联合评审(
Antoine de Morree Stanford university
36 protocols

Fanglian He Bio-protocol
205 protocols

Ignacio Lescano Centro de Investigaciones en Química Biológica de Córdoba
4 protocols

Lin Fang Stanford University
95 protocols

编委
Hui Zhu
  • Stanford University
研究方向
  • 细胞生物学
  • 已发表 protocol 9
课题组
已发表 protocol 9篇
Cell Cycle Analysis Using Propidium Iodide Staining with GFP Detection
用GFP检测细胞周期分析(PI)

作者:Hui Zhu日期:04/05/2012,浏览量:22959,Q&A: 3
Infecting mammalian cells with a GFP construct to overexpress or knockdown target genes is one of the most commonly used methods to study and manipulate gene expression. To determine the target gene function on the cell cycle, analyzing the cell ...
Cell Proliferation Assay by Flow Cytometry (BrdU and PI Staining)
流式细胞术检测细胞增殖(BrdU和 PI 染色)

作者:Hui Zhu日期:04/05/2012,浏览量:65189,Q&A: 2
Cell Proliferation assays include an important set of fluorescence-based tests that can monitor cell health and cell division by evaluating DNA synthesis through thymidine incorporation. Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a ...
参审 protocol 17篇
Isolation of Primary Human Skeletal Muscle Cells
原代人骨骼肌细胞的分离
作者:Janelle M. Spinazzola and Emanuela Gussoni日期:11/05/2017,浏览量:6877,Q&A: 0
Primary myoblast culture is a valuable tool in research of muscle disease, pathophysiology, and pharmacology. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic ...
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Isolation of Keratan Sulfate Disaccharide-branched Chondroitin Sulfate E from Mactra chinensis
中国蛤蜊中硫酸角质素二糖-支链硫酸软骨素E的分离
作者:Kyohei Higashi and Toshihiko Toida日期:08/05/2017,浏览量:5499,Q&A: 0
Glycosaminoglycans (GAGs) including chondroitin sulfate (CS), dermatan sulfate (DS), heparin (HP), heparan sulfate (HS) and keratan sulfate (KS) are linear, sulfated repeating disaccharide sequences containing hexosamine and uronic acid (or ...
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主审 protocol 1篇
Advances in Proximity Ligation in situ Hybridization (PLISH)
PLISH技术的研究进展
作者:Monica Nagendran, Adam M Andruska, Pehr B Harbury and Tushar J Desai日期:11/05/2020,浏览量:2494,Q&A: 0
Understanding tissues in the context of development, maintenance and disease requires determining the molecular profiles of individual cells within their native in vivo spatial context. We developed a Proximity Ligation in situ ...
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