联合作者(
Clifford A. Lowell Department of Laboratory Medicine and the Program in Immunology, University of California, USA
1 protocol
联合评审(
Alesssandro Arduini BROAD Institute of Harvard and MIT
2 protocols

Andrea Puhar The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University, Sweden
43 protocols

Antoine de Morree Stanford university
33 protocols

Benoit Chassaing Georgia State University
14 protocols

编委
Meenal Sinha
  • Research scientist, University of California San Francisco
研究方向
  • 免疫学
  • 已发表 protocol 1

教育背景

Ph.D., University of Texas at Houston, 2008

已发表 protocol 1篇
Isolation of Highly Pure Primary Mouse Alveolar Epithelial Type II Cells by Flow Cytometric Cell Sorting
采用流式细胞术分选术分离高纯度原代小鼠II型肺泡上皮细胞

作者:Meenal Sinha and Clifford A. Lowell日期:11/20/2016,浏览量:12150,Q&A: 0
In this protocol, we describe the method for isolating highly pure primary alveolar epithelial type II (ATII) cells from lungs of naïve mice. The method combines negative selection for a variety of lineage markers along with positive selection for ...
参审 protocol 18篇
Induced Germinal Center B Cell Culture System
诱导生发中心B细胞培养系统
作者:Kei Haniuda and Daisuke Kitamura日期:02/20/2019,浏览量:1681,Q&A: 0
The germinal center (GC) is the site where B cells undergo clonal expansion, affinity-based selection, and differentiation into memory B cells or plasma cells. It has been difficult to elucidate regulatory mechanisms for the dynamic GC B cell ...
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Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry
流式细胞术测定TLR4和CD14受体内吞作用
作者:Michael S. Schappe and Bimal N. Desai日期:07/20/2018,浏览量:2736,Q&A: 0
After recognizing extracellular bacterial lipopolysaccharide (LPS), the toll-like receptor 4 (TLR4)-CD14 signaling complex initiates two distinct signaling pathways–one from the plasma membrane and the other from the signaling endosomes (Kagan et ...
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主审 protocol 2篇
Plaque Assay to Determine Invasion and Intercellular Dissemination of Shigella flexneri in TC7 Human Intestinal Epithelial Cells
蚀斑测定弗氏志贺在TC7人肠上皮细胞中的侵袭和细胞间传播
作者:Atin Sharma and Andrea Puhar日期:07/05/2019,浏览量:238,Q&A: 0
Shigella flexneri invades the epithelial cells lining the gut lumen and replicates intracellularly. The specialized Type III Secretion System (T3SS) and its effector proteins, encoded on a large virulence plasmid, assist the bacterium to ...
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Gentamicin Protection Assay to Determine the Number of Intracellular Bacteria during Infection of Human TC7 Intestinal Epithelial Cells by Shigella flexneri
庆大霉素保护试验确定福氏志贺菌侵染人TC7肠上皮细胞的胞内寄生数目
作者:Atin Sharma and Andrea Puhar日期:07/05/2019,浏览量:282,Q&A: 0
Shigella flexneri is an intracellular bacterial pathogen that gains access to the gut epithelium using a specialized Type III Secretion System (T3SS). Various determinants mediating this invasive infection have been experimentally verified ...
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