联合作者(
Clifford A. Lowell Department of Laboratory Medicine and the Program in Immunology, University of California, USA
1 protocol
联合评审(
Alesssandro Arduini BROAD Institute of Harvard and MIT
2 protocols

Alka Mehra Institute of Genomics and Integrative Biology
40 protocols

Andrea Puhar MIMS and Department of Molecular Biology, Umeå University
53 protocols

Anita Umesh Illumina
4 protocols

编委
Meenal Sinha
  • Research scientist, University of California San Francisco
研究方向
  • 免疫学
  • 已发表 protocol 1

教育背景

Ph.D., University of Texas at Houston, 2008

已发表 protocol 1篇
Isolation of Highly Pure Primary Mouse Alveolar Epithelial Type II Cells by Flow Cytometric Cell Sorting
采用流式细胞术分选术分离高纯度原代小鼠II型肺泡上皮细胞

作者:Meenal Sinha and Clifford A. Lowell日期:11/20/2016,浏览量:20552,Q&A: 1
In this protocol, we describe the method for isolating highly pure primary alveolar epithelial type II (ATII) cells from lungs of naïve mice. The method combines negative selection for a variety of lineage markers along with positive selection for ...
参审 protocol 19篇
Isolation of Microglia and Analysis of Protein Expression by Flow Cytometry: Avoiding the Pitfall of Microglia Background Autofluorescence
小胶质细胞的分离及蛋白表达的流式细胞仪分析:避免小胶质细胞背景荧光的陷阱
作者:Jeremy C. Burns, Richard M. Ransohoff and Michaël Mingueneau日期:07/20/2021,浏览量:1494,Q&A: 0

Microglia are a unique type of tissue-resident innate immune cell found within the brain, spinal cord, and retina. In the healthy nervous system, their main functions are to defend the tissue against infectious microbes, support neuronal networks

...
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Induced Germinal Center B Cell Culture System
诱导生发中心B细胞培养系统
作者:Kei Haniuda and Daisuke Kitamura日期:02/20/2019,浏览量:9257,Q&A: 0
The germinal center (GC) is the site where B cells undergo clonal expansion, affinity-based selection, and differentiation into memory B cells or plasma cells. It has been difficult to elucidate regulatory mechanisms for the dynamic GC B cell ...
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主审 protocol 11篇
Flow Cytometry Analysis and Fluorescence-activated Cell Sorting of Myeloid Cells from Lung and Bronchoalveolar Lavage Samples from Mycobacterium tuberculosis-infected Mice
结核分枝杆菌感染小鼠肺和支气管肺泡灌洗样品中髓样细胞的流式细胞仪分析和荧光激活细胞分选
作者:Alissa C. Rothchild, Dat Mai, Alan Aderem and Alan H. Diercks日期:05/20/2020,浏览量:3079,Q&A: 0
Mycobacterium tuberculosis (Mtb) is transmitted by aerosol and can cause serious bacterial infection in the lung that can be fatal if left untreated. Mtb is now the leading cause of death worldwide by an infectious agent. Characterizing ...
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Evaluation of B Cell Proliferation in vivo by EdU Incorporation Assay
通过EdU掺入法评估体内B细胞增殖
作者:Adi Biram and Ziv Shulman日期:05/05/2020,浏览量:2887,Q&A: 0
Generation of antibodies is crucial for establishing enduring protection from invading pathogens, as well as for maintaining homeostasis with commensal bacteria at mucosal surfaces. Chronic exposure to microbiota- and dietary- derived antigens ...
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