联合作者(
Eugene I Shakhnovich Department of Chemistry and Chemical Biology, Harvard University, USA
1 protocol

Shimon Bershtein Department of Life Sciences, Ben-Gurion University of the Negev, Israel
1 protocol

Sanchari Bhattacharyya
  • Department of Chemistry and Chemical Biology, Harvard University, USA
  • 已发表 protocol 1
已发表 protocol 1篇
Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters
肠杆菌科中受阿拉伯糖调节启动子调控的基因剂量实验

作者:Sanchari Bhattacharyya, Shimon Bershtein and Eugene I Shakhnovich日期:07/20/2017,浏览量:3414,Q&A: 0
This protocol is used to assay the effect of protein over-expression on fitness of E. coli. It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the ...