免疫检测 —免疫细胞染色 —免疫学

Immunohistochemical Identification of Human Skeletal Muscle Macrophages
人骨骼肌巨噬细胞的免疫组化鉴定

作者：Kate Kosmac, Bailey D. Peck, R. Grace Walton, Jyothi Mula, Philip A. Kern, Marcas M. Bamman, Richard A. Dennis, Cale A. Jacobs, Christian Lattermann, Darren L. Johnson and Charlotte A. Peterson，日期：06/20/2018，浏览量：5080，Q&A： 0

[Abstract] Macrophages have well-characterized roles in skeletal muscle repair and regeneration. Relatively little is known regarding the role of resident macrophages in skeletal muscle homeostasis, extracellular matrix remodeling, growth, metabolism and adaptation to various stimuli including exercise and training. Despite speculation into macrophage ...

Thermal Stability of Heterotrimeric pMHC Proteins as Determined by Circular Dichroism Spectroscopy
采用圆二色光谱法测定异源三聚体pMHC蛋白质的热稳定性

作者：Anna Fuller, Aaron Wall, Michael D Crowther, Angharad Lloyd, Alexei Zhurov, Andrew K. Sewell, David K. Cole and Konrad Beck，日期：07/05/2017，浏览量：4067，Q&A： 0

[Abstract] T cell receptor (TCR) recognition of foreign peptide fragments, presented by peptide major histocompatibility complex (pMHC), governs T-cell mediated protection against pathogens and cancer. Many factors govern T-cell sensitivity, including the affinity of the TCR-pMHC interaction and the stability of pMHC on the surface of antigen presenting ...

Lung Section Staining and Microscopy
肺切片染色和显微镜观察

作者：Xiaofeng Zhou and Bethany B Moore，日期：05/20/2017，浏览量：11770，Q&A： 0

[Abstract] Our protocol describes immunofluorescent staining, hematoxylin and eosin staining and Masson’s trichrome staining on lung sections.

Cell Tracer Violet and CellTracker Red CMTPX Staining of Purified Mature Plasmodium-infected Red Blood Cells
对疟原虫感染的红细胞进行示踪染色以观察免疫吞噬效应

作者：Henrique Borges da Silva, Carlos Eduardo Tadokoro and Maria Regina D’Império Lima，日期：08/05/2016，浏览量：5912，Q&A： 0

[Abstract] Efficient staining methods to identify Plasmodium-infected red blood cells (iRBCs) are crucial to discriminate precisely the immune cells responsible for their elimination from circulation. Here, we describe the protocol for the purification of iRBCs and their subsequent staining with the vital dyes Cell Tracer Violet (CTV) or CellTracker ...

Quantification of Rae-1 Staining Intensity in Glomeruli
肾小球中Rae-1染色强度的测定

作者：Roberto Spada and Domingo F. Barber，日期：03/05/2016，浏览量：4101，Q&A： 0

[Abstract] NKG2D is expressed on all NK cells and on subsets of NKT, CD8, CD4 and γδ T cells. NKG2D is activated by NKG2D ligands, a stress-induced family of MHC-I-like proteins. These ligands are upregulated on stressed/infected cells and are not widely expressed on healthy adult tissue. NKG2D ligands have been widely studied as potential targets for ...

Cytokine-stimulated Phosphoflow of PBMC Using CyTOF Mass Cytometry
CyTOF质谱流式细胞仪测定外周血单核细胞中细胞因子刺激后的磷酸化变化

作者：Rosemary Fernandez and Holden Maecker，日期：06/05/2015，浏览量：10569，Q&A： 1

[Abstract] Phosphorylation of tyrosine, serine, and threonine residues is critical for the control of protein activity involved in various cellular events. An assortment of kinases and phosphatases regulate intracellular protein phosphorylation in many different cell signaling pathways. These pathways include T and B cell signaling, regulating growth and ...

Cytokine-Stimulated Phosphoflow of Whole Blood Using CyTOF Mass Cytometry
CyTOF质谱流式细胞仪测定全血中细胞因子刺激形成的磷酸化变化

作者：Rosemary Fernandez and Holden Maecker，日期：06/05/2015，浏览量：8260，Q&A： 1

[Abstract] The ability to assess the function of a range of cytokine, antigen receptor, and Toll-like receptor (TLR) signaling pathways in a range of immune cells could provide a kind of fingerprint of the state of the human immune system. The mass cytometry or CyTOF, platform allows for the parallel application of about 40 labeled antibodies to a single ...

Phenotyping of Live Human PBMC using CyTOF^TM Mass Cytometry
采用CyTOF质谱流式细胞仪研究人外周血单核细胞表型

作者：Michael D. Leipold and Holden Maecker，日期：01/20/2015，浏览量：10612，Q&A： 3

[Abstract] Single-cell analysis has become an method of importance in immunology. Fluorescence flow cytometry has been a major player. However, due to issues such as autofluorescence and emission spillover between different fluorophores, alternative techniques are being developed. In recent years, mass cytometry has emerged, wherein antibodies labeled with ...

Immunocytochemical Detection of Recombinant Biomphalysin on Schistosoma mansoni Sporocysts
免疫细胞化学法检测曼氏血吸虫孢囊中的重组毒素

作者：David Duval, Richard Galinier, Julien Portela, Guillaume Mitta and Benjamin Gourbal，日期：11/20/2013，浏览量：5861，Q&A： 0

[Abstract] Schistosomiasis, or bilharzia, is a tropical disease caused by worms of the genus Schistosoma which infect about 200 million people. The life cycle of the parasite requires Biomphalaria, a specific genus of freshwater snails, as intermediate. Using an interactome approach employing B. glabrata plasma and S. mansoni ...

Viral Immunofluorescence with Rift Valley Fever Virus Infected MEFs in a 96 Well Plate
在96孔板中用病毒免疫荧光法检测受裂谷热病毒感染的小鼠胚胎成纤维细胞（MEF）

作者：Theresa S. Moser and Sara Cherry，日期：12/05/2012，浏览量：8001，Q&A： 0

[Abstract] Immunofluorescence is a method to detect viral infection in multiple types of host cells. This procedure can be adapted for both high-throughput and low-throughput assays for any virus for which there are antibodies available. Time of infection and virus multiplicity of infection (MOI) vary and should be optimized for each virus and host cell ...

免疫学

Zebrafish Embryo Xenograft and Metastasis Assay

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