单细胞分析 —细胞生物学

High Dimensional Functionomic Analysis of Human Hematopoietic Stem and Progenitor Cells at a Single Cell Level
人造血干细胞和祖细胞在单细胞水平上的高维功能组学分析

作者：Thomas Luh, Kimberly Lucero, Wenji Ma, Jaeyop Lee, Yu Jerry Zhou, Yufeng Shen and Kang Liu，日期：05/20/2018，浏览量：1642，Q&A： 0

[Abstract] The ability to conduct investigation of cellular transcription, signaling, and function at the single-cell level has opened opportunities to examine heterogeneous populations at unprecedented resolutions. Although methods have been developed to evaluate high-dimensional transcriptomic and proteomic data (relating to cellular mRNA and protein), ...

Determining Genome Size from Spores of Seedless Vascular Plants
无种子维管束植物孢子基因组大小的测定

作者：Li-Yaung Kuo and Yao-Moan Huang ，日期：06/05/2017，浏览量：3520，Q&A： 0

[Abstract] Seedless vascular plants, including ferns and lycophytes, produce spores to initiate the gametophyte stage and to complete sexual reproduction. Approximately 10% of them are apomictic through the production of genomic unreduced spores. Being able to measure the spore nuclear DNA content is therefore important to infer their reproduction mode. Here ...

Flow Cytometric Analysis of HIV-1 Transcriptional Activity in Response to shRNA Knockdown in A2 and A72 J-Lat Cell Lines
A2和A72 J-Lat细胞系中HIV-1经shRNA沉默后转录活性的流式细胞分析

作者：Daniela Boehm and Melanie Ott，日期：06/05/2017，浏览量：3720，Q&A： 0

[Abstract] The main obstacle to eradicating HIV-1 from patients is post-integration latency (Finzi et al., 1999). Antiretroviral treatments target only actively replicating virus, while latent infections that have low or no transcriptional activity remain untreated (Sedaghat et al., 2007). To eliminate viral reservoirs, one strategy focuses ...

Flow Cytometric Analysis of Drug-induced HIV-1 Transcriptional Activity in A2 and A72 J-Lat Cell Lines
A2和A72 J-Lat细胞系中药物诱导的HIV-1转录活性的流式细胞术分析

作者：Daniela Boehm and Melanie Ott，日期：05/20/2017，浏览量：4718，Q&A： 5

[Abstract] The main obstacle to eradicating HIV-1 from patients is post-integration latency (Finzi et al., 1999). Antiretroviral treatments target only actively replicating virus, while latent infections that have low or no transcriptional activity remain untreated (Sedaghat et al., 2007). A combination of antiretroviral treatments with ...

Quantitative Analysis of Exosome Secretion Rates of Single Cells
单细胞外泌体分泌速率的定量分析

作者：Yu-Jui Chiu, Wei Cai, Tiffany Lee, Julia Kraimer and Yu-Hwa Lo，日期：02/20/2017，浏览量：5605，Q&A： 0

[Abstract] To study the inhomogeneity within a cell population including exosomes properties such as exosome secretion rate of cells and surface markers carried by exosomes, we need to quantify and characterize those exosomes secreted by each individual cell. Here we develop a method to collect and analyze exosomes secreted by an array of single cells using ...

Protocol for Microfluidic System to Automate the Preparation and Fractionation of the Nucleic Acids in the Cytoplasm Versus Nuclei of Single Cells
自动制备和分级分离单细胞细胞质与细胞核中核酸的微流体系统方法

作者：Kentaro Kuriyama, Hirofumi Shintaku and Juan G. Santiago，日期：06/20/2016，浏览量：3821，Q&A： 0

[Abstract] This protocol describes the extraction, fractionation, and recovery of cytoplasmic nucleic acids (e.g., cytoplasmic RNA) versus nucleic acids in the cell nucleus (including genomic DNA, gDNA) from single cells with a microfluidic system. The method enables independent, sequence-specific analyses of these critical markers (Kuriyama et al. ...

Phenotyping of Live Human PBMC using CyTOF^TM Mass Cytometry
采用CyTOF质谱流式细胞仪研究人外周血单核细胞表型

作者：Michael D. Leipold and Holden Maecker，日期：01/20/2015，浏览量：8909，Q&A： 3

[Abstract] Single-cell analysis has become an method of importance in immunology. Fluorescence flow cytometry has been a major player. However, due to issues such as autofluorescence and emission spillover between different fluorophores, alternative techniques are being developed. In recent years, mass cytometry has emerged, wherein antibodies labeled with ...

Adenosine A_2A Receptor Ligand Binding Experiments by Using Real-time Single-cell FRET
采用实时单细胞荧光共振能量转移(FREET)法进行腺苷A2A受体配体结合实验

作者：Víctor Fernández-Dueñas, Kenneth A. Jacobson and Francisco Ciruela，日期：03/20/2014，浏览量：6362，Q&A： 0

[Abstract] We designed a fluorescence resonance energy transfer (FRET)-based approach to study the ligand binding constants of the adenosine A_2A receptor (A_2AR). Our assay is based in the interaction of a fluorescent A_2AR agonist ligand (MRS5424) with an A_2AR tagged with the cyan fluorescent protein (CFP) at the ...

Neutral Comet Assay
中性单细胞凝胶电泳分析

作者：Elisa Boutet-Robinet, Didier Trouche and Yvan Canitrot，日期：09/20/2013，浏览量：17840，Q&A： 1

[Abstract] The Comet assay (or Single Cell Gel Electrophoresis assay) is a sensitive technique to detect DNA damage at the level of an individual cell. This technique is based on micro-electrophoresis of cells DNA content. Briefly, cells are embedded in agarose, lysed and submitted to an electric field, before the staining step with a fluorescent DNA binding ...

Modified Single-Cell Transient Gene Expression Assay in Barley Epidermal Cells
大麦表皮细胞中一种改良的单细胞转基因技术-粒子枪法

作者：Shiwei Bai, Cheng Chang, Xinyun Han and Qian-Hua Shen，日期：05/05/2013，浏览量：6120，Q&A： 0

[Abstract] Transient gene expression via biolistic particle delivery is a widely used technique for gene functional analysis in plants. In this protocol we describe a modified single-cell transient expression assay through transformation with a particle inflow gun of the model PDS-1000/He system (Bio-Rad). This assay was originally optimized for analyzing ...

细胞生物学

Zebrafish Embryo Xenograft and Metastasis Assay

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