联合作者(
Khaled A. Selim Tübingen University Tübingen
1 protocol

Karl Forchhammer
2 protocols

Tim Orthwein
1 protocol

Luciano F. Huergo
  • Faculty,
  • 已发表 protocol 1
已发表 protocol 1篇
Kinetic Analysis of a Protein-protein Complex to Determine its Dissociation Constant (KD) and the Effective Concentration (EC50) of an Interplaying Effector Molecule Using Bio-layer Interferometry
蛋白质-蛋白质复合物的动力学分析,以确定其电离常数 (KD) 和使用生物层干涉法相互作用的效应分子的有效浓度 (EC50)

作者:Tim Orthwein, Luciano F. Huergo, Karl Forchhammer and Khaled A. Selim日期:09/05/2021,浏览量:1460,Q&A: 0

Biolayer interferometry (BLI) is an emerging analytical tool that allows the study of protein complexes in real time to determine protein complex kinetic parameters. This article describes a protocol to determine the KD of a protein complex using a

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