哺乳纲

分类

    现刊
    Determination of Cellular Uptake and Endocytic Pathways
    细胞摄取和内吞途径的检测
    作者:Jing Gong, Hong-Xia Wang and Kam W. Leong日期:02/20/2019,浏览量:98,Q&A: 0
    [Abstract] Efficiency of drug and gene delivery via nonviral vehicles is contingent on proper cellular uptake and intracellular release. Further, various cargos, such as nucleases for gene editing or inhibitors for endosomal receptors, require transport to specific compartments of the cell. Hence, characterization of cellular uptake and endocytic pathways is ...
    On-demand Labeling of SNAP-tagged Viral Protein for Pulse-Chase Imaging, Quench-Pulse-Chase Imaging, and Nanoscopy-based Inspection of Cell Lysates
    用于脉冲追踪成像,猝发脉冲追踪成像和基于纳米显微技术的细胞裂解物检测的融合SNAP病毒蛋白的按需标记
    作者:Roland Remenyi, Raymond Li and Mark Harris日期:02/20/2019,浏览量:52,Q&A: 0
    [Abstract] Advanced labeling technologies allow researchers to study protein turnover inside intact cells and to track the labeled protein in downstream applications. In the context of a viral infection, the combination of imaging and fluorescent labeling of viral proteins sheds light on their biological activity and interaction with the host cell. Initial ...
    Isolation of Multipotent Mesenchymal Stem Cells from Human Extraocular Muscle Tissue
    人眼外肌组织多能间充质干细胞的分离
    作者:Amit Sharma, Darilang Mawrie, Damarais Magdalene and Bithiah Grace Jaganathan日期:02/20/2019,浏览量:75,Q&A: 0
    [Abstract] Mesenchymal stem cells (MSCs) have attracted significant attention as potential therapeutic cells to treat various diseases ranging from tissue injuries, graft versus host disease, degenerative diseases and cancer. Since the initial discovery of MSCs in the bone marrow cells, MSCs have been successfully isolated from various adult and neo-natal ...
    Imaging Higher-order Chromatin Structures in Single Cells Using Stochastic Optical Reconstruction Microscopy
    随机光学重建显微镜用于单细胞中高级染色体结构的成像
    作者:Jianquan Xu and Yang Liu日期:02/05/2019,浏览量:310,Q&A: 0
    [Abstract] Higher-order chromatin organization shaped by epigenetic modifications influence the chromatin environment and subsequently regulate gene expression. Direct visualization of the higher-order chromatin structure at their epigenomic states is of great importance for understanding chromatin compaction and its subsequent effect on gene expression and ...
    Measurement of Redox States of the β3 Integrin Disulfide Bonds by Mass Spectrometry
    β3整合素二硫键氧化还原态的质谱检测
    作者:Joyce Chiu日期:02/05/2019,浏览量:238,Q&A: 0
    [Abstract] Functional disulfide bonds mediate a change in protein function in which they reside when cleaved or formed. To elucidate how a functional disulfide bond controls protein activity, it is critical that the redox state of the bond in the population of protein molecules is known. Measurement of changes in disulfide bond redox state relies on thiol ...
    Immunohistochemical Staining of TLR4 in Human Skeletal Muscle Samples
    人类骨骼肌样本中TLR4的免疫组化染色
    作者:Daniela Verzola, Samantha Milanesi, Francesca Costigliolo and Giacomo Garibotto日期:01/20/2019,浏览量:372,Q&A: 0
    [Abstract] Growing evidence suggests the involvement of TLR4, a receptor in the innate immune system, in muscle loss in uremia. Recently, we have evaluated TLR4 in human skeletal muscle from chronic kidney disease patients, by immunohistochemistry and image analysis. Unlike the commonly-used Western blot method, immunohistochemistry allows for the ...
    Assessment of Humoral Alloimmunity in Mixed Lymphocyte Reaction
    混合淋巴细胞反应中体液同种异体免疫的评估
    作者:Georgios Pissas and Theodoros Eleftheriadis日期:01/20/2019,浏览量:442,Q&A: 0
    [Abstract] Humoral alloimmunity remains a significant and unresolved problem that constrains allograft survival. Thus, there is a need for the development of an easy, preferably non-radioactive, and inexpensive protocol for assessing the effect of various drug treatments on humoral alloimmunity. In order to satisfy this demand, we developed such a protocol ...
    Detection of Ligand-binding to Membrane Proteins by Capacitance Measurements
    通过电容测量来测定膜蛋白的配体结合
    作者:Verena Burtscher, Matej Hotka and Walter Sandtner日期:01/05/2019,浏览量:499,Q&A: 0
    [Abstract] In multi-cellular organisms, cells communicate with each other utilizing chemical messengers. For many of these messenger molecules, the membrane is an insurmountable barrier. Yet, they act by binding to surface proteins often triggering a cascade of reactions inside the cell. Accordingly, studying ligand-receptor interactions at the cellular ...
    Evaluation of Anticancer activity of Silver Nanoparticles on the A549 Human Lung Carcinoma Cell Lines through Alamar Blue Assay
    通过阿尔玛蓝显色法评估A549人肺癌细胞系上的银纳米粒子
    作者:Nikita Sharma, Geeta Arya, R. Mankamna Kumari, Nidhi Gupta and Surendra Nimesh日期:01/05/2019,浏览量:536,Q&A: 0
    [Abstract] Silver nanoparticles have been widely studied to possess antimicrobial as well as anticancer activity, and have found its applications in various fields including pharmaceutical industry, diagnostics, drug delivery, food industry, and others. For this purpose, several cell proliferation assays are widely used for the evaluation of anticancer ...
    Monitoring Natural Killer Cell Function in Human Ovarian Cancer Cells of Ascitic Fluid
    在人卵巢腹水癌细胞中监测自然杀手细胞功能
    作者:Vikash Kumar and Asok Mukhopadhyay日期:12/20/2018,浏览量:442,Q&A: 0
    [Abstract] Natural killer (NK) cells are the major effectors of the innate immune system when activated resulting in modulation of immune response of the host defense through target cell lysis and secretion of cytokines. Precise functions of NK cells are essential for the treatment outcome of different virus infections and malignant diseases. NK cells impart ...