联合作者(
Enako Hosoda Science and Education Center Ochanomizu University
1 protocol

Kazuyoshi Chiba
  • Professor, Biological Sciences Ochanomizu University
研究方向
  • 发育生物学
  • Oocyte maturation, Fertilization, Maternal mRNA, Apoptosis
  • 已发表 protocol 1

教育背景

DSc, DSc, 1990 1990

实验室信息

Chiba lab.

发表论文

1. Chiba, K. Oocyte Maturation in Starfish. Cells 2020, 9 (2), 476. https://doi.org/10.3390/cells9020476.
2. Hosoda, E.; Hiraoka, D.; Hirohashi, N.; Omi, S.; Kishimoto, T.; Chiba, K. SGK Regulates pH Increase and Cyclin B–Cdk1 Activation to Resume Meiosis in Starfish Ovarian Oocytes. J. Cell Biol. 2019, 218 (11), 3612–3629. https://doi.org/10.1083/jcb.201812133.
3. Hiraoka, D.; Hosoda, E.; Chiba, K.; Kishimoto, T. SGK Phosphorylates Cdc25 and Myt1 to Trigger Cyclin B-Cdk1 Activation at the Meiotic G2/M Transition. J. Cell Biol. 2019, 218 (11), 3597–3611. https://doi.org/10.1083/jcb.201812122.
4. Tamura, R.; Takada, M.; Sakaue, M.; Yoshida, A.; Ohi, S.; Hirano, K.; Hayakawa, T.; Hirohashi, N.; Yura, K.; Chiba, K. Starfish Apaf-1 Activates Effector Caspase-3/9 upon Apoptosis of Aged Eggs. Sci. Rep. 2018, 8 (1), 1611. https://doi.org/10.1038/s41598-018-19845-6.
5. Ochi, H.; Chiba, K. Hormonal Stimulation of Starfish Oocytes Induces Partial Degradation of the 3’ Termini of Cyclin B MRNAs with Oligo(U) Tails, Followed by Poly(A) Elongation. RNA N. Y. N 2016, 22 (6), 822–829. https://doi.org/10.1261/rna.054882.115.
6. Ochi, H.; Aoto, S.; Tachibana, K.; Hara, M.; Chiba, K. Block of CDK1-Dependent Polyadenosine Elongation of Cyclin B MRNA in Metaphase-i-Arrested Starfish Oocytes Is Released by Intracellular pH Elevation upon Spawning. Mol. Reprod. Dev. 2016, 83 (1), 79–87. https://doi.org/10.1002/mrd.22599.
7. Moriwaki, K.; Nakagawa, T.; Nakaya, F.; Hirohashi, N.; Chiba, K. Arrest at Metaphase of Meiosis I in Starfish Oocytes in the Ovary Is Maintained by High CO2 and Low O2 Concentrations in Extracellular Fluid. Zoolog. Sci. 2013, 30 (11), 975–984. https://doi.org/10.2108/zsj.30.975.
8. Hirohashi, N.; Alvarez, L.; Shiba, K.; Fujiwara, E.; Iwata, Y.; Mohri, T.; Inaba, K.; Chiba, K.; Ochi, H.; Supuran, C. T.; Kotzur, N.; Kakiuchi, Y.; Kaupp, U. B.; Baba, S. A. Sperm from Sneaker Male Squids Exhibit Chemotactic Swarming to CO₂. Curr. Biol. CB 2013, 23 (9), 775–781. https://doi.org/10.1016/j.cub.2013.03.040.
9. Otsuki, J.; Nagai, Y.; Lopata, A.; Chiba, K.; Yasmin, L.; Sankai, T. Symmetrical Division of Mouse Oocytes during Meiotic Maturation Can Lead to the Development of Twin Embryos That Amalgamate to Form a Chimeric Hermaphrodite. Hum. Reprod. Oxf. Engl. 2012, 27 (2), 380–387. https://doi.org/10.1093/humrep/der408.
10. Jin, M.; Fujiwara, E.; Kakiuchi, Y.; Okabe, M.; Satouh, Y.; Baba, S. A.; Chiba, K.; Hirohashi, N. Most Fertilizing Mouse Spermatozoa Begin Their Acrosome Reaction before Contact with the Zona Pellucida during in Vitro Fertilization. Proc. Natl. Acad. Sci. U. S. A. 2011, 108 (12), 4892–4896. https://doi.org/10.1073/pnas.1018202108.
11. Chiba, K. Evolution of the Acquisition of Fertilization Competence and Polyspermy Blocks during Meiotic Maturation. Mol. Reprod. Dev. 2011, 78 (10–11), 808–813. https://doi.org/10.1002/mrd.21378.
12. Harada, K.; Fukuda, E.; Hirohashi, N.; Chiba, K. Regulation of Intracellular pH by P90Rsk-Dependent Activation of an Na+/H+ Exchanger in Starfish Oocytes. J. Biol. Chem. 2010, 285 (31), 24044–24054. https://doi.org/10.1074/jbc.M109.072553.
13. Otsuki, J.; Nagai, Y.; Chiba, K. Damage of Embryo Development Caused by Peroxidized Mineral Oil and Its Association with Albumin in Culture. Fertil. Steril. 2009, 91 (5), 1745–1749. https://doi.org/10.1016/j.fertnstert.2008.03.001.
14. Otsuki, J.; Nagai, Y.; Chiba, K. Association of Spindle Midzone Particles with Polo-like Kinase 1 during Meiosis in Mouse and Human Oocytes. Reprod. Biomed. Online 2009, 18 (4), 522–528. https://doi.org/10.1016/s1472-6483(10)60129-0.
15. Hirohashi, N.; Harada, K.; Chiba, K. Hormone-Induced Cortical Maturation Ensures the Slow Block to Polyspermy and Does Not Couple with Meiotic Maturation in Starfish. Dev. Biol. 2008, 318 (1), 194–202. https://doi.org/10.1016/j.ydbio.2008.03.029.
16. Usui, K.; Hirohashi, N.; Chiba, K. Involvement of Mitogen-Activating Protein Kinase and Intracellular pH in the Duration of the Metaphase I (MI) Pause of Starfish Oocytes after Spawning. Dev. Growth Differ. 2008, 50 (5), 357–364. https://doi.org/10.1111/j.1440-169X.2008.01036.x.
17. Otsuki, J.; Nagai, Y.; Chiba, K. Lipofuscin Bodies in Human Oocytes as an Indicator of Oocyte Quality. J. Assist. Reprod. Genet. 2007, 24 (7), 263–270. https://doi.org/10.1007/s10815-007-9130-0.
18. Otsuki, J.; Nagai, Y.; Chiba, K. Peroxidation of Mineral Oil Used in Droplet Culture Is Detrimental to Fertilization and Embryo Development. Fertil. Steril. 2007, 88 (3), 741–743. https://doi.org/10.1016/j.fertnstert.2006.11.144.
19. Sakaue, M.; Motoyama, Y.; Yamamoto, K.; Shiba, T.; Teshima, T.; Chiba, K. Quantitative Measurement of Caspase-3 Activity in a Living Starfish Egg. Biochem. Biophys. Res. Commun. 2006, 350 (4), 878–883. https://doi.org/10.1016/j.bbrc.2006.09.119.
20. Matsuura, R.; Chiba, K. Unequal Cell Division Regulated by the Contents of Germinal Vesicles. Dev. Biol. 2004, 273 (1), 76–86. https://doi.org/10.1016/j.ydbio.2004.04.038.
21. Hyslop, L. A.; Nixon, V. L.; Levasseur, M.; Chapman, F.; Chiba, K.; McDougall, A.; Venables, J. P.; Elliott, D. J.; Jones, K. T. Ca(2+)-Promoted Cyclin B1 Degradation in Mouse Oocytes Requires the Establishment of a Metaphase Arrest. Dev. Biol. 2004, 269 (1), 206–219. https://doi.org/10.1016/j.ydbio.2004.01.030.
22. Oita, E.; Harada, K.; Chiba, K. Degradation of Polyubiquitinated Cyclin B Is Blocked by the MAPK Pathway at the Metaphase I Arrest in Starfish Oocytes. J. Biol. Chem. 2004, 279 (18), 18633–18640. https://doi.org/10.1074/jbc.M311122200.
23. Sasaki, K.; Chiba, K. Induction of Apoptosis in Starfish Eggs Requires Spontaneous Inactivation of MAPK (Extracellular Signal-Regulated Kinase) Followed by Activation of P38MAPK. Mol. Biol. Cell 2004, 15 (3), 1387–1396. https://doi.org/10.1091/mbc.e03-06-0367.
24. Harada, K.; Oita, E.; Chiba, K. Metaphase I Arrest of Starfish Oocytes Induced via the MAP Kinase Pathway Is Released by an Increase of Intracellular pH. Dev. Camb. Engl. 2003, 130 (19), 4581–4586. https://doi.org/10.1242/dev.00649.
25. Iwasaki, H.; Chiba, K.; Uchiyama, T.; Yoshikawa, F.; Suzuki, F.; Ikeda, M.; Furuichi, T.; Mikoshiba, K. Molecular Characterization of the Starfish Inositol 1,4,5-Trisphosphate Receptor and Its Role during Oocyte Maturation and Fertilization. J. Biol. Chem. 2002, 277 (4), 2763–2772. https://doi.org/10.1074/jbc.M108839200.
26. Sasaki, K.; Chiba, K. Fertilization Blocks Apoptosis of Starfish Eggs by Inactivation of the MAP Kinase Pathway. Dev. Biol. 2001, 237 (1), 18–28. https://doi.org/10.1006/dbio.2001.0337.
27. Nezuo, M.; Shogomori, H.; Hoshi, M.; Yamamoto, T.; Teshima, T.; Shiba, T.; Chiba, K. Developmental Changes in Localization of the Main Ganglioside during Sea Urchin Embryogenesis. Glycobiology 2000, 10 (11), 1243–1247. https://doi.org/10.1093/glycob/10.11.1243.
28. Nishigaki, T.; Chiba, K.; Hoshi, M. A 130-KDa Membrane Protein of Sperm Flagella Is the Receptor for Asterosaps, Sperm-Activating Peptides of Starfish Asterias Amurensis. Dev. Biol. 2000, 219 (1), 154–162. https://doi.org/10.1006/dbio.1999.9598.
29. Nakano, T.; Kontani, K.; Kurosu, H.; Katada, T.; Hoshi, M.; Chiba, K. G-Protein Betagamma Subunit-Dependent Phosphorylation of 62-KDa Protein in the Early Signaling Pathway of Starfish Oocyte Maturation Induced by 1-Methyladenine. Dev. Biol. 1999, 209 (1), 200–209. https://doi.org/10.1006/dbio.1999.9248.
30. Chiba, K.; Alderton, J. M.; Hoshi, M.; Steinhardt, R. A. Activation of the Proteasomes of Sand Dollar Eggs at Fertilization Depends on the Intracellular PH Rise. Dev. Biol. 1999, 209 (1), 52–59. https://doi.org/10.1006/dbio.1999.9239.
31. Chiba, K.; Nakano, T.; Hoshi, M. Induction of Germinal Vesicle Breakdown in a Cell-Free Preparation from Starfish Oocytes. Dev. Biol. 1999, 205 (2), 217–223. https://doi.org/10.1006/dbio.1998.9118.
已发表 protocol 1篇
Fluorescence Measurement and Calibration of Intracellular pH in Starfish Oocytes
海星卵母细胞内pH值的荧光测定与校准

作者:Enako Hosoda and Kazuyoshi Chiba日期:10/05/2020,浏览量:370,Q&A: 0
Oocyte maturation is a process wherein an oocyte arrested at prophase of meiosis I resumes meiosis to become a fertilizable egg. In starfish ovaries, a hormone released from follicle cells activates the oocytes, resulting in an increase in their ...