植物科学

分类

    现刊
    Micrografting in Arabidopsis Using a Silicone Chip
    利用硅芯片进行拟南芥微嫁接
    作者:Hiroki Tsutsui, Yaichi Kawakatsu and Michitaka Notaguchi日期:06/20/2021,浏览量:390,Q&A: 0

    The micrografting technique in the model plant Arabidopsis has been widely used in the field of plant science. Grafting experiments have demonstrated that signal transductions are systematically regulated in many plant characteristics, including

    ...
    Peptide-mediated Targeting of Nanoparticles with Chemical Cargoes to Chloroplasts in Arabidopsis Plants
    多肽介导的化学物质纳米粒对拟南芥叶绿体的靶向作用
    作者:Israel Santana, Peiguang Hu, Su-Ji Jeon, Chris Castillo, Hann Tu and Juan Pablo Giraldo日期:06/20/2021,浏览量:562,Q&A: 0

    Plant nanobiotechnology is a flourishing field that uses nanomaterials to study and engineer plant function. Applications of nanotechnology in plants have great potential as tools for improving crop yield, tolerance to disease and environmental

    ...
    Micrografting in Arabidopsis Using a Silicone Chip
    利用硅芯片进行拟南芥微嫁接
    作者:Hiroki Tsutsui, Yaichi Kawakatsu and Michitaka Notaguchi日期:06/20/2021,浏览量:390,Q&A: 0
    [Abstract]

    The micrografting technique in the model plant Arabidopsis has been widely used in the field of plant science. Grafting experiments have demonstrated that signal transductions are systematically regulated in many plant characteristics, including defense mechanisms and responses to surrounding environments such as soil and light conditions.

    ...
    Peptide-mediated Targeting of Nanoparticles with Chemical Cargoes to Chloroplasts in Arabidopsis Plants
    多肽介导的化学物质纳米粒对拟南芥叶绿体的靶向作用
    作者:Israel Santana, Peiguang Hu, Su-Ji Jeon, Chris Castillo, Hann Tu and Juan Pablo Giraldo日期:06/20/2021,浏览量:562,Q&A: 0
    [Abstract]

    Plant nanobiotechnology is a flourishing field that uses nanomaterials to study and engineer plant function. Applications of nanotechnology in plants have great potential as tools for improving crop yield, tolerance to disease and environmental stress, agrochemical delivery of pesticides and fertilizers, and genetic modification and transformation

    ...
    Rice Root Hair Phenotypes Imaged by Cryo-SEM
    水稻根毛表型的低温扫描电镜成像
    [Abstract]

    Cryo-scanning electron microscopy (cryo-SEM) was first introduced for scientific use in the 1980s. Since then, cryo-SEM has become a routine technique for studying the surfaces and internal structures of biological samples with a high water content. In contrast to traditional SEM, cryo-SEM requires no sample pretreatment processes; thus, we can

    ...
    Age, Wound Size and Position of Injury – Dependent Vascular Regeneration Assay in Growing Leaves
    年龄、伤口大小和损伤部位 – 生长叶片中依赖于损伤的维管再生试验
    [Abstract]

    Recurring damage to the aerial organs of plants necessitates their prompt repair, particularly their vasculature. While vascular regeneration assays for aerial plant parts such as the stem and inflorescence stalk are well established, those for leaf vasculature remain unexplored. Recently, we established a new vascular regeneration assay in

    ...
    Histological Methods to Detect Early-stage Plant Defense Responses during Artificial Inoculation of Lolium perenne with Epichloë festucae
    羊茅人工接种多年生黑麦草过程中植物早期防御反应的组织学检测
    作者:Mostafa Rahnama, Damien J. Fleetwood and Richard D. Johnson日期:05/05/2021,浏览量:803,Q&A: 0
    [Abstract]

    Epichloë species form agriculturally important symbioses with many cool season grasses. To study these symbioses, such as the interaction of Epichloë festucae with perennial ryegrass (Lolium perenne), host plants can be infected by artificial inoculation of etiolated seedlings. This inoculation is performed by placing mycelium into an

    ...
    Characterising Plant Deubiquitinases with in vitro Activity-based Labelling and Ubiquitin Chain Disassembly Assays
    植物去泛素酶的体外活性标记和泛素链分解分析
    作者:Michael J. Skelly and Steven H. Spoel日期:05/05/2021,浏览量:689,Q&A: 0
    [Abstract]

    Post-translational modification of proteins by ubiquitin is an essential cellular signaling mechanism in all eukaryotes. Ubiquitin is removed from target proteins by a wide range of deubiquitinase (DUB) enzymes with different activities and substrate specificities. Understanding how DUBs function in vitro is a vital first step to uncovering their

    ...
    In vitro Reconstitution Assays of Arabidopsis 20S Proteasome
    拟南芥20S蛋白酶体的体外重组分析
    作者:Yanjun Li, Di Sun, Xingxing Yan, Zhiye Wang and Xiuren Zhang日期:04/05/2021,浏览量:855,Q&A: 0
    [Abstract]

    The majority of cellular proteins are degraded by the 26S proteasome in eukaryotes. However, intrinsically disordered proteins (IDPs), which contain large portions of unstructured regions and are inherently unstable, are degraded via the ubiquitin-independent 20S proteasome. Emerging evidence indicates that plant IDP homeostasis may also be

    ...
    Atomic Force Microscopy to Characterize Ginger Lipid-Derived Nanoparticles (GLDNP)
    原子力显微镜研究生姜脂质纳米颗粒(GLDNP)
    作者:Dingpei Long, Chunhua Yang, Junsik Sung and Didier Merlin日期:04/05/2021,浏览量:871,Q&A: 0
    [Abstract]

    We have demonstrated that a specific population of ginger-derived nanoparticles (GDNP-2) could effectively target the colon, reduce colitis, and alleviate colitis-associated colon cancer. Naturally occurring GDNP-2 contains complex bioactive components, including lipids, proteins, miRNAs, and ginger secondary metabolites (gingerols and shogaols).

    ...
    A Novel Method to Construct Binary CRISPR Vectors for Plant Transformation by Single Round of PCR Amplification
    一种通过单轮PCR扩增构建用于植物转化的二元CRISPR载体的新方法
    作者:Kang Li, Yuhui Wang and Chuanying Fang日期:04/05/2021,浏览量:987,Q&A: 0
    [Abstract]

    CRISPR/Cas9 is an established and flexible tool for genome editing. However, most methods used to generate expression clones for the CRISPR/Cas9 are time-consuming. Hence, we have developed a one-step protocol to introduce sgRNA expression cassette(s) directly into binary vectors (Liu et al., 2020). In this approach, we have optimized the

    ...
    Measurements of Root Colonized Bacteria Species
    根定植细菌种类的测量
    作者:Vílchez Juan Ignacio, Yu Yang, Dian Yi and Huiming Zhang日期:04/05/2021,浏览量:1024,Q&A: 0
    [Abstract]

    Root-associated bacteria are able to influence plant fitness and vigor. A key step in understanding the belowground plant-bacteria interactions is to quantify root colonization by the bacteria of interest. Probably, genetic engineering with fluorescence markers is the most powerful way to monitor bacterial strains in plant. However, this could

    ...