联合作者(
Stefan Reber Departement für Chemie und Biochemie, University of Bern, Switzerland, Switzerland,
1 protocol

Jonas Mechtersheimer Basic and Clinical Neuroscience, King's College London, UK, UK,
1 protocol

Marc-David Ruepp
  • Basic and Clinical Neuroscience, King's College London, UK, UK,
研究方向
  • 分子生物学
  • 已发表 protocol 1

Education

Priv.-Doz. (2018) , PhD (2009), University of Bern, Switzerland, 2018

Current position

Senior Lecturer in Neuroscience, Institute of Psychiatry, Psychology & Neuroscience, King's College London, United Kingdom

Junior Group leader, Department of Chemistry and Biochemistry, University of Bern, Switzerland

Publications

  1. https://www.researchgate.net/profile/Marc_David_Ruepp/publications
已发表 protocol 1篇
Generation of Gene Knockout and Gene Replacement with Complete Removal of Full-length Endogenous Transcript Using CRISPR-Trap
通过CRISPR-Trap完全移除内源全长转录本进行基因敲除和基因置换

作者:Jonas Mechtersheimer, Stefan Reber and Marc-David Ruepp日期:10/20/2018,浏览量:5667,Q&A: 0
This protocol describes the application of the CRISPR-Trap from designing of the gene targeting strategy to validation of successfully edited clones that was validated on various human cell lines, among them human induced pluripotent stem cells ...