联合作者(
Rachael Marie Giersch Biochemistry and Molecular Biophysics, Kansas State University, USA, USA,
1 protocol

Jeremy Thorner Division of Biochemistry, Biophysics and Structural Biology, Department of Molecular and Cell Biology, University of California, USA
1 protocol
联合评审(
Joëlle Schlapfer Lawrence Berkeley National Laboratory
8 protocols

Melike Çağlayan University of Florida
3 protocols

Ralph Thomas Boettcher Max Planck Institute for Biochemistry
35 protocols

评审
Gregory C. Finnigan
  • Faculty, Kansas State University
研究方向
  • 分子生物学
  • 已发表 protocol 2
已发表 protocol 2篇
Method for Multiplexing CRISPR/Cas9 in Saccharomyces cerevisiae Using Artificial Target DNA Sequences
酿酒酵母中使用人工靶DNA序列进行多重CRISPR/ Cas9的方法

作者:Rachael M. Giersch and Gregory C. Finnigan日期:09/20/2017,浏览量:4127,Q&A: 0
Genome manipulation has become more accessible given the advent of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) editing technology. The Cas9 endonuclease binds a single stranded (single guide) RNA (sgRNA) fragment that ...
Complex in vivo Ligation Using Homologous Recombination and High-efficiency Plasmid Rescue from Saccharomyces cerevisiae
通过同源重组进行复合体的体内连接及在酿酒酵母中进行高效质粒拯救

作者:Gregory C. Finnigan and Jeremy Thorner日期:07/05/2015,浏览量:5343,Q&A: 0
The protocols presented here allow for the facile generation of a wide variety of complex multipart DNA constructs (tagged gene products, gene fusions, chimeric proteins, and other variants) using homologous recombination and in vivo ...
参审 protocol 2篇
Trypsin Sensitivity Assay to Study the Folding Status of Proteins
胰蛋白酶敏感性试验研究蛋白质的折叠状态
作者:Satoshi Ninagawa and Kazutoshi Mori日期:10/05/2016,浏览量:3846,Q&A: 0
This protocol aims to evaluate folding status of proteins, utilizing trypsin sensitivity. Unfolded/misfolded proteins are more susceptible to trypsin than folded proteins, because trypsin easily accesses and cleaves loosely folded parts of proteins. ...
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PNGase Sensitivity Assay to Study the Folding Status of Proteins
聚糖酶敏感性试验研究蛋白质的折叠状态
作者:Satoshi Ninagawa and Kazutoshi Mori日期:10/05/2016,浏览量:3146,Q&A: 0
This protocol aims to evaluate folding status of proteins, utilizing peptide:N-glycanase (PNGase) sensitivity. In the cytosol, PNGase works as a deglycosylation-enzyme. N-glycans on unfolded/misfolded proteins are more susceptible to PNGase than ...
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