联合作者(
Atin Sharma The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University
2 protocols

Philippe J. Sansonetti Institut Pasteur, Unité de Pathogénie Microbienne Moléculaire, France
4 protocols
联合评审(
Aksiniya Asenova University of California, Davis
14 protocols

Alexander B Westbye NIOZ Royal Netherlands Institute for Sea Research
6 protocols

Alexandros C Kokotos Weill Cornell Medicine
13 protocols

Ana Santos Almeida University College Cork
6 protocols

编委
Andrea Puhar
  • Faculty, The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University, Sweden
研究方向
  • 免疫学
  • Infection; Inflammation; Endogenous danger signals; Extracellular ATP; Intestinal bacteria; Pathogens; Microbiota; Gut
  • 已发表 protocol 4
课题组

教育背景

Ph.D., University of Padova, Italy, 2007

实验室信息

Andrea Puhar Lab
http://www.mims.umu.se/groups/andrea-puhar.html

发表论文

已发表 protocol 4篇
Plaque Assay to Determine Invasion and Intercellular Dissemination of Shigella flexneri in TC7 Human Intestinal Epithelial Cells
蚀斑测定弗氏志贺在TC7人肠上皮细胞中的侵袭和细胞间传播

作者:Atin Sharma and Andrea Puhar日期:07/05/2019,浏览量:1415,Q&A: 0
Shigella flexneri invades the epithelial cells lining the gut lumen and replicates intracellularly. The specialized Type III Secretion System (T3SS) and its effector proteins, encoded on a large virulence plasmid, assist the bacterium to ...
Gentamicin Protection Assay to Determine the Number of Intracellular Bacteria during Infection of Human TC7 Intestinal Epithelial Cells by Shigella flexneri
庆大霉素保护试验确定福氏志贺菌侵染人TC7肠上皮细胞的胞内寄生数目

作者:Atin Sharma and Andrea Puhar日期:07/05/2019,浏览量:2264,Q&A: 0
Shigella flexneri is an intracellular bacterial pathogen that gains access to the gut epithelium using a specialized Type III Secretion System (T3SS). Various determinants mediating this invasive infection have been experimentally verified ...
参审 protocol 6篇
Quantification of Neisseria meningitidis Adherence to Human Epithelial Cells by Colony Counting
通过菌落计数定量脑膜炎奈瑟菌对人上皮细胞的粘附能力
作者:Sara Sigurlásdóttir, Sunil D Saroj, Olaspers Sara Eriksson, Jens Eriksson and Ann-Beth Jonsson日期:02/05/2018,浏览量:4245,Q&A: 0
To cause an infection, the human specific pathogen Neisseria meningitides must first colonize the nasopharynx. Upon tight interaction with the mucosal epithelium, N. meningitidis may cross the epithelial cellular barrier, reach the ...
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Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles
使用精氨酸功能化金纳米粒子进行CRISPR/Cas9-核糖核蛋白的细胞溶质和细胞核递送以用于基因编辑
作者:Rubul Mout and Vincent M. Rotello日期:10/20/2017,浏览量:7502,Q&A: 1
In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is ...
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主审 protocol 41篇
Assessing in vitro and in vivo Trogocytosis By Murine CD4+ T cells
用小鼠CD4+T细胞评价体内外胞啃作用
作者:Jim Reed and Scott A. Wetzel日期:05/05/2020,浏览量:553,Q&A: 0
Recognition of antigens by lymphocytes (B, T, and NK) on the surface of an antigen-presenting cell (APC) leads to lymphocyte activation and the formation of an immunological synapse between the lymphocyte and the APC. At the immunological synapse ...
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Method for Measuring Mucociliary Clearance and Cilia-generated Flow in Mice by ex vivo Imaging
小鼠中黏膜纤毛清除和纤毛产生流动的离体成像检测方法
作者:Eric Song and Akiko Iwasaki日期:03/20/2020,浏览量:405,Q&A: 0
Ex vivo biophysical measurements provide valuable insights into understanding both physiological and pathogenic processes. One critical physiological mechanism that is regulated by these biophysical properties is cilia-generated flow that ...
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