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病毒

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    现刊
    Targeted Genome Editing of Virulent Phages Using CRISPR-Cas9
    使用CRISPR-Cas9对烈性噬菌体进行靶向基因组编辑
    作者:Marie-Laurence Lemay, Ariane C. Renaud, Geneviève M. Rousseau and Sylvain Moineau日期:01/05/2018,浏览量:219,Q&A: 0
    This protocol describes a straightforward method to generate specific mutations in the genome of strictly lytic phages. Briefly, a targeting CRISPR-Cas9 system and a repair template suited for homologous recombination are provided inside a bacterial ...
    Rolling Circle Amplification to Screen Yam Germplasm for Badnavirus Infections and to Amplify and Characterise Novel Badnavirus Genomes
    滚换扩增筛选山药Badnavirus感染种质并扩增鉴定新型Badnavirus基因组
    作者:Moritz Bömer, Aliyu A. Turaki, Ajith I. Rathnayake, Gonçalo Silva, P. Lava Kumar and Susan E. Seal日期:01/05/2018,浏览量:115,Q&A: 0
    Since the first discovery of badnaviruses (family Caulimoviridae, genus Badnavirus) in yam (Dioscorea spp.) germplasm in the 1970s (Harrison and Roberts, 1973), several hundred partial badnavirus reverse transcriptase ...
    Targeted Genome Editing of Virulent Phages Using CRISPR-Cas9
    使用CRISPR-Cas9对烈性噬菌体进行靶向基因组编辑
    作者:Marie-Laurence Lemay, Ariane C. Renaud, Geneviève M. Rousseau and Sylvain Moineau日期:01/05/2018,浏览量:219,Q&A: 0
    [Abstract] This protocol describes a straightforward method to generate specific mutations in the genome of strictly lytic phages. Briefly, a targeting CRISPR-Cas9 system and a repair template suited for homologous recombination are provided inside a bacterial host, here the Gram-positive model Lactococcus lactis MG1363. The CRISPR-Cas9 system is ...
    Rolling Circle Amplification to Screen Yam Germplasm for Badnavirus Infections and to Amplify and Characterise Novel Badnavirus Genomes
    滚换扩增筛选山药Badnavirus感染种质并扩增鉴定新型Badnavirus基因组
    作者:Moritz Bömer, Aliyu A. Turaki, Ajith I. Rathnayake, Gonçalo Silva, P. Lava Kumar and Susan E. Seal日期:01/05/2018,浏览量:115,Q&A: 0
    [Abstract] Since the first discovery of badnaviruses (family Caulimoviridae, genus Badnavirus) in yam (Dioscorea spp.) germplasm in the 1970s (Harrison and Roberts, 1973), several hundred partial badnavirus reverse transcriptase (RT)-ribonuclease H (RNaseH) sequences have been characterised (Kenyon et al., 2008; Bousalem ...
    Rice Black-streaked Dwarf Virus Preparation and Infection on Rice
    水稻黑条矮缩病病毒的制备及其对水稻的感染
    作者:Zongtao Sun, Hehong Zhang, Kaili Xie, Xiaoxiang Tan, Hengmu Zhang and Jianping Chen日期:12/20/2017,浏览量:317,Q&A: 0
    [Abstract] Rice black-streaked dwarf virus (RBSDV), a member of genus Fijivirus in the family Reoviridae, infects rice, maize, barley and wheat, and can seriously affect crop yields. RBSDV is transmitted by the small brown planthopper (Laodelphax striatellus, SBPH) in a persistent manner. RBSDV has 10 linear dsRNA genomic segments, ...
    Lentiviral Knockdown of Transcription Factor STAT1 in Peromyscus leucopus to Assess Its Role in the Restriction of Tick-borne Flaviviruses
    慢病毒敲减白足鼠中转录因子STAT1以评价其在抑制蜱传黄病毒中的作用
    作者:Adaeze O. Izuogu and R. Travis Taylor日期:12/05/2017,浏览量:376,Q&A: 0
    [Abstract] Cellular infection with tick-borne flaviviruses (TBFVs) results in activation of the interferon (IFN) signaling pathway and subsequent upregulation of numerous genes termed IFN stimulated genes (ISGs) (Schoggins et al., 2011). Many ISGs function to prevent virus pathogenesis by acting in a broad or specific manner through protein-protein ...
    Isolation, BODIPY Labeling and Uptake of Exosomes in Hepatic Stellate Cells
    肝星状细胞中外泌体的分离、BODIPY标记和摄取
    作者:Shubham Shrivastava日期:12/05/2017,浏览量:425,Q&A: 0
    [Abstract] Exosomes have emerged as an important mediator of intercellular communication. They are present in extracellular milieu and therefore, easily accessible by neighboring or distant cells. They carry mRNA, microRNAs and proteins within their vesicles and once internalized by recipient cells; they can modulate multiple signaling pathways with ...
    Organotypic Brain Cultures: A Framework for Studying CNS Infection by Neurotropic Viruses and Screening Antiviral Drugs
    器官型脑培养:研究嗜神经病毒对中枢神经系统的感染和筛选抗病毒药物的框架
    [Abstract] According to the World Health Organization (WHO), at least 50% of emerging viruses endowed with pathogenicity in humans can infect the Central Nervous System (CNS) with induction of encephalitis and other neurologic diseases (Taylor et al., 2001; Olival and Daszak, 2005). While neurological diseases are progressively documented, the ...
    Isolation of Rice Stripe Virus Preparation from Viruliferous Small Brown Planthoppers and Mechanic Inoculation on Rice
    从带病毒的灰飞虱中分离水稻条纹病毒制备物并机械接种于水稻
    作者:Wan Zhao, Le Kang and Feng Cui日期:11/05/2017,浏览量:462,Q&A: 0
    [Abstract] Tenuiviruses can infect the plants of the family Poaceae, and cause serious loss of crops, particularly rice and maize, in South-Eastern Asian countries. Tenuiviruses usually depend on insect vectors for their transmission and cannot be transmitted between plants through wounds or abrasions. Rice stripe virus (RSV), a typical member of ...
    A Bioreactor Method to Generate High-titer, Genetically Stable, Clinical-isolate Human Cytomegalovirus
    能产生高滴度、遗传稳定、临床分离人巨细胞病毒的一种生物反应器方法
    [Abstract] Human cytomegalovirus (HCMV) infection is a major cause of morbidity and mortality in transplant patients and a leading cause of congenital birth defects (Saint Louis, 2016). Vaccination and therapeutic studies often require scalable cell culture production of wild type virus, represented by clinical isolates. Obtaining sufficient stocks of ...
    Multicolor Stimulated Emission Depletion (STED) Microscopy to Generate High-resolution Images of Respiratory Syncytial Virus Particles and Infected Cells
    利用多色受激发射损耗(STED)显微镜获取呼​​吸道合胞体病毒颗粒和感染细胞的高分辨率图像
    作者:Masfique Mehedi, Margery Smelkinson, Juraj Kabat, Sundar Ganesan, Peter L. Collins and Ursula J. Buchholz日期:09/05/2017,浏览量:1010,Q&A: 0
    [Abstract] Human respiratory syncytial virus (RSV) infection in human lung epithelial A549 cells induces filopodia, cellular protrusions consisting of F-actin, that extend to neighboring uninfected cells (Mehedi et al., 2016). High-resolution imaging via stimulated emission depletion (STED) microscopy revealed filamentous RSV particles along these ...
    An Optimized Method for the Production Using PEI, Titration and Neutralization of SARS-CoV Spike Luciferase Pseudotypes
    利用PEI优化生产,滴定和中和SARS-CoV spike萤光素酶假病毒的方法
    作者:George Carnell, Keith Grehan, Francesca Ferrara, Eleonora Molesti and Nigel Temperton日期:08/20/2017,浏览量:1450,Q&A: 0
    [Abstract] The protocol outlined represents a cost-effective, rapid and reliable method for the generation of high-titre viral pseudotype particles with the wild-type SARS-CoV spike protein on a lentiviral vector core using the widely available transfection reagent PEI. This protocol is optimized for transfection in 6-well plates; however it can be readily ...