哺乳纲

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    现刊
    A mant-GDP Dissociation Assay to Compare the Guanine Nucleotide Binding Preference of Small GTPases
    用Mant-GDP分离实验比较小GTP蛋白的鸟嘌呤核苷酸的结合偏好
    作者:Yuping Tan and Qingxiang Sun日期:01/20/2021,浏览量:110,Q&A: 0
    [Abstract]

    Small GTPases are cellular switches that are switched on when bound to GTP and switched off when bound to GDP. Different small GTPase proteins or those with mutations may bind to GTP or GDP with different relative affinities. However, small GTPases generally have very high affinities for guanine nucleotides, rendering it difficult to compare the

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    Polyamine Transport Assay Using Reconstituted Yeast Membranes
    利用重构酵母细胞膜的多胺转运实验
    作者:Sarah van Veen, Shaun Martin, Marleen Schuermans and Peter Vangheluwe日期:01/20/2021,浏览量:159,Q&A: 0
    [Abstract]

    ATP13A2/PARK9 is a late endo-/lysosomal P5B transport ATPase that is associated with several neurodegenerative disorders. We recently characterized ATP13A2 as a lysosomal polyamine exporter, which sheds light on the molecular identity of the unknown mammalian polyamine transport system. Here, we describe step by step a protocol to measure

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    Imaging of Human Cancer Cells in 3D Collagen Matrices
    人类癌细胞的3D胶原基质成像
    作者:Karin Pfisterer, Brooke Lumicisi and Maddy Parsons日期:01/20/2021,浏览量:139,Q&A: 0
    [Abstract]

    Research on cell migration and interactions with the extracellular matrix (ECM) was mostly focused on 2D surfaces in the past. Many recent studies have highlighted differences in migratory behaviour of cells on 2D surfaces compared to complex cell migration modes in 3D environments. When embedded in 3D matrices, cells constantly sense the

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    Low- and High-resolution Dynamic Analyses for Magnetic Resonance Spectroscopy Data
    磁共振光谱数据的低高分辨率动态分析
    作者:Reuben Rideaux日期:01/20/2021,浏览量:61,Q&A: 0
    [Abstract]

    Magnetic resonance spectroscopy (MRS) can be used to measure in vivo concentrations of neurometabolites. This information can be used to identify neurotransmitter involvement in healthy (e.g., perceptual and cognitive processes) and unhealthy brain function (e.g., neurological and psychiatric illnesses). The standard approach for analyzing MRS

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    Preparation of Synaptoneurosomes to Study the Synapse in the Murine Cerebral Cortex
    用于研究小鼠大脑皮层突触的突触神经小体的制备
    作者:Ariel Diaz, Enrique Torre and Manuel Yepes日期:01/20/2021,浏览量:72,Q&A: 0
    [Abstract]

    The synapse is a complex structure where the transmission of information takes place. Synaptic dysfunction is one of the earliest pathophysiological events in several diseases, such as traumatic brain injury, cerebral ischemia, and neurodegenerative diseases. Thus, a methodology to study synaptic structure and function is crucial for the

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    A Simple Microplate Assay for Reactive Oxygen Species Generation and Rapid Cellular Protein Normalization
    用于活性氧生成和快速细胞蛋白质标准化的简单微孔板检测
    作者:Neville S. Ng and Lezanne Ooi日期:01/05/2021,浏览量:752,Q&A: 0
    [Abstract] 2',7'-dichlorofluorescein (DCF) and derivatives are commonly used as fluorescent indicators of a broad spectrum of reactive oxygen species (ROS) generation in cell-based assays. However, there are numerous challenges inherent to the utilization of DCF probes for intracellular microscopic analysis, including bleaching and probe efflux. Plate ...
    A Transient Transfection-based Cell Adhesion Assay with 293T Cells
    基于瞬时转染的293T细胞粘附实验
    作者:Rohit Singh and Beom K. Choi日期:01/05/2021,浏览量:541,Q&A: 0
    [Abstract]

    The in vitro cell adhesion assay is a quantitative method for measuring selective cell adhesion to specific proteins. Traditionally, cell adhesion assays employ purified protein immobilized on a solid glass or plastic surface. Here, we describe a transient 293T cell transfection-based cell adhesion assay to study selective cell adhesion of a

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    A Parkinson’s Disease-relevant Mitochondrial and Neuronal Morphology High-throughput Screening Assay in LUHMES Cells
    LUHMES细胞中与帕金森氏病相关的线粒体和神经元形态学高通量筛选分析
    作者:Tom Leah, Irina Vazquez-Villaseñor, Laura Ferraiuolo, Stephen B Wharton and Heather Mortiboys日期:01/05/2021,浏览量:473,Q&A: 0
    [Abstract]

    Parkinson’s disease is a devastating neurodegenerative disorder affecting 2-3% of the population over 65 years of age. There is currently no disease-modifying treatment. One of the predominant pathological features of Parkinson’s disease is mitochondrial dysfunction, and much work has aimed to identify therapeutic compounds which can

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    A Quantitative Assay to Measure Stress Granule Association of Proteins and Peptides in Semi-permeabilized Human Cells
    半透性人细胞中蛋白质和肽应激颗粒结合的定量测定
    作者:Saskia Hutten and Dorothee Dormann日期:12/20/2020,浏览量:850,Q&A: 0
    [Abstract]

    Stress granules (SGs) are membrane-less organelles that form in the cytoplasm through phase separation, in response to diverse stressors. SGs contain translationally stalled mRNAs, proteins involved in translation, and various RNA-binding proteins (RBPs). Due to the high local concentration of aggregation-prone RBPs, SGs might act as condensation

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    RNA ImmunoGenic Assay: A Method to Detect Immunogenicity of in vitro Transcribed mRNA in Human Whole Blood
    RNA ImmunoGenic分析:一种检测人全血体外转录mRNA免疫原性的方法
    [Abstract] The mRNA therapeutics is a new class of medicine to treat many various diseases. However, in vitro transcribed (IVT) mRNA triggers immune responses due to recognition by human endosomal and cytoplasmic RNA sensors, but incorporation of modified nucleosides have been shown to reduce such responses. Therefore, an assay signifying important ...