联合作者(
Douglas Paul Gladue Agricultural Research Service Plum Island Animal Disease Center, Greenport, USA, USA,
2 protocols

Manuel Victor Borca USDA-ARS Orient Point
2 protocols

Elizabeth Ann Vuono USDA-ARS Orient Point
2 protocols

Elizabeth Ramirez-Medina USDA-ARS Orient Point
2 protocols

Keith A Berggren
  • Agricultural Research Service Plum Island Animal Disease Center, Greenport, USA, USA,
研究方向
  • 微生物学
  • 已发表 protocol 1

Education

B.S., University of Connecticut, United States, 2016

Current position

ORISE Research Fellowship, USDA Agricultural Research Service, Plum Island Animal Disease Center, United States

已发表 protocol 1篇
CRISPR/Cas Gene Editing of a Large DNA Virus: African Swine Fever Virus
一种大DNA病毒(非洲猪瘟病毒)的CRISPR/Cas基因编辑

作者:Manuel V. Borca, Keith A. Berggren, Elizabeth Ramirez-Medina, Elizabeth A. Vuono and Douglas P. Gladue日期:08/20/2018,浏览量:6171,Q&A: 0
Gene editing of large DNA viruses, such as African swine fever virus (ASFV), has traditionally relied on homologous recombination of a donor plasmid consisting of a reporter cassette with surrounding homologous viral DNA. However, this homologous ...