联合评审(
Akira Karasawa Molecular Medicine, Cornell University, USA
5 protocols

Alexandros C Kokotos Weill Cornell Medicine
11 protocols

Alka Mehra Institute of Genomics and Integrative Biology
34 protocols

Aruna Bitra La Jolla institute for allergy and immunology
2 protocols

编委
Ralph Thomas Boettcher
  • Project group leader, Max Planck Institute for Biochemistry
研究方向
  • 细胞生物学
  • 已发表 protocol 1
已发表 protocol 1篇
β1 Integrin Cell-surface Immunoprecipitation (Selective Immunoprecipitation)
β1 整合素细胞表面免疫共沉淀(选择性免疫共沉淀)

作者:Ralph T. Böttcher日期:11/05/2013,浏览量:8355,Q&A: 0
Immunoprecipitation (IP) is a widely used method to isolate a specific protein from a mixed protein sample using an antibody that exclusively binds to that particular protein. This technique allows studying protein-protein and protein-nucleic acid ...
参审 protocol 5篇
Isolation of Mononuclear Cell Populations from Ovarian Carcinoma Ascites
从卵巢癌腹水中分离单核细胞群
Ovarian cancer is one of the most fatal tumors in women. Due to a lack of symptoms and adequate screening methods, patients are diagnosed at advanced stages with extensive tumor burden (Jelovac and Armstrong, 2011). Interestingly, ovarian cancer ...
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主审 protocol 41篇
Cell-free Reconstitution of the Packaging of Cargo Proteins into Vesicles at the trans Golgi Network
反面高尔基体网状结构中货物蛋白包装成囊泡的无细胞重组
作者:Xiao Tang, Feng Yang and Yusong Guo日期:03/05/2020,浏览量:529,Q&A: 0
Protein sorting at the trans Golgi network (TGN) plays important roles in targeting newly synthesized proteins to their specific destinations. The aim of this proposal is to reconstitute the packaging of non-Golgi resident cargo proteins ...
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Mesenchymal Stromal Cells Derived from Bone Marrow and Adipose Tissue: Isolation, Culture, Characterization and Differentiation
骨髓和脂肪间充质干细胞:分离、培养、鉴定和分化
Since their discovery, mesenchymal stromal cells (MSCs) have received a lot of attention, mainly due to their self-renewal potential and multilineage differentiation capacity. For these reasons, MSCs are a useful tool in cell biology and ...
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