生物化学

分类

    现刊
    Production and Crystallization of Nanobodies in Complex with the Receptor Binding Domain of the SARS-CoV-2 Spike Protein
    与 SARS-CoV-2 刺突蛋白受体结合域复合的纳米抗体的产生和结晶

    The receptor binding domain (RBD) of the spike protein of SARS-CoV-2 binds angiotensin converting enzyme-2 (ACE-2) on the surface of epithelial cells, leading to fusion, and entry of the virus into the cell. This interaction can be blocked by the

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    Translating Ribosome Affinity Purification (TRAP) of Cell Type-specific mRNA from Mouse Brain Lysates
    小鼠脑裂解物中细胞类型特异性 mRNA 的核糖体翻译亲和纯化 (TRAP)
    作者:Catherine L. Salussolia, Kellen D. Winden and Mustafa Sahin日期:05/05/2022,浏览量:400,Q&A: 0

    Mammalian tissues are highly heterogenous and complex, posing a challenge in understanding the molecular mechanisms regulating protein expression within various tissues. Recent studies have shown that translation at the level of the ribosome is

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    Immunoisolation of Endosomal Recycling Vesicles from Saccharomyces cerevisiae
    酿酒酵母内体循环囊泡的免疫分离
    作者:Sho W. Suzuki and Scott D. Emr日期:05/05/2022,浏览量:412,Q&A: 0

    Endosomal recycling is essential for the appropriate function of the endosome. During this process, endosomal coat complexes (i.e., retromer, and Mvp1) are recruited to the endosome, and deform its membrane to form recycling vesicles. To further

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    Expression, Purification, and in vitro Enzyme Activity Assay of a Recombinant Aldehyde Dehydrogenase from Thermus thermophilus, using an Escherichia coli host
    使用大肠杆菌宿主的嗜热栖热菌重组醛脱氢酶的表达、纯化和体外酶活性测定
    作者:Kim Shortall, Edmond Magner and Tewfik Soulimane日期:05/05/2022,浏览量:523,Q&A: 0

    Based on previous in-depth characterisation, aldehyde dehydrogenases (ALDH) are a diverse superfamily of enzymes, in terms of both structure and function, present in all kingdoms of life. They catalyse the oxidation of an aldehyde to carboxylic acid

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    Production and Crystallization of Nanobodies in Complex with the Receptor Binding Domain of the SARS-CoV-2 Spike Protein
    与 SARS-CoV-2 刺突蛋白受体结合域复合的纳米抗体的产生和结晶
    [Abstract]

    The receptor binding domain (RBD) of the spike protein of SARS-CoV-2 binds angiotensin converting enzyme-2 (ACE-2) on the surface of epithelial cells, leading to fusion, and entry of the virus into the cell. This interaction can be blocked by the binding of llama-derived nanobodies (VHHs) to the RBD, leading to virus neutralisation. Structural

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    Translating Ribosome Affinity Purification (TRAP) of Cell Type-specific mRNA from Mouse Brain Lysates
    小鼠脑裂解物中细胞类型特异性 mRNA 的核糖体翻译亲和纯化 (TRAP)
    作者:Catherine L. Salussolia, Kellen D. Winden and Mustafa Sahin日期:05/05/2022,浏览量:400,Q&A: 0
    [Abstract]

    Mammalian tissues are highly heterogenous and complex, posing a challenge in understanding the molecular mechanisms regulating protein expression within various tissues. Recent studies have shown that translation at the level of the ribosome is highly regulated, and can vary independently of gene expression observed at a transcriptome level, as

    ...
    Immunoisolation of Endosomal Recycling Vesicles from Saccharomyces cerevisiae
    酿酒酵母内体循环囊泡的免疫分离
    作者:Sho W. Suzuki and Scott D. Emr日期:05/05/2022,浏览量:412,Q&A: 0
    [Abstract]

    Endosomal recycling is essential for the appropriate function of the endosome. During this process, endosomal coat complexes (i.e., retromer, and Mvp1) are recruited to the endosome, and deform its membrane to form recycling vesicles. To further analyze this, we developed a protocol for the immunoisolation of recycling vesicles from budding yeast.

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    Expression, Purification, and in vitro Enzyme Activity Assay of a Recombinant Aldehyde Dehydrogenase from Thermus thermophilus, using an Escherichia coli host
    使用大肠杆菌宿主的嗜热栖热菌重组醛脱氢酶的表达、纯化和体外酶活性测定
    作者:Kim Shortall, Edmond Magner and Tewfik Soulimane日期:05/05/2022,浏览量:523,Q&A: 0
    [Abstract]

    Based on previous in-depth characterisation, aldehyde dehydrogenases (ALDH) are a diverse superfamily of enzymes, in terms of both structure and function, present in all kingdoms of life. They catalyse the oxidation of an aldehyde to carboxylic acid using the cofactor nicotinamide adenine dinucleotide (phosphate) (NAD(P)+), and are often not

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    Apoplastic Expression of CARD1-ecto Domain in Nicotiana benthamiana and Purification from the Apoplastic Fluids
    本氏烟草中 CARD1-ecto 结构域的质外体表达及质外体液的纯化
    作者:Nobuaki Ishihama, Anuphon Laohavisit, Kaori Takizawa and Ken Shirasu日期:04/20/2022,浏览量:740,Q&A: 0
    [Abstract]

    The protein expression and purification process is an essential initial step for biochemical analysis of a protein of interest. Traditionally, heterologous protein expression systems (such as E. coli, yeast, insect cells, and cell-free) are employed for plant protein expression, although a plant expression system is often desirable for plant

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    Visualization and Purification of Caenorhabditis elegans Germ Granule Proteins Using Proximity Labeling
    利用近似标记可视化和纯化秀丽隐杆线虫的胚芽颗粒蛋白
    作者:Hannah L. Hertz, Ian F. Price and Wen Tang日期:04/20/2022,浏览量:1023,Q&A: 0
    [Abstract]

    Membraneless organelles, such as germ granules and stress granules, are liquid-like condensates formed by phase transition. Recently, we and others have adopted proximity-based labeling methods to determine the composition of these membraneless compartments. Here, we describe the use of TurboID—an engineered promiscuous biotin

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    Bsoft: Image Processing for Structural Biology
    Bsoft:结构生物学的图像处理
    作者:J. Bernard Heymann日期:04/20/2022,浏览量:1042,Q&A: 0
    [Abstract]

    Bsoft is a software package primarily developed for processing electron micrographs, with the goal of determining the structures of biologically relevant molecules, molecular assemblies, and parts of cells. However, it incorporates many ways to deal with images, from the mundane to very sophisticated algorithms. This article is an introduction

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    A SYBR Gold-based Label-free in vitro Dicing Assay
    基于 SYBR Gold 的无标记体外切割试验
    [Abstract]

    In Arabidopsis, DICER-LIKE PROTEIN 3 (DCL3) cuts the substrate pre-siRNA into a product siRNA duplex, encompassing one 23-nt strand and one 24-nt strand. To monitor the separation of the siRNA duplex with only 1-nt difference, we developed this protocol to evaluate the in vitro dicing activity of DCL3. The method can be applied for measuring

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    In vitro Auto- and Substrate-Ubiquitination Assays
    体外自体和底物泛素化测定
    作者:Hye Lin Park, Han Yong Lee and Gyeong Mee Yoon日期:04/05/2022,浏览量:423,Q&A: 0
    [Abstract]

    The precise regulation of the homeostasis of the cellular proteome is critical for the appropriate growth and development of plants. It also allows the plants to respond to various environmental stresses, by modulating their biochemical and physiological aspects in a timely manner. Ubiquitination of cellular proteins is one of the major

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    Tracking the Reversed Oxidative Tricarboxylic Acid Cycle in Bacteria
    追踪细菌中的反向氧化三羧酸循环
    作者:Lydia Steffens, Eugenio Pettinato, Thomas M. Steiner, Wolfgang Eisenreich and Ivan A. Berg日期:03/20/2022,浏览量:787,Q&A: 1
    [Abstract]

    Different pathways for autotrophic CO2 fixation can be recognized by the presence of genes for their specific key enzymes. On this basis, (meta)genomic, (meta)transcriptomic, or (meta)proteomic analysis enables the identification of the role of an organism or a distinct pathway in primary production. However, the recently discovered variant of the

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