥幼苗的脂质摄取进行成像
    作者:Rosa L. López-Marqués and Thomas G. Pomorski日期:11/20/2021,浏览量:2268,Q&A: 0
    [Abstract]

    Eukaryotic cells use a diverse set of transporters to control the movement of lipids across their plasma membrane, which drastically affects membrane properties. Various tools and techniques to analyze the activity of these transporters have been developed. Among them, assays based on fluorescent phospholipid probes are particularly suitable,

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    Preparation and Transfection of Populus tomentosa Mesophyll Protoplasts
    杨树中间叶原生质体的制备和转染
    作者:Hou-Ling Wang, Ting Wang, Qi Yang, Weilun Yin, Xinli Xia, Hongwei Guo and Zhonghai Li日期:11/20/2021,浏览量:2351,Q&A: 0
    [Abstract]

    Mesophyll protoplasts freshly isolated from leaves are a useful research system in plants. However, cell walls in woody plants contain more pectin, making mesophyll protoplasts isolation difficult in Populus. This has limited their application in biochemical, molecular, cellular, genetic, genomic, transcriptomic, and proteomic assays. In this

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    Enrichment of Cytoplasmic RNA Granules from Arabidopsis Seedlings
    从拟南芥幼苗中富集细胞质 RNA 颗粒
    作者:Zhen Lei, Eun Yu Kim and Jungnam Cho日期:11/05/2021,浏览量:1879,Q&A: 0
    [Abstract]

    RNA granules (RGs) are membraneless intracellular compartments that play important roles in the post-transcriptional control of gene expression. Stress granules (SGs) are a type of RGs that form under environmental challenges and/or internal cellular stresses. Stress treatments lead to strong mRNAs translational inhibition and storage in SGs until

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    High-throughput Screening for Defense Priming-inducing Compounds in Parsley Cell Cultures
    欧芹细胞防御引物诱导化合物的高通量筛选
    作者:Kathrin Schmitz, Linda Werner and Uwe Conrath日期:10/20/2021,浏览量:1570,Q&A: 0
    [Abstract]

    Defense priming describes the enhanced potency of cells to activate defense responses. Priming accompanies local and systemic immune responses and can be triggered by microbial infection or upon treatment with certain chemicals. Thus, chemically activating defense priming is promising for biomedicine and agriculture. However, test systems for

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    A Novel Method to Map Small RNAs with High Resolution
    一种新的高分辨率小RNA图谱绘制方法
    作者:Kun Huang, Feray Demirci, Blake C. Meyers and Jeffrey L. Caplan日期:08/20/2021,浏览量:2445,Q&A: 0
    [Abstract]

    Analyzing cellular structures and the relative location of molecules is essential for addressing biological questions. Super-resolution microscopy techniques that bypass the light diffraction limit have become increasingly popular to study cellular molecule dynamics in situ. However, the application of super-resolution imaging techniques to detect

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    Peptide-mediated Targeting of Nanoparticles with Chemical Cargoes to Chloroplasts in Arabidopsis Plants
    多肽介导的化学物质纳米粒对拟南芥叶绿体的靶向作用
    作者:Israel Santana, Peiguang Hu, Su-Ji Jeon, Chris Castillo, Hann Tu and Juan Pablo Giraldo日期:06/20/2021,浏览量:2618,Q&A: 0
    [Abstract]

    Plant nanobiotechnology is a flourishing field that uses nanomaterials to study and engineer plant function. Applications of nanotechnology in plants have great potential as tools for improving crop yield, tolerance to disease and environmental stress, agrochemical delivery of pesticides and fertilizers, and genetic modification and transformation

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    A Pulse–chase EdU Method for Detection of Cell Division Orientation in Arabidopsis and Juncus prismatocarpus Leaf Primordia
    脉冲追踪EdU法检测拟南芥和笄石菖叶原基细胞分裂方向
    作者:Xiaofeng Yin and Hirokazu Tsukaya日期:01/05/2021,浏览量:2578,Q&A: 0
    [Abstract] In plants, the morphological diversity of leaves is largely determined by cell division, especially cell division orientation. Whereas cell division itself is easily monitored, the detection and quantification of cell division orientation are difficult. The few existing methods for detection and quantification of cell division orientation are ...
    Lipid Droplet Isolation from Arabidopsis thaliana Leaves
    拟南芥叶脂滴的分离
    作者:Yovanny Izquierdo, Rubén Fernández-Santos, Tomás Cascón and Carmen Castresana日期:12/20/2020,浏览量:2643,Q&A: 0
    [Abstract]

    Lipid droplets (LDs) are neutral lipid aggregates surrounded by a phospholipid monolayer and specific proteins. In plants, they play a key role as energy source after seed germination, but are also formed in vegetative tissues in response to developmental or environmental conditions, where their functions are poorly understood. To elucidate these,

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