鱼类

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    现刊
    Notochord Injury Assays that Stimulate Transcriptional Responses in Zebrafish Larvae
    诱导幼年期斑马鱼转录响应的脊索损伤实验
    Zebrafish have become an increasingly important model organism in the field of wound healing and regenerative medicine, due to their high regenerative capacity coupled with high-resolution imaging in living animals. In a recent study, we described ...
    Notochord Injury Assays that Stimulate Transcriptional Responses in Zebrafish Larvae
    诱导幼年期斑马鱼转录响应的脊索损伤实验
    [Abstract] Zebrafish have become an increasingly important model organism in the field of wound healing and regenerative medicine, due to their high regenerative capacity coupled with high-resolution imaging in living animals. In a recent study, we described multiple physical and chemical methods to induce notochord injury that led to highly specific ...
    Mosaic Labeling and 3-Dimensional Morphological Analysis of Single Cells in the Zebrafish Left-right Organizer
    斑马鱼左右组织者中单细胞的嵌合标记和3D形态分析
    作者:Agnik Dasgupta, Andrew E. Jacob and Jeffrey D. Amack日期:11/20/2018,浏览量:203,Q&A: 0
    [Abstract] A transient epithelial structure called the left-right organizer (LRO) establishes left-right asymmetry in vertebrate embryos. Developmental defects that alter LRO formation result in left-right patterning errors that often lead to congenital heart malformations. However, little is known about mechanisms that regulate individual cell behaviors ...
    Microplastic Extraction from Marine Vertebrate Digestive Tracts, Regurgitates and Scats: A Protocol for Researchers from All Experience Levels
    海洋脊椎动物消化道,反流物和粪便中塑料微粒的提取:一个适用于所有经验水平研究者的实验方法
    作者:Amy L. Lusher and Gema Hernandez-Milian日期:11/20/2018,浏览量:589,Q&A: 0
    [Abstract] It is essential to provide a protocol for the separation and identification of microplastics in marine vertebrates (mammals, birds, turtles and fish) that is easy to follow and adaptable depending on research infrastructure. Digesting organic material is an effective way to analyze samples for microplastics. Presented here is an optimized protocol ...
    Zebrafish Embryo Xenograft and Metastasis Assay
    斑马鱼胚胎植入和转移
    作者:Ilkka Paatero, Sanni Alve, Silvia Gramolelli, Johanna Ivaska and Päivi M. Ojala日期:09/20/2018,浏览量:659,Q&A: 0
    [Abstract] Xenograft models, and in particular the mouse xenograft model, where human cancer cells are transplanted into immunocompromised mice, have been used extensively in cancer studies. Although these models have contributed enormously to our understanding of cancer biology, the zebrafish xenograft model offers several advantages over the mouse model. ...
    How to Catch a Smurf? – Ageing and Beyond…
    In vivo Assessment of Intestinal Permeability in Multiple Model Organisms

    如何捕捉蓝精灵? – 衰老及其他…
    多种模型生物体的肠道通透性体内评估
    作者:Raquel R. Martins, Andrew W. McCracken, Mirre J. P. Simons, Catarina M. Henriques and Michael Rera日期:02/05/2018,浏览量:2571,Q&A: 0
    [Abstract] The Smurf Assay (SA) was initially developed in the model organism Drosophila melanogaster where a dramatic increase of intestinal permeability has been shown to occur during aging (Rera et al., 2011). We have since validated the protocol in multiple other model organisms (Dambroise et al., 2016) and have utilized the ...
    Medaka-microinjection with an Upright Microscope
    在正置显微镜下进行青鳉显微注射
    作者:Yu Murakami and Masato Kinoshita日期:02/05/2018,浏览量:1506,Q&A: 0
    [Abstract] We described a simple method for microinjecting DNA/RNA/Protein solutions into medaka eggs under an upright microscope. Medaka is an excellent vertebrate model for reverse genetics, because of its daily spawning, short generation time, and egg transparency. These features enable us to efficiently perform functional genomic analyses of transgenic ...
    Fish Bile Clean-up for Subsequent Zymography and Mass Spectrometry Proteomic Analyses
    鱼胆汁清除后进行酶谱和质谱蛋白质组学分析
    作者:Rachel Ann Hauser-Davis日期:01/20/2018,浏览量:1828,Q&A: 0
    [Abstract] Biliary excretion offers a way to analyze various contaminants in aquatic organisms, and fish bile has been used as a biomarker for environmental contamination. The use of the fish bile proteome as a tool for monitoring the impact of environmental contaminants has been recently validated. However, scarce studies in this context are available, and ...
    Genotyping-free Selection of Double Allelic Gene Edited Medaka Using Two Different Fluorescent Proteins
    使用两种不同的荧光蛋白进行双等位基因编辑青鳉的无基因分型选择
    作者:Yu Murakami, Satoshi Ansai, Akari Yonemura and Masato Kinoshita日期:12/20/2017,浏览量:1845,Q&A: 0
    [Abstract] This protocol describes a simple genotyping using two different colors of fluorescent protein genes inserted at the target locus. This method makes it possible to determine the genotype of each individual simply by observing the fluorescence later than F1 generation.
    zPACT: Tissue Clearing and Immunohistochemistry on Juvenile Zebrafish Brain
    zPACT: 幼年斑马鱼脑的组织清除和免疫组化检测
    [Abstract] In studies of brain function, it is essential to understand the underlying neuro-architecture. Very young zebrafish larvae are widely used for neuroarchitecture studies, due to their size and natural transparency. However, this model system has several limitations, due to the immaturity, high rates of development and limited behavioral repertoire ...
    Bioluminescence Monitoring of Neuronal Activity in Freely Moving Zebrafish Larvae
    自由移动的斑马鱼幼鱼中神经元活性的生物发光监测
    作者:Steven Knafo, Andrew Prendergast, Olivier Thouvenin, Sophie Nunes Figueiredo and Claire Wyart日期:09/20/2017,浏览量:2874,Q&A: 0
    [Abstract] The proof of concept for bioluminescence monitoring of neural activity in zebrafish with the genetically encoded calcium indicator GFP-aequorin has been previously described (Naumann et al., 2010) but challenges remain. First, bioluminescence signals originating from a single muscle fiber can constitute a major pitfall. Second, ...