病毒

分类

    现刊
    In vitro Membrane Interaction and Liposome Fusion Assays Using Recombinant Hepatitis C Virus Envelope Protein E2
    利用重组丙型肝炎病毒包膜蛋白E2体外检测膜互作和脂质体融合
    作者:Belén Yélamos, Mar Rodríguez-Rodríguez, Francisco Gavilanes and Julián Gómez-Gutiérrez日期:12/05/2018,浏览量:39,Q&A: 0
    In order to study the mechanism underlying the Hepatitis C Virus (HCV) fusion process we have performed assays using phospholipid liposomes and a truncated form of E2 protein, E2661 (amino acids 384-661 of the HCV polyprotein) lacking the ...
    In vitro Membrane Interaction and Liposome Fusion Assays Using Recombinant Hepatitis C Virus Envelope Protein E2
    利用重组丙型肝炎病毒包膜蛋白E2体外检测膜互作和脂质体融合
    作者:Belén Yélamos, Mar Rodríguez-Rodríguez, Francisco Gavilanes and Julián Gómez-Gutiérrez日期:12/05/2018,浏览量:39,Q&A: 0
    [Abstract] In order to study the mechanism underlying the Hepatitis C Virus (HCV) fusion process we have performed assays using phospholipid liposomes and a truncated form of E2 protein, E2661 (amino acids 384-661 of the HCV polyprotein) lacking the transmembrane region. E2661 has been previously generated by using the baculovirus ...
    Quantification of Infectious Sendai Virus Using Plaque Assay
    利用空斑实验量化分析传染性仙台病毒
    作者:Narihito Tatsumoto, Takamasa Miyauchi, Moshe Arditi and Michifumi Yamashita日期:11/05/2018,浏览量:225,Q&A: 0
    [Abstract] Sendai virus (SeV) is an enveloped, single-stranded RNA virus of the family Paramyxoviridae. SeV is a useful tool to study its infectious pathomechanism in immunology and the pathomechanism of a murine model of IgA nephropathy. Virus quantification is essential not only to determine the original viral titers for an appropriate ...
    Rice Ragged Stunt Virus Propagation and Infection on Rice Plants
    稻株中水稻齿叶矮缩病毒的传播和感染
    作者:Chao Zhang, Chaonan Shi, Dong Chen and Jianguo Wu日期:10/20/2018,浏览量:262,Q&A: 0
    [Abstract] Virus inoculation is a basic experimental procedure to evaluate the resistance of a rice variety or a transgenic material upon virus infection. We recently demonstrated that Rice Ragged Stunt Virus (RRSV), an oryzavirus that is transmitted by brown planthopper (BPH), can suppress jasmonic acid-mediated antiviral defense through ...
    Detection and Differentiation of Multiple Viral RNAs Using Branched DNA FISH Coupled to Confocal Microscopy and Flow Cytometry
    分支DNA FISH技术联合共聚焦显微镜或流式细胞术检测和鉴别多样的病毒RNA
    作者:Nicholas van Buuren and Karla Kirkegaard日期:10/20/2018,浏览量:428,Q&A: 0
    [Abstract] Due to the exceptionally high mutation rates of RNA-dependent RNA polymerases, infectious RNA viruses generate extensive sequence diversity, leading to some of the lowest barriers to the development of antiviral drug resistance in the microbial world. We have previously discovered that higher barriers to the development of drug resistance can be ...
    HIVGKO: A Tool to Assess HIV-1 Latency Reversal Agents in Human Primary CD4+ T Cells
    gHIVGKO:一种用于检测人原代CD4+T细胞中艾滋病潜伏期逆转剂的工具
    作者:Emilie Battivelli and Eric Verdin日期:10/20/2018,浏览量:326,Q&A: 0
    [Abstract] While able to suppress HIV replication in HIV infected individuals, combination antiretroviral therapy (ART) fails to eliminate viral latent reservoir, which consists in integrated transcriptional silenced HIV provirus. So far, identification of latently-infected cells has relied on activating cells to induce expression of HIV proteins which can ...
    H1N1 Virus Production and Infection
    H1N1病毒的制备和感染
    作者:Binbin Zhao, Jiaoyu Shan, Rui Xiong, Ke Xu and Bin Li日期:10/20/2018,浏览量:251,Q&A: 0
    [Abstract] Influenza A virus is a member of orthomyxoviridae family causing wide-spread infections in human respiratory tract. Mouse infection model is widely used in antiviral research and pathogenesis study against influenza A virus. Here, we report a protocol in infected mice with different virus doses and strains to explore how an inhibitor of ...
    Expression and Ni-NTA-Agarose Purification of Recombinant Hepatitis C Virus E2 Ectodomain Produced in a Baculovirus Expression System
    在杆状病毒表达系统中重组丙型肝炎病毒E2胞外结构域的表达和Ni-NTA-琼脂糖纯化
    作者:Julián Gómez-Gutiérrez, Mar Rodríguez-Rodríguez, Francisco Gavilanes and Belén Yélamos日期:10/05/2018,浏览量:281,Q&A: 0
    [Abstract] In this protocol, we describe the production and purification of the ectodomain of the E2661 envelope protein (amino acids 384-661) of the Hepatitis C virus, which plays a fundamental role in the entry of the virus into the host cell. This protein has been expressed in both prokaryotic and eukaryotic systems but in small quantities or ...
    Sendai Virus Propagation Using Chicken Eggs
    利用鸡蛋进行仙台病毒的繁殖
    作者:Narihito Tatsumoto, Moshe Arditi and Michifumi Yamashita日期:09/20/2018,浏览量:439,Q&A: 0
    [Abstract] Sendai virus is a member of the family Paramyxoviridae, and an enveloped virus with a negative-stranded RNA genome. Sendai virus is not pathogenic to humans, but for mice and can cause pneumonia in mice. Easy and efficient techniques for propagating Sendai virus are required for studying virus replication, virus-induced innate- and ...
    Retroviral Capsid Core Stability Assay
    逆转录病毒核衣壳稳定性检测
    作者:Tyler Milston Renner, Kasandra Bélanger and Marc-André Langlois日期:09/20/2018,浏览量:573,Q&A: 0
    [Abstract] Structural stability of the capsid core is a critical parameter for the productive infection of a cell by a retrovirus. Compromised stability can lead to premature core disassembly, exposure of replication intermediates to cytosolic nucleic acid sensors that can trigger innate antiviral responses, and failure to integrate the proviral genome into ...
    Selective Isolation of Retroviruses from Extracellular Vesicles by Intact Virion Immunoprecipitation
    通过完整病毒免疫沉淀技术从细胞外囊泡选择性分离逆转录病毒
    作者:Tyler Milston Renner, Kasandra Bélanger and Marc-André Langlois日期:09/05/2018,浏览量:432,Q&A: 0
    [Abstract] There exists a wide variety of techniques to isolate and purify viral particles from cell culture supernatants. However, these techniques vary greatly in ease of use, purity, yield and impact on viral structural integrity. Most importantly, it is becoming evident that secreted extracellular vesicles (EVs) co-purify with retroviruses using nearly ...