植物科学

分类

    现刊
    In vitro Assessment of Pathogen Effector Binding to Host Proteins by Surface Plasmon Resonance
    利用表面等离子体共振技术评价病原菌与宿主蛋白的结合
    作者:Marina Franceschetti, Mark J. Banfield, Clare E. M. Stevenson and Juan Carlos De la Concepcion日期:07/05/2020,浏览量:19,Q&A: 0
    The mechanisms of virulence and immunity are often governed by molecular interactions between pathogens and host proteins. The study of these interactions has major implications on understanding virulence activities, and how the host immune system ...
    Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids
    利用甲基化敏感扩增多态性分析野生马铃薯杂交种
    作者:Nicolás Cara, Carlos F. Marfil, María V. Bertoldi and Ricardo W. Masuelli日期:07/05/2020,浏览量:12,Q&A: 0
    Methylation-Sensitive Amplification Polymorphism (MSAP) is a versatile marker for analyzing DNA methylation patterns in non-model species. The implementation of this technique does not require a reference genome and makes it possible to determine ...
    In vitro Assessment of Pathogen Effector Binding to Host Proteins by Surface Plasmon Resonance
    利用表面等离子体共振技术评价病原菌与宿主蛋白的结合
    作者:Marina Franceschetti, Mark J. Banfield, Clare E. M. Stevenson and Juan Carlos De la Concepcion日期:07/05/2020,浏览量:19,Q&A: 0
    [Abstract] The mechanisms of virulence and immunity are often governed by molecular interactions between pathogens and host proteins. The study of these interactions has major implications on understanding virulence activities, and how the host immune system recognizes the presence of pathogens to initiate an immune response. Frequently, the association ...
    Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids
    利用甲基化敏感扩增多态性分析野生马铃薯杂交种
    作者:Nicolás Cara, Carlos F. Marfil, María V. Bertoldi and Ricardo W. Masuelli日期:07/05/2020,浏览量:12,Q&A: 0
    [Abstract] Methylation-Sensitive Amplification Polymorphism (MSAP) is a versatile marker for analyzing DNA methylation patterns in non-model species. The implementation of this technique does not require a reference genome and makes it possible to determine the methylation status of hundreds of anonymous loci distributed throughout the genome. In addition, ...
    Maintenance and Quantitative Phenotyping of the Oomycete-plant Model Pathosystem Hyaloperonospora arabidopsidisArabidopsis
    卵菌-植物模型病理系统(活体营养型卵菌–拟南芥)的维持和定量表型分析
    作者:Fang-Yu Hwu and Martin Parniske日期:06/20/2020,浏览量:448,Q&A: 0
    [Abstract] The interaction between the host plant Arabidopsis thaliana (Arabidopsis) and the oomycete Hyaloperonospora arabidopsidis (Hpa) is an established model system for the study of an obligate biotrophic downy mildew interaction. The evaluation of the developmental success of Hpa is often based on the quantification ...
    Determination of Ureides Content in Plant Tissues
    植物组织中酰脲含量的测定
    作者:Ignacio Lescano日期:06/05/2020,浏览量:579,Q&A: 0
    [Abstract] The ureides allantoin and allantoate are the main organic nitrogen compounds transported in several legumes, predominantly from N2 fixation. Moreover, recent studies point out a remarkable role for allantoin during several stress responses of plants other than legumes. The goal of this protocol is to determine ureides concentration in ...
    Safe DNA-extraction Protocol Suitable for Studying Tree-fungus Interactions
    适于研究树-真菌互作的安全DNA提取方法
    作者:Susanna Keriö, Eeva Terhonen and Jared M. LeBoldus日期:06/05/2020,浏览量:960,Q&A: 0
    [Abstract] We present a safe and low-cost method suitable for DNA extraction from mycelium and tree tissue samples. After sample preparation, the extraction takes about 60 min. Method performance was tested by extracting DNA from various tree tissue samples and from mycelium grown on solid and liquid media. DNA was extracted from juvenile and mature host ...
    Purification of Protein-complexes from the Cyanobacterium Synechocystis sp. PCC 6803 Using FLAG-affinity Chromatography
    Cyanobacterium Synechocystis sp. PCC 6803 中蛋白混合物的标记亲和层析纯化方法
    作者:Minna M. Koskela, Petra Skotnicová, Éva Kiss and Roman Sobotka日期:05/20/2020,浏览量:602,Q&A: 0
    [Abstract] Exploring the structure and function of protein complexes requires their isolation in the native state–a task that is made challenging when studying labile and/or low abundant complexes. The difficulties in preparing membrane-protein complexes are especially notorious. The cyanobacterium Synechocystis sp. PCC 6803 is a widely used model ...
    Live Cell Imaging of Male Meiosis in Arabidopsis by a Landmark-based System
    地标系统在拟南芥雄性减数分裂活体细胞成像中的应用
    作者:Maria Ada Prusicki, Emma Mathilde Keizer, Rik Peter van Rosmalen, Christian Fleck and Arp Schnittger日期:05/05/2020,浏览量:1018,Q&A: 0
    [Abstract] Live cell imaging has tremendously promoted our understanding of cellular and subcellular processes such as cell division. Here, we present a step-by-step protocol for a robust and easy-to-use live cell imaging approach to study male meiosis in the plant Arabidopsis thaliana as recently established. Our method relies on the concomitant ...
    MAMP-triggered Medium Alkalinization of Plant Cell Cultures
    MAMP诱导植物细胞培养基质碱化
    [Abstract] Plants recognize a wide variety of microbial molecules to detect and respond to potential invaders. Recognition of Microbe-Associated Molecular Patterns (MAMPs) by cell surface receptors initiate a cascade of biochemical responses that include, among others, ion fluxes across the plasma membrane. A consequence of such event is a decrease in the ...
    Accelerated Snowmelt Protocol to Simulate Climate Change Induced Impacts on Snowpack Dependent Ecosystems
    加速雪融诱发气候变化对依赖雪被斑块生态系统的影响
    作者:Laura T. Leonard, Chelsea Wilmer, Heidi Steltzer, Kenneth H. Williams and Jonathan O. Sharp日期:03/20/2020,浏览量:927,Q&A: 0
    [Abstract] Field studies that simulate the effects of climate change are important for a predictive understanding of ecosystem responses to a changing environment. Among many concerns, regional warming can result in advanced timing of spring snowmelt in snowpack dependent ecosystems, which could lead to longer snow-free periods and drier summer soils. Past ...
    High-resolution and Deep Phylogenetic Reconstruction of Ancestral States from Large Transcriptomic Data Sets
    从大型转录组数据集中进行原始状态的高通量深度系统发育重建
    作者:Sumanth Kumar Mutte and Dolf Weijers日期:03/20/2020,浏览量:844,Q&A: 0
    [Abstract] Phylogenetics is an important area of evolutionary biology that helps to understand the origin and divergence of genes, genomes and species. Building meaningful phylogenetic trees is needed for the accurate reconstruction of the past. To achieve a correct phylogenetic understanding of genes or proteins, reliable and robust methods are needed to ...