植物

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    现刊
    An Adjustable Protocol to Analyze Chemical Profiles of Non-sterile Rhizosphere Soil
    一种可调整的用于分析研究非无菌根际土壤化学特性的方法
    作者:Alex Williams, Jurriaan Ton and Pierre Pétriacq日期:05/20/2019,浏览量:282,Q&A: 0
    The analysis of chemical diversity in non-sterile rhizosphere soil has been a pressing methodological challenge for years. Rhizosphere-enriched chemicals (i.e., rhizochemicals) include root exudation chemicals, (microbial) breakdown ...
    An Improved Bioassay to Study Arabidopsis Induced Systemic Resistance (ISR) Against Bacterial Pathogens and Insect Pests
    一种的可用于检测拟南芥中细菌性病原体和害虫诱导的系统抗性的改进生物学方法
    作者:Nicolás M. Cecchini, Yi Song, Suruchi Roychoudhry, Jean T. Greenberg and Cara H. Haney日期:05/20/2019,浏览量:421,Q&A: 0
    The plant immune system is essential for plants to perceive and defend against bacterial, fungal and insect pests and pathogens. Induced systemic resistance (ISR) is a systemic immune response that occurs upon root colonization by beneficial ...
    An Adjustable Protocol to Analyze Chemical Profiles of Non-sterile Rhizosphere Soil
    一种可调整的用于分析研究非无菌根际土壤化学特性的方法
    作者:Alex Williams, Jurriaan Ton and Pierre Pétriacq日期:05/20/2019,浏览量:282,Q&A: 0
    [Abstract] The analysis of chemical diversity in non-sterile rhizosphere soil has been a pressing methodological challenge for years. Rhizosphere-enriched chemicals (i.e., rhizochemicals) include root exudation chemicals, (microbial) breakdown products thereof, and de novo produced metabolites by rhizosphere-inhabiting microbes, all of ...
    An Improved Bioassay to Study Arabidopsis Induced Systemic Resistance (ISR) Against Bacterial Pathogens and Insect Pests
    一种的可用于检测拟南芥中细菌性病原体和害虫诱导的系统抗性的改进生物学方法
    作者:Nicolás M. Cecchini, Yi Song, Suruchi Roychoudhry, Jean T. Greenberg and Cara H. Haney日期:05/20/2019,浏览量:421,Q&A: 0
    [Abstract] The plant immune system is essential for plants to perceive and defend against bacterial, fungal and insect pests and pathogens. Induced systemic resistance (ISR) is a systemic immune response that occurs upon root colonization by beneficial microbes. A well-studied model for ISR is the association of specific beneficial strains of Pseudomonas ...
    Determination of Root Exudate Concentration in the Rhizosphere Using 13C Labeling
    13C标记测定根际根系分泌物浓度
    作者:Laurent Simon and Feth el Zahar Haichar日期:05/05/2019,浏览量:259,Q&A: 0
    [Abstract] One of the most remarkable metabolic features of plant roots is their ability to secrete a wide range of compounds into the rhizosphere, defined as the volume of soil around living roots. Around 5%-21% of total photosynthetically fixed carbon is transferred into the rhizosphere through root exudates. Until recently, studies on the quantity and ...
    Tracking Root Interactions System (TRIS) Experiment and Quality Control
    根相互作用系统的示踪实验和质量控制
    作者:Hassan Massalha, Elisa Korenblum, Orr H. Shapiro and Asaph Aharoni日期:04/20/2019,浏览量:650,Q&A: 0
    [Abstract] Soil organisms are diverse taxonomically and functionally. This ecosystem experiences highly complex networks of interactions, but may also present functionally independent entities. Plant roots, a metabolically active hotspot in the soil, take an essential part in shaping the rhizosphere. Tracking the dynamics of root-microbe interactions at high ...
    Quantification of the Humidity Effect on HR by Ion Leakage Assay
    离子渗漏实验定量分析湿度对超敏反应的影响
    作者:Musoki Mwimba and Xinnian Dong日期:04/05/2019,浏览量:402,Q&A: 0
    [Abstract] We describe a protocol to measure the contribution of humidity on cell death during the effector-triggered immunity (ETI), the plant immune response triggered by the recognition of pathogen effectors by plant resistance genes. This protocol quantifies tissue cell death by measuring ion leakage due to loss of membrane integrity during the ...
    Visualization of Plant Cell Wall Epitopes Using Immunogold Labeling for Electron Microscopy
    利用电子显微镜免疫金标记法进行植物细胞壁表位的可视化观察
    作者:Mateusz Majda日期:04/05/2019,浏览量:585,Q&A: 0
    [Abstract] Plant cell walls consist of different polysaccharides and structural proteins, which form a rigid layer located outside of the plasma membrane. The wall is also a very dynamic cell composite, which is characterized by complex polysaccharide interactions and various modifications during cell development. The visualization of cell wall components in ...
    Extraction and Purification of Laccases from Rice Stems
    水稻茎秆酶的提取与纯化
    作者:Chenna Swetha and P. V. Shivaprasad日期:04/05/2019,浏览量:391,Q&A: 0
    [Abstract] Laccases are found in cell walls of plants in very low amounts. This protocol provides an efficient method to purify laccases from rice stems. The method involves three steps: 1) Isolation of total protein from rice stems using buffers with high salt concentration to extract protein from cell walls; 2) Purification of laccases using concanavalin-A ...
    Quantitative Plasmodesmata Permeability Assay for Pavement Cells of Arabidopsis Leaves
    拟南芥叶片扁平细胞中胞间连丝渗透性定量测定实验
    作者:Min Diao, Qiannan Wang and Shanjin Huang日期:04/05/2019,浏览量:533,Q&A: 0
    [Abstract] Plasmodesmata (PD) are intercellular channels between walled plant cells that enable the transportation of materials between adjacent cells, which are important for plant growth and development. The permeability of PD must be tightly regulated. Assays to determine the permeability of PD are crucial for related studies on the regulation of PD ...
    Multiple Modification of Chromosomal Loci Using URA5.3 Selection Marker in the Unicellular Red Alga Cyanidioschyzon merolae
    在单细胞红藻 Cyanidioschyzon merolae中利用URA5.3选择标记对染色体基因进行多重修饰
    作者:Tokiaki Takemura, Sousuke Imamura, Yuki Kobayashi and Kan Tanaka日期:04/05/2019,浏览量:373,Q&A: 0
    [Abstract] The unicellular red alga Cyanidioschyzon merolae has been used as a eukaryotic photosynthetic model for various basic and applied studies. Although the nuclear genome of C. merolae can be modified by homologous recombination with exogenously introduced DNA, it has been difficult to modify multiple chromosome loci within the same ...
    Peptide Feeding and Mechanical Wounding for Tomato Seedlings
    番茄幼苗的肽饲喂和机械伤害
    作者:Lei Wang, Yan Wang and Georg Felix日期:03/20/2019,浏览量:803,Q&A: 0
    [Abstract] Plants need to respond appropriately to wounding and herbivorous insects. Peptide signals have been implicated in local and systemic induction of appropriate plant defense responses. To study these peptide signals and their perception in host plants, it is important to have reproducible bioassays. Several assays, such as treatment of peptide ...