联合作者(
Atin Sharma The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University
2 protocols

Philippe J. Sansonetti Institut Pasteur, Unité de Pathogénie Microbienne Moléculaire, France
4 protocols
联合评审(
Aksiniya Asenova University of California, Davis
12 protocols

Alexander B Westbye NIOZ Royal Netherlands Institute for Sea Research
6 protocols

Alexandros C Kokotos Weill Cornell Medicine
7 protocols

Ana Santos Almeida University College Cork
4 protocols

编委
Andrea Puhar
  • Faculty, The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University, Sweden
研究方向
  • 免疫学
  • Infection; Inflammation; Endogenous danger signals; Extracellular ATP; Intestinal bacteria; Pathogens; Microbiota; Gut
  • 已发表 protocol 4
课题组

教育背景

Ph.D., University of Padova, Italy, 2007

实验室信息

Andrea Puhar Lab
http://www.mims.umu.se/groups/andrea-puhar.html

发表论文

已发表 protocol 4篇
Plaque Assay to Determine Invasion and Intercellular Dissemination of Shigella flexneri in TC7 Human Intestinal Epithelial Cells
蚀斑测定弗氏志贺在TC7人肠上皮细胞中的侵袭和细胞间传播

作者:Atin Sharma and Andrea Puhar日期:07/05/2019,浏览量:233,Q&A: 0
Shigella flexneri invades the epithelial cells lining the gut lumen and replicates intracellularly. The specialized Type III Secretion System (T3SS) and its effector proteins, encoded on a large virulence plasmid, assist the bacterium to ...
Gentamicin Protection Assay to Determine the Number of Intracellular Bacteria during Infection of Human TC7 Intestinal Epithelial Cells by Shigella flexneri
庆大霉素保护试验确定福氏志贺菌侵染人TC7肠上皮细胞的胞内寄生数目

作者:Atin Sharma and Andrea Puhar日期:07/05/2019,浏览量:275,Q&A: 0
Shigella flexneri is an intracellular bacterial pathogen that gains access to the gut epithelium using a specialized Type III Secretion System (T3SS). Various determinants mediating this invasive infection have been experimentally verified ...
参审 protocol 6篇
Quantification of Neisseria meningitidis Adherence to Human Epithelial Cells by Colony Counting
通过菌落计数定量脑膜炎奈瑟菌对人上皮细胞的粘附能力
作者:Sara Sigurlásdóttir, Sunil D Saroj, Olaspers Sara Eriksson, Jens Eriksson and Ann-Beth Jonsson日期:02/05/2018,浏览量:2560,Q&A: 0
To cause an infection, the human specific pathogen Neisseria meningitides must first colonize the nasopharynx. Upon tight interaction with the mucosal epithelium, N. meningitidis may cross the epithelial cellular barrier, reach the ...
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Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles
使用精氨酸功能化金纳米粒子进行CRISPR/Cas9-核糖核蛋白的细胞溶质和细胞核递送以用于基因编辑
作者:Rubul Mout and Vincent M. Rotello日期:10/20/2017,浏览量:5804,Q&A: 1
In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is ...
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主审 protocol 37篇
Adhesive and Cohesive Peel Force Measurement of Human Airway Mucus
人气道粘液的黏附力和粘结剥离力测定
In health, the high-speed airflow associated with cough represents a vital backup mechanism for clearing accumulated mucus from our airways. However, alterations in the mucus layer in cystic fibrosis (CF) and chronic obstructive pulmonary disease ...
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Tryptophan Fluorescence Quenching Assays for Measuring Protein-ligand Binding Affinities: Principles and a Practical Guide
用于检测蛋白配体结合亲和力的色氨酸荧光猝灭试验:原理和操作指南
作者:Anthony Yammine, Jinlong Gao and Ann H. Kwan日期:06/05/2019,浏览量:756,Q&A: 0
Tryptophan fluorescence quenching is a type of fluorescence spectroscopy used for binding assays. The assay relies on the ability to quench the intrinsic fluorescence of tryptophan residues within a protein that results from changes in the local ...
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