联合作者(
Kohei Suzuki Internal Medicine, Division of Digestive and Liver DIseases University of Texas Southwestern Medical Center
1 protocol

Ryuichi Okamoto
  • Professor, Department of Gastroenterology and Hepatology Tokyo Medical and Dental University (TMDU)
研究方向
  • 干细胞
  • 已发表 protocol 1

教育背景

Graduate School, Graduate School

发表论文

https://scholar.google.co.jp/citations?user=BszbhZMAAAAJ&hl=ja
已发表 protocol 1篇
Fluidigm Based Single-cell Gene Expression Library Preparation from Patient-derived Small Intestinal Organoids
基于flifgm的源于患者小肠类器官单细胞基因表达文库的制备

作者:Kohei Suzuki and Ryuichi Okamoto日期:10/05/2020,浏览量:193,Q&A: 0
In this protocol, we describe our methods to isolate crypts from patients' biopsy samples and to culture human intestinal stem cells as it’s called “organoid.” Beyond that, we describe how to dissociate organoids cells into single cells for ...