干细胞

分类

    现刊
    One-step Derivation of Functional Mesenchymal Stem Cells from Human Pluripotent Stem Cells
    人多能干细胞到功能性间充质干细胞的单步派生
    作者:Yan Zhou, JinQi Liao, ChongZhou Fang, CuiPing Mo, GuangQian Zhou and Yonglun Luo日期:11/20/2018,浏览量:231,Q&A: 0
    [Abstract] Mesenchymal stem cells (MSCs) are invaluable cell sources for understanding stem cell biology and potential application in tissue engineering and regenerative medicine. The current issues of MSCs that demand to be further addressed are limited donors, tissue sources and limited capacity of ex vivo expansion. Here, we describe a simple and ...
    Generation of BMEC Lines and in vitro BMEC-HSPC Co-culture Assays
    建立脑微血管内皮细胞系和体外 BMEC-HSPC 共培养检测
    作者:Peipei Guo and Shahin Rafii日期:11/05/2018,浏览量:202,Q&A: 0
    [Abstract] Endothelial cells (ECs) sustain the self-renewal and regeneration of adult hematopoietic stem and progenitor cells (HSPCs) via deployment of EC-derived paracrine factors, termed as angiocrine factors. Generation of durable ex vivo vascular niche that maintains EC identity and preserves the angiocrine profile of organ of origin offers ...
    mRNA Stability Assay Using Transcription Inhibition by Actinomycin D in Mouse Pluripotent Stem Cells
    利用放线菌素D抑制转录检测小鼠多能干细胞中mRNA的稳定性
    作者:Madara Ratnadiwakara and Minna-Liisa Änkö日期:11/05/2018,浏览量:220,Q&A: 0
    [Abstract] Gene expression is regulated through multiple steps at both transcriptional and post-transcriptional levels. The net abundance of mature mRNA species in cells is determined by the balance between transcription and degradation. Thus, the regulation of mRNA stability is a key post-transcriptional event that can greatly affect the net level of mRNAs ...
    RNA Immunoprecipitation Assay to Determine the Specificity of SRSF3 Binding to Nanog mRNA
    利用RNA免疫沉淀试验确定SRSF3结合Nanog mRNA的特异性
    作者:Madara Ratnadiwakara and Minna-Liisa Änko日期:11/05/2018,浏览量:220,Q&A: 0
    [Abstract] Mammalian cells express hundreds of RNA binding proteins (RBPs) that are essential regulators of RNA metabolism. RBP activity plays a central role in the control of gene expression programs and identification of RNA-protein interactions is critical for comprehensive understanding of gene regulation in cells. In recent years, various tools and ...
    Maintenance of Schmidtea mediterranea in the Laboratory
    实验室真涡虫的保存
    作者:Nídia de Sousa and Teresa Adell日期:10/05/2018,浏览量:268,Q&A: 0
    [Abstract] In the last years, planarians have emerged as a unique model animal for studying regeneration and stem cells biology. Although their remarkable regenerative abilities are known for a long time, only recently the molecular tools to understand the biology of planarian stem cells and the fundamentals of their regenerative process have been ...
    Isolation of Chromatin-bound Proteins from Subcellular Fractions for Biochemical Analysis
    从亚细胞成分中分离用于生化分析的染色质结合蛋白
    作者:Sébastien Gillotin日期:10/05/2018,浏览量:451,Q&A: 0
    [Abstract] Shuttling of proteins between different cellular compartments controls their proteostasis and can contribute in some cases to regulate their activity. Biochemical analysis of chromatin-bound proteins, such as transcription factors, is often difficult because of their low yield and due to the interference from nucleic acids. This protocol describes ...
    Qualitative in vivo Bioluminescence Imaging
    体内定性生物发光成像技术
    作者:Devbarna Sinha, Zalitha Pieterse and Pritinder Kaur日期:09/20/2018,浏览量:607,Q&A: 0
    [Abstract] Bioluminescence imaging (BLI) technology is an advanced method of carrying out molecular imaging on live laboratory animals in vivo. This powerful technique is widely-used in studying a variety of biological processes, and it has been an ideal tool in exploring tumor growth and metastatic spread in real-time. This technique ensures the ...
    Microarray, IPA and GSEA Analysis in Mice Models
    关于小鼠模型的微阵列,IPA和GSEA分析
    作者:Stephanie N. Oprescu, Katharine A. Horzmann, Feng Yue, Jennifer L. Freeman and Shihuan Kuang日期:09/05/2018,浏览量:581,Q&A: 0
    [Abstract] This protocol details a method to analyze two tissue samples at the transcriptomic level using microarray analysis, ingenuity pathway analysis (IPA) and gene set enrichment analysis (GSEA). Methods such as these provide insight into the mechanisms underlying biological differences across two samples and thus can be applied to interrogate a variety ...
    Investigating Neural Stem Cell and Glioma Stem Cell Self-renewal Potential Using Extreme Limiting Dilution Analysis (ELDA)
    利用极端有限稀释分析法(ELDA)检测神经干细胞和胶质瘤干细胞的自我更新能力
    作者:Hong PT Nguyen, Paul M Daniel, Gulay Filiz and Theo Mantamadiotis日期:09/05/2018,浏览量:550,Q&A: 0
    [Abstract] Glioma stem cells (GSC) grown as neurospheres exhibit similar characteristics to neural stem cells (NSC) grown as neurospheres, including the ability to self-renew and differentiate. GSCs are thought to play a role in cancer initiation and progression. Self-renewal potential of GSCs is thought to reflect many characteristics associated with ...
    Generation of Human Mesenchymal Stem Cell 3D Spheroids Using Low-binding Plates
    利用低结合培养板培养产生人间充质干细胞3D球状体
    [Abstract] The 3D culture of human mesenchymal stem cells (hMSCs) represents a more physiological environment than classical 2D culture and has been used to enhance the MSC secretome or extend cell survival after transplantation. Here we describe a simple and affordable method to generate 3D spheroids of hMSCs by seeding them at high density in a low-binding ...