联合作者(
Kedar Narayan Center for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, NIH Frederick
1 protocol

Irene Y. Chang Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD
1 protocol

Mohammad Rahman NIH Bethesda
2 protocols

Orna Cohen-Fix
  • National Institute for Diabetes and Digestive and Kidney Diseases, NIH Bethesda
  • 已发表 protocol 1
已发表 protocol 1篇
A Workflow for High-pressure Freezing and Freeze Substitution of the Caenorhabditis elegans Embryo for Ultrastructural Analysis by Conventional and Volume Electron Microscopy
用常规和体积电子显微镜对秀丽隐杆线虫胚胎进行超微结构分析的高压冷冻和冷冻替代的工作流程

作者:Mohammad M. Rahman, Irene Y. Chang, Orna Cohen-Fix and Kedar Narayan日期:04/05/2021,浏览量:145,Q&A: 0

The free-living nematode Caenorhabditis elegans is a popular model system for studying developmental biology. Here we describe a detailed protocol to high-pressure freeze the C. elegans embryo (either ex vivo after dissection, or within the intact

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