联合作者(
L. David Sibley Washington University in St Louis, USA
2 protocols

Shaojun Long Molecular Microbiology, Washington University in St Louis, USA
2 protocols

Kevin M Brown
  • Molecular Microbiology, Washington University in St Louis, USA
研究方向
  • 微生物学
  • 已发表 protocol 2
已发表 protocol 2篇
CRISPR-mediated Tagging with BirA Allows Proximity Labeling in Toxoplasma gondii
可用于刚地弓形虫中邻位标记的CRISPR介导的BirA标记

作者:Shaojun Long, Kevin M. Brown and L. David Sibley日期:03/20/2018,浏览量:8510,Q&A: 0
Defining protein interaction networks can provide key insights into how protein complexes govern complex biological problems. Here we define a method for proximity based labeling using permissive biotin ligase to define protein networks in the ...
Conditional Knockdown of Proteins Using Auxin-inducible Degron (AID) Fusions in Toxoplasma gondii
在刚地弓形虫中通过融合生长素诱导的降解因子(AID)进行蛋白质条件性敲减

作者:Kevin M. Brown, Shaojun Long and L. David Sibley日期:02/20/2018,浏览量:12029,Q&A: 0
Toxoplasma gondii is a member of the deadly phylum of protozoan parasites called Apicomplexa. As a model apicomplexan, there is a great wealth of information regarding T. gondii’s 8,000+ protein coding genes including sequence ...