蠕虫

分类

    现刊
    Blinded Visual Scoring of Images Using the Freely-available Software Blinder
    使用免费软件Blinder对图像进行盲视觉评分
    作者:Steven D. Cothren, Joel N. Meyer and Jessica H. Hartman日期:12/05/2018,浏览量:75,Q&A: 0
    In nearly all subfields of biomedical sciences, there are phenotypes that are currently classified by expert visual scoring. In research applications, these classifications require the experimenter to be blinded to the treatment group in order to ...
    Blinded Visual Scoring of Images Using the Freely-available Software Blinder
    使用免费软件Blinder对图像进行盲视觉评分
    作者:Steven D. Cothren, Joel N. Meyer and Jessica H. Hartman日期:12/05/2018,浏览量:75,Q&A: 0
    [Abstract] In nearly all subfields of biomedical sciences, there are phenotypes that are currently classified by expert visual scoring. In research applications, these classifications require the experimenter to be blinded to the treatment group in order to avoid unintentional bias in scoring. Currently, many labs either use laborious and tedious methods to ...
    Staining the Germline in Live Caenorhabditis elegans: Overcoming Challenges by Applying a Fluorescent-dye Feeding Strategy
    利用荧光染料喂食的方法活体染色标记秀丽隐杆线虫生殖腺
    作者:K. Adam Bohnert日期:11/05/2018,浏览量:299,Q&A: 0
    [Abstract] C. elegans provides a tractable model organism for studying germline cell biology. Microscopy experiments are relatively facile, as this worm is transparent and germline development can be observed in real-time using DIC microscopy and/or fluorescent transgenes. Despite these many tools, robust staining techniques for imaging germ cells ...
    Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans
    秀丽隐杆线虫中细胞核/细胞质蛋白的分离
    作者:Alejandro Mata-Cabana, Olga Sin, Renée I. Seinstra and Ellen A. A. Nollen日期:10/20/2018,浏览量:630,Q&A: 0
    [Abstract] C. elegans is widely used to investigate biological processes related to health and disease. To study protein localization, fluorescently-tagged proteins can be used in vivo or immunohistochemistry can be performed in whole worms. Here, we describe a technique to localize a protein of interest at a subcellular level in C. elegans ...
    Filter Retardation Assay for Detecting and Quantifying Polyglutamine Aggregates Using Caenorhabditis elegans Lysates
    使用秀丽隐杆线虫裂解液检测和定量聚谷氨酰胺聚集体的滤器延迟试验
    作者:Olga Sin, Alejandro Mata-Cabana, Renée I. Seinstra and Ellen A. A. Nollen日期:10/05/2018,浏览量:459,Q&A: 0
    [Abstract] Protein aggregation is a hallmark of several neurodegenerative diseases and is associated with impaired protein homeostasis. This imbalance is caused by the loss of the protein’s native conformation, which ultimately results in its aggregation or abnormal localization within the cell. Using a C. elegans model of polyglutamine diseases, we ...
    Maintenance of Schmidtea mediterranea in the Laboratory
    实验室真涡虫的保存
    作者:Nídia de Sousa and Teresa Adell日期:10/05/2018,浏览量:268,Q&A: 0
    [Abstract] In the last years, planarians have emerged as a unique model animal for studying regeneration and stem cells biology. Although their remarkable regenerative abilities are known for a long time, only recently the molecular tools to understand the biology of planarian stem cells and the fundamentals of their regenerative process have been ...
    Detection of Cell Death in Planarians
    真涡虫细胞死亡的检测
    作者:Nídia de Sousa and Teresa Adell日期:10/05/2018,浏览量:475,Q&A: 0
    [Abstract] Planarians are freshwater flatworms, well known for their ability to regenerate a complete organism from any piece of their body. Furthermore, planarians are constantly growing and degrowing throughout their lives, maintaining a functional and proportioned body. These properties rely on the presence of a population of adult stem cells and on the ...
    6-hydroxydopamine (6-OHDA) Oxidative Stress Assay for Observing Dopaminergic Neuron Loss in Caenorhabditis elegans
    6-羟多巴胺(6-OHDA)氧化应激实验分析观察秀丽隐杆线虫多巴胺能神经元的损失
    作者:Sarah-Lena Offenburger and Anton Gartner日期:09/20/2018,浏览量:395,Q&A: 0
    [Abstract] The nematode Caenorhabditis elegans is a powerful genetic model that can be used to investigate neuronal death. Research using C. elegans has been crucial to characterize cell death programmes that are conserved in mammals. Many neuronal signaling components, such as those mediating dopaminergic neurotransmission, are preserved ...
    A Behavioral Assay to Examine the Effects of Kavalactones on Caenorhabditis elegans Neuromuscular Excitability
    行为分析检测卡瓦内酯对于秀丽隐杆线虫神经肌肉兴奋性的影响
    作者:Bwarenaba B. Kautu, Jessie Chappel, Kellie Steele, Juliana Phillips and M. Shawn Mengarelli日期:09/20/2018,浏览量:437,Q&A: 0
    [Abstract] Kavalactones are a class of lactone compounds found in Kava, a traditional beverage from the South Pacific Islands that is derived from the root of Piper methysticum. When consumed, these compounds produce sedative and anxiolytic effects, suggesting their potent actions on the nervous system. Here, we provide a protocol to examine the ...
    Pentylenetetrazole (PTZ)-induced Convulsion Assay to Determine GABAergic Defects in Caenorhabditis elegans
    用于确定秀丽隐杆线虫γ-氨基丁酸能缺陷的戊四氮唑诱导的惊厥测定试验
    作者:Shruti Thapliyal and Kavita Babu日期:09/05/2018,浏览量:432,Q&A: 0
    [Abstract] Pentylenetetrazole (PTZ) is a GABAA receptor antagonist and is used to monitor presynaptic defects in the release of the inhibitory neurotransmitter GABA. PTZ is a competitive inhibitor of GABA, and prevents binding of GABA on the GABAA receptors present on the surface of muscle. In the absence of GABA binding, the excitatory ...
    Assessment of Caenorhabditis elegans Competitive Fitness in the Presence of a Bacterial Parasite
    寄生菌存在情况下秀丽隐杆线虫的竞争适应性测定
    作者:McKenna. J. Penley and Levi T. Morran日期:08/20/2018,浏览量:579,Q&A: 0
    [Abstract] Accurate measurements of an organism’s fitness are crucial for measuring evolutionary change. Methods of fitness measurement are most accurate when incorporating an individual’s survival and fecundity, as well as accounting for any ecological interactions or environmental effects experienced by the organism. Here, we describe a protocol for ...