其它脊椎动物

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    现刊
    Microplastic Extraction from Marine Vertebrate Digestive Tracts, Regurgitates and Scats: A Protocol for Researchers from All Experience Levels
    海洋脊椎动物消化道,反流物和粪便中塑料微粒的提取:一个适用于所有经验水平研究者的实验方法
    作者:Amy L. Lusher and Gema Hernandez-Milian日期:11/20/2018,浏览量:589,Q&A: 0
    [Abstract] It is essential to provide a protocol for the separation and identification of microplastics in marine vertebrates (mammals, birds, turtles and fish) that is easy to follow and adaptable depending on research infrastructure. Digesting organic material is an effective way to analyze samples for microplastics. Presented here is an optimized protocol ...
    Studying the Mechanisms of Developmental Vocal Learning and Adult Vocal Performance in Zebra Finches through Lentiviral Injection
    通过慢病毒注射研究斑马雀的发育声乐学习和成年声乐表演的机制
    作者:Zhimin Shi, Ofer Tchernichovski and XiaoChing Li日期:09/05/2018,浏览量:473,Q&A: 0
    [Abstract] Here we provide a detailed step-by-step protocol for using lentivirus to manipulate miRNA expression in Area X of juvenile zebra finches and for analyzing the consequences on song learning and song performance. This protocol has four parts: 1) making the lentiviral construct to overexpress miRNA miR-9; 2) packaging the lentiviral vector; 3) ...
    Xenopus laevis Oocytes Preparation for in-Cell EPR Spectroscopy
    制备非洲爪蟾卵母细胞用于细胞内EPR光谱学分析
    作者:Laura John and Malte Drescher日期:04/05/2018,浏览量:1288,Q&A: 0
    [Abstract] One of the most exciting perspectives for studying bio-macromolecules comes from the emerging field of in-cell spectroscopy, which enables to determine the structure and dynamics of bio-macromolecules in the cell. In-cell electron paramagnetic resonance (EPR) spectroscopy in combination with micro-injection of bio-macromolecules into Xenopus ...
    Boron Uptake Assay in Xenopus laevis Oocytes
    在非洲爪蟾卵母细胞系统中进行硼的吸收分析实验
    作者:Sheliang Wang, Namiki Mitani-Ueno and Junpei Takano日期:03/05/2018,浏览量:1429,Q&A: 0
    [Abstract] Boron (B) is essential for plant growth and taken up by plant roots as boric acid. Under B limitation, B uptake and translocation in plants are dependent on the boric acid channels located in the plasma membrane. Xenopus leavis oocyte is a reliable heterologous expression system to characterize transport activities of boric acid channels ...
    In vitro Engineered DNA-binding Molecule-mediated Chromatin Immunoprecipitation (in vitro enChIP) Using CRISPR Ribonucleoproteins in Combination with Next-generation Sequencing (in vitro enChIP-Seq) for the Identification of Chromosomal Interactions
    使用CRISPR核糖核蛋白的体外工程DNA结合分子介导的染色质免疫沉淀(体外enChIP)结合下一代测序(体外enChIP-Seq)用于染色体相互作用的鉴定
    作者:Toshitsugu Fujita and Hodaka Fujii日期:11/20/2017,浏览量:2878,Q&A: 0
    [Abstract] We have developed locus-specific chromatin immunoprecipitation (locus-specific ChIP) technologies consisting of insertional ChIP (iChIP) and engineered DNA-binding molecule-mediated ChIP (enChIP). Locus-specific ChIP is a method to isolate a genomic region of interest from cells while it also identifies what binds to this region using mass ...
    Preparation of the Partially Methylated Alditol Acetates Derived from CS Tetrasaccharides Containing Galactose for the Gas Chromatography/Mass Spectrometry Analysis
    用于气相色谱/质谱分析的含半乳糖CS四糖衍生的部分甲基化糖醇乙酸酯的制备
    作者:Kyohei Higashi and Toshihiko Toida日期:11/05/2017,浏览量:2397,Q&A: 0
    [Abstract] Chondroitin sulfate (CS), a member of the glycosaminoglycan (GAG) family of carbohydrates, is composed of linear, sulfated repeating disaccharide sequences of N-acetyl-D-galactosamine (GalNAc) and glucuronic acid (GlcA). Recently, a keratan sulfate (KS) disaccharide [GlcNAc6S(β1-3)Galactose(β1-]-branched CS-E was identified ...
    Uptake Assays in Xenopus laevis Oocytes Using Liquid Chromatography-mass Spectrometry to Detect Transport Activity
    非洲爪蟾卵母细胞联合液相色谱-质谱法的吸收实验检测转运活性
    作者:Morten Egevang Jørgensen, Christoph Crocoll, Barbara Ann Halkier and Hussam Hassan Nour-Eldin日期:10/20/2017,浏览量:2676,Q&A: 0
    [Abstract] Xenopus laevis oocytes are a widely used model system for characterization of heterologously expressed secondary active transporters. Historically, researchers have relied on detecting transport activity by measuring accumulation of radiolabeled substrates by scintillation counting or of fluorescently labelled substrates by ...
    Isolation of Keratan Sulfate Disaccharide-branched Chondroitin Sulfate E from Mactra chinensis
    中国蛤蜊中硫酸角质素二糖-支链硫酸软骨素E的分离
    作者:Kyohei Higashi and Toshihiko Toida日期:08/05/2017,浏览量:2293,Q&A: 0
    [Abstract] Glycosaminoglycans (GAGs) including chondroitin sulfate (CS), dermatan sulfate (DS), heparin (HP), heparan sulfate (HS) and keratan sulfate (KS) are linear, sulfated repeating disaccharide sequences containing hexosamine and uronic acid (or galactose in the case of KS). Recently, a keratan sulfate (KS) disaccharide [GlcNAc6S ...
    Spinal Cord Preparation from Adult Red-eared Turtles for Electrophysiological Recordings during Motor Activity
    制备成年红耳龟脊髓以用于运动活动期间电生理的记录
    作者:Peter C Petersen and Rune W Berg 日期:07/05/2017,浏览量:4261,Q&A: 0
    [Abstract] Although it is known that the generation of movements is performed to a large extent in neuronal circuits located in the spinal cord, the involved mechanisms are still unclear. The turtle as a model system for investigating spinal motor activity has advantages, which far exceeds those of model systems using other animals. The high resistance to ...
    Expression and Analysis of Flow-regulated Ion Channels in Xenopus Oocytes
    在非洲爪蟾卵母细胞中表达分析流量调节型的离子通道
    作者:Shujie Shi and Marcelo D. Carattino日期:04/20/2017,浏览量:3696,Q&A: 0
    [Abstract] Mechanically-gated ion channels play key roles in mechanotransduction, a process that translates physical forces into biological signals. Epithelial and endothelial cells are exposed to laminar shear stress (LSS), a tangential force exerted by flowing fluids against the wall of vessels and epithelia. The protocol outlined herein has been used to ...