联合作者(
Ramón Cervantes-Rivera The Laboratory For Molecular Infection Medicine Sweden (MIMS) and Department of Molecular Biology, Umeå University, Umeå
1 protocol

Atin Sharma The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University
2 protocols

Philippe J. Sansonetti Institut Pasteur, Unité de Pathogénie Microbienne Moléculaire, France
4 protocols
联合评审(
Aksiniya Asenova University of California, Davis
15 protocols

Alexander B Westbye NIOZ Royal Netherlands Institute for Sea Research
6 protocols

Alexandros C Kokotos Weill Cornell Medicine
18 protocols

Ana S. Almeida University College Cork
8 protocols

编委
Andrea Puhar
  • Faculty, MIMS and Department of Molecular Biology, Umeå University
研究方向
  • 微生物学
  • Infection; Inflammation; Endogenous danger signals; Extracellular ATP; Intestinal bacteria; Pathogens; Microbiota; Gut
  • 已发表 protocol 5
课题组

教育背景

Ph.D., University of Padova, Italy, 2007

实验室信息

Andrea Puhar Lab
http://www.mims.umu.se/groups/andrea-puhar.html

发表论文

已发表 protocol 5篇
Whole-genome Identification of Transcriptional Start Sites by Differential RNA-seq in Bacteria
细菌转录起始位点的差异RNA序列全基因组鉴定

作者:Ramón Cervantes-Rivera and Andrea Puhar日期:09/20/2020,浏览量:2986,Q&A: 0
Gene transcription in bacteria often starts some nucleotides upstream of the start codon. Identifying the specific Transcriptional Start Site (TSS) is essential for genetic manipulation, as in many cases upstream of the start codon there are ...
Plaque Assay to Determine Invasion and Intercellular Dissemination of Shigella flexneri in TC7 Human Intestinal Epithelial Cells
蚀斑测定弗氏志贺在TC7人肠上皮细胞中的侵袭和细胞间传播

作者:Atin Sharma and Andrea Puhar日期:07/05/2019,浏览量:3161,Q&A: 0
Shigella flexneri invades the epithelial cells lining the gut lumen and replicates intracellularly. The specialized Type III Secretion System (T3SS) and its effector proteins, encoded on a large virulence plasmid, assist the bacterium to ...
参审 protocol 6篇
Quantification of Neisseria meningitidis Adherence to Human Epithelial Cells by Colony Counting
通过菌落计数定量脑膜炎奈瑟菌对人上皮细胞的粘附能力
作者:Sara Sigurlásdóttir, Sunil D Saroj, Olaspers Sara Eriksson, Jens Eriksson and Ann-Beth Jonsson日期:02/05/2018,浏览量:6028,Q&A: 0
To cause an infection, the human specific pathogen Neisseria meningitides must first colonize the nasopharynx. Upon tight interaction with the mucosal epithelium, N. meningitidis may cross the epithelial cellular barrier, reach the ...
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Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles
使用精氨酸功能化金纳米粒子进行CRISPR/Cas9-核糖核蛋白的细胞溶质和细胞核递送以用于基因编辑
作者:Rubul Mout and Vincent M. Rotello日期:10/20/2017,浏览量:9779,Q&A: 1
In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is ...
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主审 protocol 47篇
Microscopic Detection of ASC Inflammasomes in Bone Marrow Derived Macrophages Post Stimulation
刺激后骨髓源性巨噬细胞中ASC炎症小体的显微检测
作者:Longjun Li, Rudi Mao and Xiaopeng Qi日期:09/05/2021,浏览量:671,Q&A: 0

An inflammasome is an intracellular multiprotein complex that plays important roles in host defense and inflammatory responses. Inflammasomes are typically composed of the adaptor protein apoptosis-associated speck-like protein containing a CARD

...
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Chromatographic Assays for the Enzymatic Degradation of Chitin
几丁质酶解的色谱分析
作者:Sophanit Mekasha, Tina R. Tuveng, Gustav Vaaje-Kolstad and Vincent G. H. Eijsink日期:05/05/2021,浏览量:1957,Q&A: 0

Chitin is an insoluble linear polymer of β(1→4)-linked N-acetylglucosamine. Enzymatic cleavage of chitin chains can be achieved using hydrolytic enzymes, called chitinases, and/or oxidative enzymes, called lytic polysaccharide

...
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