免疫细胞染色 —免疫学

Flow Cytometry Assay for Recycling of LFA-1 in T-lymphocytes
流式细胞术检测T淋巴细胞中LFA-1的再循环

作者：Katarzyna Potrzebowska, Janne Lehtonen, Malin Samuelsson and Lena Svensson，日期：12/05/2018，浏览量：506，Q&A： 0

[Abstract] To enable cells to move forward, cell surface integrins are internalized into an endosomal compartment and subsequently intracellularly transported to be re-exposed at a new site on the cell membrane. Leukocytes are the fastest migrating cell type in the human body, which express the leukocyte-specific integrin LFA-1. Here, we describe a flow ...

Identification and Quantitation of Leukocyte Populations in Human Kidney Tissue by Multi-parameter Flow Cytometry
利用多参数流式细胞技术对人肾脏组织中白细胞种群的鉴定与定量分析

作者：Katrina Kildey, Becker M.P. Law, Kimberly A. Muczynski, Ray Wilkinson, Helen Healy and Andrew J. Kassianos，日期：08/20/2018，浏览量：897，Q&A： 0

[Abstract] Inflammatory immune cells play direct pathological roles in cases of acute kidney injury (AKI) and chronic kidney disease (CKD). However, the identification and characterization of distinct populations of leukocytes in human kidney biopsies have been confounded by the limitations of immunohistochemical (IHC)-based techniques used to detect them. ...

Immunohistochemical Identification of Human Skeletal Muscle Macrophages
人骨骼肌巨噬细胞的免疫组化鉴定

作者：Kate Kosmac, Bailey D. Peck, R. Grace Walton, Jyothi Mula, Philip A. Kern, Marcas M. Bamman, Richard A. Dennis, Cale A. Jacobs, Christian Lattermann, Darren L. Johnson and Charlotte A. Peterson，日期：06/20/2018，浏览量：2762，Q&A： 0

[Abstract] Macrophages have well-characterized roles in skeletal muscle repair and regeneration. Relatively little is known regarding the role of resident macrophages in skeletal muscle homeostasis, extracellular matrix remodeling, growth, metabolism and adaptation to various stimuli including exercise and training. Despite speculation into macrophage ...

Visualization of RNA at the Single Cell Level by Fluorescent in situ Hybridization Coupled to Flow Cytometry
采用荧光原位杂交结合流式细胞术在单细胞水平上观察RNA

作者：Alice Coillard and Elodie Segura，日期：06/20/2018，浏览量：1587，Q&A： 0

[Abstract] The protocol described here has been developed to detect RNA at the single cell level. Fluorescent probes hybridize to target RNAs and are detected by flow cytometry after multiple amplification steps. Different types of RNA can be detected such as mRNA, long noncoding RNA, viral RNA or telomere RNA and up to 4 different target probes can be used ...

Analysing Temporal Dynamics of T Cell Division in vivo Using Ki67 and BrdU Co-labelling by Flow Cytometry
使用Ki67和BrdU共同标记通过流式细胞术分析体内T细胞分裂的时间动力学

作者：Thea Hogan, Andrew Yates and Benedict Seddon，日期：12/20/2017，浏览量：3481，Q&A： 0

[Abstract] This protocol was developed to increase the richness of information available from in vivo T cell proliferation studies. DNA labelling techniques such as BrdU incorporation allow precise control of label administration and withdrawal, so that the division history of a population can be tracked in detail over long timeframes (days-weeks). ...

Thermal Stability of Heterotrimeric pMHC Proteins as Determined by Circular Dichroism Spectroscopy
采用圆二色光谱法测定异源三聚体pMHC蛋白质的热稳定性

作者：Anna Fuller, Aaron Wall, Michael D Crowther, Angharad Lloyd, Alexei Zhurov, Andrew K. Sewell, David K. Cole and Konrad Beck，日期：07/05/2017，浏览量：3162，Q&A： 0

[Abstract] T cell receptor (TCR) recognition of foreign peptide fragments, presented by peptide major histocompatibility complex (pMHC), governs T-cell mediated protection against pathogens and cancer. Many factors govern T-cell sensitivity, including the affinity of the TCR-pMHC interaction and the stability of pMHC on the surface of antigen presenting ...

Lung Section Staining and Microscopy
肺切片染色和显微镜观察

作者：Xiaofeng Zhou and Bethany B Moore，日期：05/20/2017，浏览量：8739，Q&A： 2

[Abstract] Our protocol describes immunofluorescent staining, hematoxylin and eosin staining and Masson’s trichrome staining on lung sections.

MHC Class II Tetramer Labeling of Human Primary CD4⁺ T Cells from HIV Infected Patients
使用MHC II类四聚体标记来自HIV感染患者的人原代CD4+ T细胞

作者：Moran Galperin, Daniela Benati, Mathieu Claireaux, Madhura Mukhopadhyay and Lisa A. Chakrabarti，日期：03/20/2017，浏览量：4124，Q&A： 0

[Abstract] Major Histocompatibility Complex (MHC) tetramers have been used for two decades to detect, isolate and characterize T cells specific for various pathogens and tumor antigens. In the context of Human Immunodeficiency Virus (HIV) infection, antigen-specific CD8⁺ T cells have been extensively studied ex vivo, as they can be ...

Isolation of Highly Pure Primary Mouse Alveolar Epithelial Type II Cells by Flow Cytometric Cell Sorting
采用流式细胞术分选术分离高纯度原代小鼠II型肺泡上皮细胞

作者：Meenal Sinha and Clifford A. Lowell，日期：11/20/2016，浏览量：10238，Q&A： 0

[Abstract] In this protocol, we describe the method for isolating highly pure primary alveolar epithelial type II (ATII) cells from lungs of naïve mice. The method combines negative selection for a variety of lineage markers along with positive selection for EpCAM, a pan-epithelial cell marker. This method yields 2-3 x 10⁶ ATII cells per mouse ...

Flow Cytometry of Lung and Bronchoalveolar Lavage Fluid Cells from Mice Challenged with Fluorescent Aspergillus Reporter (FLARE) Conidia
采用流式细胞仪检测被带有荧光报告基因的曲霉（FLARE）分生孢子感染过的小鼠肺部和支气管肺泡灌洗液细胞

作者：Anupam Jhingran, Shinji Kasahara and Tobias M Hohl，日期：09/20/2016，浏览量：6875，Q&A： 0

[Abstract] Aspergillus fumigatus is a ubiquitous fungal pathogen that forms airborne conidia. The process of restricting conidial germination into hyphae by lung leukocytes is critical in determining infectious outcomes. Tracking the outcome of conidia-host cell encounters in vivo is technically challenging and an obstacle to understanding ...

免疫学

Zebrafish Embryo Xenograft and Metastasis Assay

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