联合作者(
Yuhui Wang Hainan University Haikou
1 protocol

Kang Li Hainan University Haikou
1 protocol

Chuanying Fang
  • Research scientist, Hainan University Haikou
  • 已发表 protocol 1
已发表 protocol 1篇
A Novel Method to Construct Binary CRISPR Vectors for Plant Transformation by Single Round of PCR Amplification
一种通过单轮PCR扩增构建用于植物转化的二元CRISPR载体的新方法

作者:Kang Li, Yuhui Wang and Chuanying Fang日期:04/05/2021,浏览量:379,Q&A: 0

CRISPR/Cas9 is an established and flexible tool for genome editing. However, most methods used to generate expression clones for the CRISPR/Cas9 are time-consuming. Hence, we have developed a one-step protocol to introduce sgRNA expression

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