病毒

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    现刊
    Enterovirus Competition Assay to Assess Replication Fitness
    用于检测复制适应性的肠道病毒竞争实验
    作者:Valeria Lulla and Andrew E. Firth日期:05/20/2019,浏览量:133,Q&A: 0
    In virology the difference between the fitness of two viruses can be determined by using various methods, such as virus titer, growth curve analysis, measurement of virus infectivity, analysis of produced RNA copies and viral protein production. ...
    TZA, a Sensitive Reporter Cell-based Assay to Accurately and Rapidly Quantify Inducible, Replication-competent Latent HIV-1 from Resting CD4+ T Cells
    TZA, 一种基于敏感报告因子细胞学检测方法, 可用于准确快速量化静息CD4+ T细胞中可诱导的且复制能力强的潜伏HV-1
    作者:Anwesha Sanyal, Vatsala S. Rangachar and Phalguni Gupta日期:05/20/2019,浏览量:168,Q&A: 0
    The latent HIV-1 viral reservoir in resting CD4+ (rCD4+) T cells represents a major barrier to an HIV-1 cure. There is an ongoing effort to identify therapeutic approaches that will eliminate or reduce the size of this ...
    Purification and Proteomic Analysis of Alphavirus Particles from Sindbis Virus Grown in Mammalian and Insect Cells
    哺乳动物和昆虫细胞中的辛德比斯病毒的α病毒颗粒的纯化和蛋白质组分析
    作者:Raquel Hernandez, Trevor Glaros, Gabrielle Rizzo and Davis F. Ferreira日期:05/20/2019,浏览量:126,Q&A: 0
    Current mass spectrometry (MS) methods and new instrumentation now allow for more accurate identification of proteins in low abundance than previous protein fractionation and identification methods. It was of interest if this method could serve to ...
    A Flow Cytometric Method to Determine Transfection Efficiency
    利用流式细胞术测定转染效率
    Mammalian cell transfection is a powerful technique commonly used in molecular biology to express exogenous DNA or RNA in cells and study gene and protein function. Although several transfection strategies have been developed, there is a wide ...
    Enterovirus Competition Assay to Assess Replication Fitness
    用于检测复制适应性的肠道病毒竞争实验
    作者:Valeria Lulla and Andrew E. Firth日期:05/20/2019,浏览量:133,Q&A: 0
    [Abstract] In virology the difference between the fitness of two viruses can be determined by using various methods, such as virus titer, growth curve analysis, measurement of virus infectivity, analysis of produced RNA copies and viral protein production. However, for closely performing viruses, it is often very hard to distinguish the differences. In vitro ...
    TZA, a Sensitive Reporter Cell-based Assay to Accurately and Rapidly Quantify Inducible, Replication-competent Latent HIV-1 from Resting CD4+ T Cells
    TZA, 一种基于敏感报告因子细胞学检测方法, 可用于准确快速量化静息CD4+ T细胞中可诱导的且复制能力强的潜伏HV-1
    作者:Anwesha Sanyal, Vatsala S. Rangachar and Phalguni Gupta日期:05/20/2019,浏览量:168,Q&A: 0
    [Abstract] The latent HIV-1 viral reservoir in resting CD4+ (rCD4+) T cells represents a major barrier to an HIV-1 cure. There is an ongoing effort to identify therapeutic approaches that will eliminate or reduce the size of this reservoir. However, clinical investigators lack an assay to determine whether or not a decrease in the ...
    Purification and Proteomic Analysis of Alphavirus Particles from Sindbis Virus Grown in Mammalian and Insect Cells
    哺乳动物和昆虫细胞中的辛德比斯病毒的α病毒颗粒的纯化和蛋白质组分析
    作者:Raquel Hernandez, Trevor Glaros, Gabrielle Rizzo and Davis F. Ferreira日期:05/20/2019,浏览量:126,Q&A: 0
    [Abstract] Current mass spectrometry (MS) methods and new instrumentation now allow for more accurate identification of proteins in low abundance than previous protein fractionation and identification methods. It was of interest if this method could serve to define the virus proteome of a membrane-containing virus. To evaluate the efficacy of mass spec to ...
    A Flow Cytometric Method to Determine Transfection Efficiency
    利用流式细胞术测定转染效率
    [Abstract] Mammalian cell transfection is a powerful technique commonly used in molecular biology to express exogenous DNA or RNA in cells and study gene and protein function. Although several transfection strategies have been developed, there is a wide variation with regards to transfection efficiency, cell toxicity and reproducibility. Thus, a sensitive ...
    Unbiased Screening of Activated Receptor Tyrosine Kinases (RTKs) in Tumor Extracts Using a Mouse Phospho-RTK Array Kit
    利用小鼠Phospho-RTK Array试剂盒无偏筛选肿瘤提取物中激活受体酪氨酸激酶
    作者:Julian Naipauer, Lucas E. Cavallin and Enrique A. Mesri日期:04/20/2019,浏览量:375,Q&A: 0
    [Abstract] Kaposi’s sarcoma (KS) herpesvirus (KSHV) is a virus that causes KS, an angiogenic AIDS-associated spindle-cell neoplasm, by activating host oncogenic signaling cascades through autocrine and paracrine mechanisms. Many host signaling cascades co-opted by KSHV including PI3K/AKT/mTORC, NFkB and Notch are critical for cell-specific mechanisms of ...
    Quantification of Hepatitis B Virus Covalently Closed Circular DNA in Infected Cell Culture Models by Quantitative PCR
    qPCR定量分析感染细胞培养模型中乙型肝炎病毒共价闭合环状DNA
    作者:Bingqian Qu and Stephan Urban日期:04/05/2019,浏览量:489,Q&A: 0
    [Abstract] Persistence of the human hepatitis B virus (HBV) requires the maintenance of covalently closed circular (ccc)DNA, the episomal genome reservoir in nuclei of infected hepatocytes. cccDNA elimination is a major aim in future curative therapies currently under development. In cell culture based in vitro studies, both hybridization- and ...
    Viral Chromosome Conformation Capture (V3C) Assays for Identifying Trans-interaction Sites between Lytic Viruses and the Cellular Genome
    病毒染色体构象俘获技术用于裂解病毒和细胞基因组之间反向相互作用位点的检测
    作者:Kinjal Majumder, Maria Boftsi and David J Pintel日期:03/20/2019,浏览量:476,Q&A: 0
    [Abstract] The folding mechanisms of the mammalian genome package our genetic material into the nucleus, and in doing so, dictate its appropriate replication and expression. Chromosome conformation capture technology has enabled the dissection of the folding principles of the cellular genome. This has led to a better understanding of the role played by ...
    On-demand Labeling of SNAP-tagged Viral Protein for Pulse-Chase Imaging, Quench-Pulse-Chase Imaging, and Nanoscopy-based Inspection of Cell Lysates
    用于脉冲追踪成像,猝发脉冲追踪成像和基于纳米显微技术的细胞裂解物检测的融合SNAP病毒蛋白的按需标记
    作者:Roland Remenyi, Raymond Li and Mark Harris日期:02/20/2019,浏览量:871,Q&A: 0
    [Abstract] Advanced labeling technologies allow researchers to study protein turnover inside intact cells and to track the labeled protein in downstream applications. In the context of a viral infection, the combination of imaging and fluorescent labeling of viral proteins sheds light on their biological activity and interaction with the host cell. Initial ...
    Analysis of Early Phase HIV-1 Replication and Integration Events by Using Real-time PCR
    利用实时PCR对早期HIV-1复制和整合过程进行检测
    作者:Graf Laura, Steven P. Moran, Sally Ro, Carlos M.C. de Noronha and Binshan Shi日期:02/20/2019,浏览量:607,Q&A: 0
    [Abstract] Upon entry into a host cell, the HIV-1 virus undergoes a series of critical early replication events including reverse transcription, nuclear import, and integration of its cDNA into the host genome. Molecular assays used to detect and analyze changes in HIV-1 early phase replication events are valuable tools in developing potential antiretroviral ...
    Production, Titration and Imaging of Zika Virus in Mammalian Cells
    哺乳动物细胞中寨卡病毒的制备、滴度测定和成像
    作者:Wesley Freppel, Clément Mazeaud and Laurent Chatel-Chaix日期:12/20/2018,浏览量:1200,Q&A: 0
    [Abstract] Since the outbreak of Zika virus (ZIKV) in Latin America and the US in 2016, this flavivirus has emerged as a major threat for public health. Indeed, it is now clear that ZIKV is vertically transmitted from the infected mother to the fetus and this may lead to severe neurological development defects including (but not restricted to) neonate ...