Skin Transplantation and Lymphoid Organ Analysis in Mice
小鼠中皮肤移植和淋巴器官分析
[Abstract] Skin transplantation in mice is an important procedure to evaluate immune responses generated against heterologous grafts, especially given its highly immunogenic nature. In fact, skin is one of the most challenging organs in terms of allograft retention. In this protocol, we provide a detailed procedure for skin grafting using the tail skin as ...
Immunohistochemical Staining of CD8α in Diabetic Mouse Kidney
糖尿病小鼠肾脏CD8α的免疫组化染色
[Abstract] Immune cell infiltration, particularly cytotoxic CD8α lymphocyte infiltration, plays an important role in development of diabetic nephropathy. Although CD8α infiltration can be evaluated by its production of cytokines, its localization in the kidney is of particular importance. The current protocol describes CD8α immunostaining using a Vectastain ...
In vitro Infection of Primary Human Monocytes with HIV-1
原代人单核细胞的体外HIV-1侵染
[Abstract] Monocyte infection by HIV-1 is an important component of chronic HIV pathogenesis. Following infection by HIV-1, monocytes are able to cross the blood brain barrier and set up a viral reservoir in the central nervous system. Additionally, in the setting of chronic HIV-1 infection, monocytes can become activated either directly through HIV-1 ...
Flow Cytometry Assay for Recycling of LFA-1 in T-lymphocytes
流式细胞术检测T淋巴细胞中LFA-1的再循环
[Abstract] To enable cells to move forward, cell surface integrins are internalized into an endosomal compartment and subsequently intracellularly transported to be re-exposed at a new site on the cell membrane. Leukocytes are the fastest migrating cell type in the human body, which express the leukocyte-specific integrin LFA-1. Here, we describe a flow ...
Immunohistochemical Identification of Human Skeletal Muscle Macrophages
人骨骼肌巨噬细胞的免疫组化鉴定
[Abstract] Macrophages have well-characterized roles in skeletal muscle repair and regeneration. Relatively little is known regarding the role of resident macrophages in skeletal muscle homeostasis, extracellular matrix remodeling, growth, metabolism and adaptation to various stimuli including exercise and training. Despite speculation into macrophage ...