搜索

植物

分类

    现刊
    Electron Tomography to Study the Three-dimensional Structure of Plasmodesmata in Plant Tissues–from High Pressure Freezing Preparation to Ultrathin Section Collection
    采用电子断层摄影术研究植物组织中的胞间连丝的三维结构 — 从高压冷冻制备到超薄切片采集
    作者:William J. Nicolas, Emmanuelle Bayer and Lysiane Brocard日期:01/05/2018,浏览量:186,Q&A: 0
    Plasmodesmata (PD) are nanometric (~20 nm wide) membrane lined pores encased in the cell walls of the adjacent plant cells. They allow the cells to exchange all types of molecules ranging from nutrients like sugar, hormones, to RNAs and various ...
    In vitro RNA-dependent RNA Polymerase Assay Using Arabidopsis RDR6
    拟南芥RDR6的体外RNA依赖性RNA聚合酶活性测定
    作者:Kyungmin Baeg, Yukihide Tomari and Hiro-oki Iwakawa日期:01/05/2018,浏览量:117,Q&A: 0
    RNA-dependent RNA polymerases (RdRPs) in eukaryotes convert single-stranded RNAs into double-stranded RNAs, thereby amplifying small interfering RNAs that play crucial roles in the regulation of development, maintenance of genome integrity and ...
    Micro-computed Tomography to Visualize Vascular Networks in Maize Stems
    玉米茎杆中维管网络的微型计算机断层摄影术观察
    作者:Akiteru Maeno and Katsutoshi Tsuda日期:01/05/2018,浏览量:175,Q&A: 0
    Plant vascular systems in the stem connect roots with aerial organs to move solutes containing minerals, nutrients as well as signaling molecules, and therefore, they play pivotal roles in plant growth and development. However, stem vascular ...
    Chromatin Affinity Purification (ChAP) from Arabidopsis thaliana Rosette Leaves Using in vivo Biotinylation System
    使用体内生物素化系统进行拟南芥莲座叶染色质亲和纯化(ChAP)
    作者:Weronika Sura and Piotr A. Ziolkowski日期:01/05/2018,浏览量:117,Q&A: 0
    Chromatin Affinity Purification (ChAP) is widely used to study chromatin architecture and protein complexes interacting with DNA. Here we present an efficient method for ChAP from Arabidopsis thaliana rosette leaves, in which in vivo ...
    Real-time Analysis of Auxin Response, Cell Wall pH and Elongation in Arabidopsis thaliana Hypocotyls
    拟南芥下胚轴植物生长素响应、细胞壁pH和伸长的实时分析
    作者:Lanxin Li, S. F. Gabriel Krens, Matyáš Fendrych and Jiří Friml日期:01/05/2018,浏览量:268,Q&A: 0
    The rapid auxin-triggered growth of the Arabidopsis hypocotyls involves the nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification of the apoplast and cell walls (Fendrych et al., 2016). Here, we describe in detail ...
    Fatty Acid Content and Composition of Triacylglycerols of Chlorella kessleri
    凯氏小球藻中脂肪酸含量及三酰甘油组成
    作者:Motohide Aoki and Norihiro Sato日期:01/05/2018,浏览量:110,Q&A: 0
    Triacylglycerols (TAGs) are esters formed from one glycerol and three fatty acids. TAGs are induced to accumulate in algal cells under environmental stress conditions including nutrient-limitation, hyperosmosis, and low temperature, for the storage ...
    Rolling Circle Amplification to Screen Yam Germplasm for Badnavirus Infections and to Amplify and Characterise Novel Badnavirus Genomes
    滚换扩增筛选山药Badnavirus感染种质并扩增鉴定新型Badnavirus基因组
    作者:Moritz Bömer, Aliyu A. Turaki, Ajith I. Rathnayake, Gonçalo Silva, P. Lava Kumar and Susan E. Seal日期:01/05/2018,浏览量:115,Q&A: 0
    Since the first discovery of badnaviruses (family Caulimoviridae, genus Badnavirus) in yam (Dioscorea spp.) germplasm in the 1970s (Harrison and Roberts, 1973), several hundred partial badnavirus reverse transcriptase ...
    Electron Tomography to Study the Three-dimensional Structure of Plasmodesmata in Plant Tissues–from High Pressure Freezing Preparation to Ultrathin Section Collection
    采用电子断层摄影术研究植物组织中的胞间连丝的三维结构 — 从高压冷冻制备到超薄切片采集
    作者:William J. Nicolas, Emmanuelle Bayer and Lysiane Brocard日期:01/05/2018,浏览量:186,Q&A: 0
    [Abstract] Plasmodesmata (PD) are nanometric (~20 nm wide) membrane lined pores encased in the cell walls of the adjacent plant cells. They allow the cells to exchange all types of molecules ranging from nutrients like sugar, hormones, to RNAs and various proteins. Unfortunately, they are also hijacked by phyto-viruses, enabling them to spread from ...
    In vitro RNA-dependent RNA Polymerase Assay Using Arabidopsis RDR6
    拟南芥RDR6的体外RNA依赖性RNA聚合酶活性测定
    作者:Kyungmin Baeg, Yukihide Tomari and Hiro-oki Iwakawa日期:01/05/2018,浏览量:117,Q&A: 0
    [Abstract] RNA-dependent RNA polymerases (RdRPs) in eukaryotes convert single-stranded RNAs into double-stranded RNAs, thereby amplifying small interfering RNAs that play crucial roles in the regulation of development, maintenance of genome integrity and antiviral immunity. Here, we describe a method of in vitro RdRP assay using recombinant ...
    Micro-computed Tomography to Visualize Vascular Networks in Maize Stems
    玉米茎杆中维管网络的微型计算机断层摄影术观察
    作者:Akiteru Maeno and Katsutoshi Tsuda日期:01/05/2018,浏览量:175,Q&A: 0
    [Abstract] Plant vascular systems in the stem connect roots with aerial organs to move solutes containing minerals, nutrients as well as signaling molecules, and therefore, they play pivotal roles in plant growth and development. However, stem vascular systems, especially in crop species, have been poorly described since they are deeply embedded in the ...
    Chromatin Affinity Purification (ChAP) from Arabidopsis thaliana Rosette Leaves Using in vivo Biotinylation System
    使用体内生物素化系统进行拟南芥莲座叶染色质亲和纯化(ChAP)
    作者:Weronika Sura and Piotr A. Ziolkowski日期:01/05/2018,浏览量:117,Q&A: 0
    [Abstract] Chromatin Affinity Purification (ChAP) is widely used to study chromatin architecture and protein complexes interacting with DNA. Here we present an efficient method for ChAP from Arabidopsis thaliana rosette leaves, in which in vivo biotinylation system is used. The chromatin is digested by Micrococcal Nuclease (MNase), hence ...
    Real-time Analysis of Auxin Response, Cell Wall pH and Elongation in Arabidopsis thaliana Hypocotyls
    拟南芥下胚轴植物生长素响应、细胞壁pH和伸长的实时分析
    作者:Lanxin Li, S. F. Gabriel Krens, Matyáš Fendrych and Jiří Friml日期:01/05/2018,浏览量:268,Q&A: 0
    [Abstract] The rapid auxin-triggered growth of the Arabidopsis hypocotyls involves the nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification of the apoplast and cell walls (Fendrych et al., 2016). Here, we describe in detail the method for analysis of the elongation and the TIR1/AFB-Aux/IAA-dependent auxin response in ...
    Fatty Acid Content and Composition of Triacylglycerols of Chlorella kessleri
    凯氏小球藻中脂肪酸含量及三酰甘油组成
    作者:Motohide Aoki and Norihiro Sato日期:01/05/2018,浏览量:110,Q&A: 0
    [Abstract] Triacylglycerols (TAGs) are esters formed from one glycerol and three fatty acids. TAGs are induced to accumulate in algal cells under environmental stress conditions including nutrient-limitation, hyperosmosis, and low temperature, for the storage of metabolic energy and carbon, and also for the consumption of excess energy (e.g., Hirai ...
    Rolling Circle Amplification to Screen Yam Germplasm for Badnavirus Infections and to Amplify and Characterise Novel Badnavirus Genomes
    滚换扩增筛选山药Badnavirus感染种质并扩增鉴定新型Badnavirus基因组
    作者:Moritz Bömer, Aliyu A. Turaki, Ajith I. Rathnayake, Gonçalo Silva, P. Lava Kumar and Susan E. Seal日期:01/05/2018,浏览量:115,Q&A: 0
    [Abstract] Since the first discovery of badnaviruses (family Caulimoviridae, genus Badnavirus) in yam (Dioscorea spp.) germplasm in the 1970s (Harrison and Roberts, 1973), several hundred partial badnavirus reverse transcriptase (RT)-ribonuclease H (RNaseH) sequences have been characterised (Kenyon et al., 2008; Bousalem ...
    Infection of Soybean Plants with the Insect Bacterial Symbiont Burkholderia gladioli and Evaluation of Plant Fitness
    用昆虫细菌共生体唐菖蒲伯克霍尔德菌感染大豆植株及植物适应性评价
    作者:Paul Gaube, Martin Kaltenpoth and Laura V. Flórez日期:12/20/2017,浏览量:399,Q&A: 0
    [Abstract] To investigate the establishment and consequences of host-microbe interactions, it is important to develop controlled infection assays suitable for each system, as well as appropriate methods to evaluate successful infection and its associated effects. Here, we describe a procedure for bacterial inoculation of soybean plants, followed by the ...
    Biomechanical Characterization of Onion Epidermal Cell Walls
    洋葱表皮细胞壁的生物力学表征
    作者:Daniel M. Durachko, Yong Bum Park, Tian Zhang and Daniel J. Cosgrove日期:12/20/2017,浏览量:278,Q&A: 0
    [Abstract] Here we describe two experimental protocols to measure the biomechanical properties of primary (growing) plant cell walls, with a focus on analyzing cell wall epidermal strips of onion scales. The first protocol measures cell wall creep (time-dependent irreversible extension) under constant force. Such creep is often mediated by the wall-loosening ...
    Preparation of Onion Epidermal Cell Walls for Imaging by Atomic Force Microscopy (AFM)
    制备洋葱表皮细胞壁用于原子力显微镜(AFM)成像
    作者:Tian Zhang and Daniel J. Cosgrove日期:12/20/2017,浏览量:421,Q&A: 0
    [Abstract] The growing plant cell wall is comprised of long, thin cellulose microfibrils embedded in a hydrated matrix of polysaccharides and glycoproteins. These components are typically constructed in layers (lamellae) on the inner surface of the cell wall, i.e., between the existing wall and the plasma membrane. The organization of these ...