免疫学

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    现刊
    Phenotyping of Live Human PBMC using CyTOFTM Mass Cytometry
    采用CyTOF质谱流式细胞仪研究人外周血单核细胞表型
    作者:Michael D. Leipold and Holden Maecker日期:01/20/2015,浏览量:10612,Q&A: 3
    [Abstract] Single-cell analysis has become an method of importance in immunology. Fluorescence flow cytometry has been a major player. However, due to issues such as autofluorescence and emission spillover between different fluorophores, alternative techniques are being developed. In recent years, mass cytometry has emerged, wherein antibodies labeled with ...
    Intracellular Cytokine Staining on PBMCs Using CyTOFTM Mass Cytometry
    适用于CyTOF质谱流式细胞仪的外周血单核细胞(PBMC)细胞因子胞内染色方法
    作者:Dongxia Lin, Sheena Gupta and Holden Maecker日期:01/05/2015,浏览量:13910,Q&A: 3
    [Abstract] In this protocol, we use a CyTOFTM mass cytometry to collect single-cell data on a large number of cytokines/chemokines as well as cell-surface proteins that characterize T cells and other immune cells. The current selected mass window in AW 103-203 includes the lanthanides used for most antibody labeling, along with iridium and rhodium ...