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干细胞

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    现刊
    FACS-based Isolation of Neural and Glioma Stem Cell Populations from Fresh Human Tissues Utilizing EGF Ligand
    基于FACS利用EGF配体从新鲜人组织中分离神经和胶质瘤干细胞群
    作者:Jessica Tome-Garcia, Fiona Doetsch and Nadejda M. Tsankova日期:12/20/2017,浏览量:522,Q&A: 0
    [Abstract] Direct isolation of human neural and glioma stem cells from fresh tissues permits their biological study without prior culture and may capture novel aspects of their molecular phenotype in their native state. Recently, we demonstrated the ability to prospectively isolate stem cell populations from fresh human germinal matrix and glioblastoma ...
    GC/MS-based Analysis of Volatile Metabolic Profile Along in vitro Differentiation of Human Induced Pluripotent Stem Cells
    基于GC/MS的人诱导多能干细胞体外分化过程中挥发性化合物代谢谱分析
    [Abstract] Human induced pluripotent stem cells (hiPSCs) are a promising tool in cell-based therapies for degenerative diseases. A safe application of hiPSCs in vivo, requires the detection of the presence of residual undifferentiated pluripotent cells that can potentially cause the insurgence of teratomas. Several studies point out that metabolic ...
    Obtaining Multi-electrode Array Recordings from Human Induced Pluripotent Stem Cell–Derived Neurons
    从人诱导性多能干细胞神经元获得多电极阵列记录
    作者:Xiaohong Xu, Carola I. Radulescu, Kagistia Hana Utami and Mahmoud A. Pouladi 日期:11/20/2017,浏览量:663,Q&A: 0
    [Abstract] Neuronal electrical properties are often aberrant in neurological disorders. Human induced pluripotent stem cells (hiPSCs)-derived neurons represent a useful platform for neurological disease modeling, drug discovery and toxicity screening in vitro. Multi-electrode array (MEA) systems offer a non-invasive and label-free platform to record ...
    Isolation of Primary Human Skeletal Muscle Cells
    原代人骨骼肌细胞的分离
    作者:Janelle M. Spinazzola and Emanuela Gussoni日期:11/05/2017,浏览量:576,Q&A: 0
    [Abstract] Primary myoblast culture is a valuable tool in research of muscle disease, pathophysiology, and pharmacology. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic population by fluorescence-activated cell sorting (FACS). We also describe methods for assessing ...
    Isolation, Culture and Differentiation of Adult Hippocampal Precursor Cells
    成年海马前体细胞的分离,培养和分化
    作者:Stefanie N. Bernas, Odette Leiter, Tara L. Walker and Gerd Kempermann日期:11/05/2017,浏览量:952,Q&A: 0
    [Abstract] There are two neurogenic niches in the adult mammalian brain: the subventricular zone of the lateral ventricle and the subgranular zone of the hippocampal dentate gyrus. Cells from these areas can be isolated and maintained in vitro, using two different culture systems to assess their potential regarding proliferation and differentiation ...
    In vitro Co-culture of Mesenchymal Stem Cells and Endothelial Colony Forming Cells
    间充质干细胞和内皮集落形成细胞的体外共培养
    作者:Abbas Shafiee and Kiarash Khosrotehrani日期:10/20/2017,浏览量:1090,Q&A: 0
    [Abstract] The discovery of endothelial colony forming cells (ECFCs) with robust self-renewal and de novo vessel formation potentials suggests that ECFCs can be an excellent cell source for cardiovascular diseases treatment through improving neovascularization in the ischemic tissues. However, their engraftment after transplantation resulted to be low. ...
    Teratoma Formation Assay for Assessing Pluripotency and Tumorigenicity of Pluripotent Stem Cells
    采用畸胎瘤形成分析法评估多能干细胞的多能性和致瘤性
    作者:Shingo Miyawaki, Yohei Okada, Hideyuki Okano and Kyoko Miura日期:08/20/2017,浏览量:1489,Q&A: 0
    [Abstract] Pluripotent stem cells such as induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) form teratomas when transplanted into immunodeficient mice. As teratomas contain all three germ layers (endoderm, mesoderm, ectoderm), teratoma formation assay is widely used as an index of pluripotency (Evans and Kaufman, 1981; Hentze et al. ...
    Exit from Pluripotency Assay of Mouse Embryonic Stem Cells
    小鼠胚胎干细胞退出多能性测定实验
    作者:Daniel Cirera-Salinas and Constance Ciaudo日期:08/20/2017,浏览量:1150,Q&A: 0
    [Abstract] A novel method to assess the dissolution of the core pluripotency transcription-factor circuit of mouse Embryonic Stem Cells (mESCs) has been developed (Ying et al., 2003; Betschinger et al., 2013). In order to efficiently identify genes essential for the break-down of the pluripotency network in mutant mESCs with proliferation ...
    A Co-culture Assay to Determine Efficacy of TNF-α Suppression by Biomechanically Induced Human Bone Marrow Mesenchymal Stem Cells
    人骨髓间充质干细胞共培养实验测定生物力学诱导的TNF-α抑制功效
    作者:Miguel F. Diaz, Siobahn M. Evans, Scott D. Olson, Charles S. Cox Jr and Pamela L. Wenzel日期:08/20/2017,浏览量:1113,Q&A: 0
    [Abstract] The beneficial effects of mesenchymal stem cell (MSC)-based cellular therapies are believed to be mediated primarily by the ability of MSCs to suppress inflammation associated with chronic or acute injury, infection, autoimmunity, and graft-versus-host disease. To specifically address the effects of frictional force caused by blood flow, or wall ...
    Isolation and Expansion of Mesenchymal Stem Cells from Murine Adipose Tissue
    从鼠脂肪组织中分离和扩增间充质干细胞
    作者:Natalia V. Andreeva, Alexandra A. Dalina and Alexander V. Belyavsky日期:08/20/2017,浏览量:1108,Q&A: 0
    [Abstract] Mesenchymal stem cells (MSCs) are currently intensively studied due to significant promise which they represent for successful implementations of future cell therapy clinical protocols. This in turn emphasizes importance of careful preclinical studies of MSC effects in various murine disease models. The appropriate cell preparations with ...