联合作者(
Holden T. Maecker Immunity, Transplantation & Infection, School of Medicine, Stanford University, Stanford
9 protocols

Sangeeta Kowli
  • 已发表 protocol 1
已发表 protocol 1篇
Immunophenotyping and Intracellular Staining of Fixed Whole Blood for Mass Cytometry (CyTOF)
用于细胞计数法(CyTOF)的固定全血的免疫分型和细胞内染色

作者:Sangeeta Kowli and Holden Maecker日期:09/05/2020,浏览量:3055,Q&A: 0
In this report, we present the implementation of mass cytometry for intracellular staining using fixed whole blood. In our assay described here, 250 µl of whole blood, is stimulated in vitro with PMA/ionomycin (or left unstimulated), in the ...