联合作者(
Dario Krapf Laboratory of Cell Signal Transduction Networks, Instituto de Biologia Molecular y Celular de Rosario, Rosario
1 protocol

Pablo E. Visconti Department of Veterinary and Animal Sciences, Umass, Amherst
1 protocol

Carolina Baro Graf Laboratoty of Cell Signal Transduction Network, Institute of Molecular and Cellular Biology of Rosario (IBR), 2016-
1 protocol

Cintia Stival
  • Research Associate, Cell Signal Transduction Networks, Institute of Molecular and Cellular Biology of Rosario
研究方向
  • -
  • 已发表 protocol 1

教育背景

PhD in Biological Sciences, Universidad Nacional de Rosario

发表论文

• Baro Graf, C., Ritagliati, C., Torres-Monserrat, V., Stival, C., Carizza, C., Buffone, M. G. and Krapf, D. (2019). Membrane Potential Assessment by Fluorimetry as a Predictor Tool of Human Sperm Fertilizing Capacity. Front Cell Dev Biol 7: 383.
• Baro Graf, C., Ritagliati, C., Stival, C., Balestrini, P. A., Buffone, M. G. and Krapf, D. (2019). Determination of a Robust Assay for Human Sperm Membrane Potential Analysis. Front Cell Dev Biol 7: 101.
• Ritagliati, C., Luque, G. M., Stival, C., Baro Graf, C., Buffone, M. G. and Krapf, D. (2018). Lysine acetylation modulates mouse sperm capacitation. Sci Rep 8(1): 13334.
• Stival, C., Ritagliati, C., Xu, X., Gervasi, M. G., Luque, G. M., Baro Graf, C., De la Vega-Beltran, J. L., Torres, N., Darszon, A., Krapf, D., Buffone, M. G., Visconti, P. E. and Krapf, D. (2018). Disruption of protein kinase A localization induces acrosomal exocytosis in capacitated mouse sperm. J Biol Chem 293(24): 9435-9447.
• Ritagliati, C., Baro Graf, C., Stival, C. and Krapf, D. (2018). Regulation mechanisms and implications of sperm membrane hyperpolarization. Mech Dev 154: 33-43.
• González, L. J., Stival, C., Puzzolo, J. L., Moreno, D. M. and Vila, A. J. (2018). Shaping Substrate Selectivity in a Broad-Spectrum Metallo-beta-Lactamase. Antimicrob Agents Chemother 62(4).
• Stival, C., Puga Molina Ldel, C., Paudel, B., Buffone, M. G., Visconti, P. E. and Krapf, D. (2016). Sperm Capacitation and Acrosome Reaction in Mammalian Sperm. Adv Anat Embryol Cell Biol 220: 93-106.
• La Spina, F. A., Stival, C., Krapf, D. and Buffone, M. G. (2017). Molecular and Cellular Aspects of Mammalian Sperm Acrosomal Exocytosis. In H. Schatten & GM. Constantinescu (Eds.), Animal Models and Human Reproduction (pp. 409–426). Hoboken, New Jersey: John Wiley & Sons Inc.
• Stival, C., La Spina, F. A., Baro Graf, C., Arcelay, E., Arranz, S. E., Ferreira, J. J., Le Grand, S., Dzikunu, V. A., Santi, C. M., Visconti, P. E., Buffone, M. G. and Krapf, D. (2015). Src Kinase Is the Connecting Player between Protein Kinase A (PKA) Activation and Hyperpolarization through SLO3 Potassium Channel Regulation in Mouse Sperm. J Biol Chem 290(30): 18855-18864.
已发表 protocol 1篇
Quantification of Protein Kinase A (PKA) Activity by An in vitro Radioactive Assay Using the Mouse Sperm Derived Enzyme
用小鼠精子衍生酶体外放射性测定蛋白激酶A(PKA)活性

作者:Cintia Stival, Carolina Baro Graf, Pablo E. Visconti and Dario Krapf日期:06/20/2020,浏览量:2424,Q&A: 0
In order to acquire fertilizing potential, mammalian sperm must undergo a process known as capacitation, which relies on the early activation of Protein Kinase A (PKA). Frequently, PKA activity is assessed in whole-cell ...