植物科学

分类

    现刊
    Isolation of Thylakoid Membranes from the Cyanobacterium Synechocystis sp. PCC 6803 and Analysis of Their Photosynthetic Pigment-protein Complexes by Clear Native-PAGE
    从蓝藻集胞藻sp. PCC 6803中分离类囊体膜并利用CN-PAGE非变性聚丙烯酰胺凝胶电泳分析光合色素蛋白复合物
    作者:Josef Komenda, Vendula Krynická and Tomas Zakar日期:01/05/2019,浏览量:144,Q&A: 0
    Cyanobacteria represent a frequently used model organism for the study of oxygenic photosynthesis. They belong to prokaryotic microorganisms but their photosynthetic apparatus is quite similar to that found in algal and plant chloroplasts. The key ...
    Isolation of Thylakoid Membranes from the Cyanobacterium Synechocystis sp. PCC 6803 and Analysis of Their Photosynthetic Pigment-protein Complexes by Clear Native-PAGE
    从蓝藻集胞藻sp. PCC 6803中分离类囊体膜并利用CN-PAGE非变性聚丙烯酰胺凝胶电泳分析光合色素蛋白复合物
    作者:Josef Komenda, Vendula Krynická and Tomas Zakar日期:01/05/2019,浏览量:144,Q&A: 0
    [Abstract] Cyanobacteria represent a frequently used model organism for the study of oxygenic photosynthesis. They belong to prokaryotic microorganisms but their photosynthetic apparatus is quite similar to that found in algal and plant chloroplasts. The key players in light reactions of photosynthesis are Photosystem I and Photosystem II complexes (PSI and ...
    Visualization and Quantification of Cell-to-cell Movement of Proteins in Nicotiana benthamiana
    本氏烟中蛋白的细胞间运动的可视化定量检测
    作者:Mila C Blekemolen, Mariliis Tark-Dame and Frank L.W Takken日期:12/20/2018,浏览量:437,Q&A: 0
    [Abstract] Cell-to-cell movement of proteins through plasmodesmata is a widely-established mechanism for intercellular signaling in plants. Current techniques to study intercellular protein translocation rely on single-cell transformation using particle bombardment or transgenic lines expressing photo-inducible fluorophores. The method presented here allows ...
    Genome-wide Estimation of Evolutionary Distance and Phylogenetic Analysis of Homologous Genes
    进化距离的全基因组评估和同源基因的系统进化分析
    作者:Meixia Zhao, Biao Zhang, Jianxin Ma and Damon Lisch日期:12/05/2018,浏览量:344,Q&A: 0
    [Abstract] Homologous genes, including paralogs and orthologs, are genes that share sequence homologies within or between different species. Homologous genes originate from a common origin through speciation, genetic duplication or horizontal gene transfer. Estimation of the sequence divergence of homologous genes help us to understand divergence time, which ...
    Using Arabidopsis Mesophyll Protoplasts to Study Unfolded Protein Response Signaling
    利用拟南芥叶肉细胞原生质体研究未折叠蛋白响应信号
    作者:Yan Bao and Diane C. Bassham日期:12/05/2018,浏览量:368,Q&A: 0
    [Abstract] Various environmental stresses or artificial reagents can trigger unfolded protein accumulation in the endoplasmic reticulum (ER) due to the folding capacity of the ER being exceeded. This is termed ER stress, and triggers the unfolded protein response (UPR). Assays for activation of the UPR in plants include Tunicamycin (Tm)- or dithiothreitol ...
    An Innovative Approach to Study Ralstonia solanacearum Pathogenicity in 6 to 7 Days Old Tomato Seedlings by Root Dip Inoculation
    一种通过根部浸渍接种研究6-7天番茄幼苗青枯雷尔氏菌致病性的新方法
    作者:Niraj Singh, Rahul Kumar and Suvendra Kumar Ray日期:11/05/2018,浏览量:443,Q&A: 0
    [Abstract] Ralstonia solanacearum (F1C1) is a Gram-negative plant pathogenic bacterium that causes lethal wilt disease in a wide range of plant species. This pathogen is very well known for its unpredictable behavior during infection and wilting its host. Because of its mysterious infection behavior, virulence and pathogenicity standardization are ...
    Rice Ragged Stunt Virus Propagation and Infection on Rice Plants
    稻株中水稻齿叶矮缩病毒的传播和感染
    作者:Chao Zhang, Chaonan Shi, Dong Chen and Jianguo Wu日期:10/20/2018,浏览量:377,Q&A: 0
    [Abstract] Virus inoculation is a basic experimental procedure to evaluate the resistance of a rice variety or a transgenic material upon virus infection. We recently demonstrated that Rice Ragged Stunt Virus (RRSV), an oryzavirus that is transmitted by brown planthopper (BPH), can suppress jasmonic acid-mediated antiviral defense through ...
    Examining Autophagy in Plant by Transmission Electron Microscopy (TEM)
    利用透射电镜检测植物细胞自噬
    作者:Xiyin Zheng, Chenguang Zhao and Yule Liu日期:10/20/2018,浏览量:481,Q&A: 0
    [Abstract] In plants, macroautophagy, here referred as autophagy, is a degradation pathway during which the double-membrane structure named autophagosome engulfs the cargo and then fuses with vacuole for material recycling.

    To investigate the process of autophagy, transmission electron microscopy (TEM) was used to monitor the ultrastructure of ...
    Pneumatic Method to Measure Plant Xylem Embolism
    气动法检测植物木质部栓塞
    作者:Paulo R. L. Bittencourt, Luciano Pereira and Rafael S. Oliveira日期:10/20/2018,浏览量:435,Q&A: 0
    [Abstract] Embolism, the formation of air bubbles in the plant water transport system, has a major impact on plant water relations. Embolism formation in the water transport system of plants disrupts plant water transport capacity, impairing plant functioning and triggering plant mortality. Measuring embolism with traditional hydraulic methods is both ...
    Real-time PCR Analysis of PAMP-induced Marker Gene Expression in Nicotiana benthamiana
    本塞姆氏烟草中PAMP诱导标记基因表达的实时定量PCR分析
    作者:Fan Liu, Yuanpeng Xu, Yan Wang and Yuanchao Wang日期:10/05/2018,浏览量:649,Q&A: 0
    [Abstract] Perception of pathogen-associated molecular patterns (PAMPs) often triggers various innate immune responses in plants. The transcriptional changes of defense-related genes are often used as a marker to assay PAMP-triggered plant immune response. Here we described a protocol to monitor the relative expression level of marker genes in Nicotiana ...
    An Image Analysis Pipeline to Quantify Emerging Cracks in Materials or Adhesion Defects in Living Tissues
    分析活体组织中出现的材料裂缝或附着力缺陷的图像分析法
    作者:Stéphane Verger, Guillaume Cerutti and Olivier Hamant日期:10/05/2018,浏览量:432,Q&A: 0
    [Abstract] Microcracks in materials reflect their mechanical properties. The quantification of the number or orientation of such cracks is thus essential in many fields, including engineering and geology. In biology, cracks in soft tissues can reflect adhesion defects, and the analysis of their pattern can help to deduce the magnitude and orientation of ...