发育生物学

分类

    现刊
    Visualization and Purification of Caenorhabditis elegans Germ Granule Proteins Using Proximity Labeling
    利用近似标记可视化和纯化秀丽隐杆线虫的胚芽颗粒蛋白
    作者:Hannah L. Hertz, Ian F. Price and Wen Tang日期:04/20/2022,浏览量:1023,Q&A: 0
    [Abstract]

    Membraneless organelles, such as germ granules and stress granules, are liquid-like condensates formed by phase transition. Recently, we and others have adopted proximity-based labeling methods to determine the composition of these membraneless compartments. Here, we describe the use of TurboID—an engineered promiscuous biotin

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    Isolation of tdTomato Expressing Inter-follicular Epidermal Melanocytes or Keratinocytes from Mouse Tail Skin
    从小鼠尾部皮肤中分离tdTomato表达滤泡间表皮黑色素细胞或角质形成细胞
    作者:Samuel Pop, Oscar Urtatiz and Catherine D. Van Raamsdonk日期:04/20/2022,浏览量:931,Q&A: 0
    [Abstract]

    The epidermis is the outermost layer of the skin. It is made up of mostly keratinocytes along with a small number of melanocytes and Langerhans cells. Melanocytes produce a pigment called melanin, which is transferred to the keratinocytes, and protects these cells from damage from UV radiation, as well as generating hair and skin colours. In this

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    RNA Interference Method for Gene Function Analysis in the Japanese Rhinoceros Beetle Trypoxylus dichotomus
    日本犀牛甲虫Trypoxylus dichotomus基因功能分析的RNA干扰方法
    作者:Kazuki Sakura, Shinichi Morita and Teruyuki Niimi日期:04/20/2022,浏览量:694,Q&A: 0
    [Abstract]

    In the Japanese rhinoceros beetle Trypoxylus dichotomus, various candidate genes required for a specific phenotype of interest are listed by next-generation sequencing analysis. Their functions were investigated using RNA interference (RNAi) method, the only gene function analysis tool for T. dichotomus developed so far. The summarized procedure

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    Isolation and Culture of Cranial Neural Crest Cells from the First Branchial Arch of Mice
    小鼠第一鳃弓颅神经嵴细胞的分离培养
    作者:Hiroki Ueharu, Jingwen Yang, Yoshihiro Komatsu and Yuji Mishina日期:04/05/2022,浏览量:442,Q&A: 0
    [Abstract]

    Craniofacial anomalies (CFA) are a diverse group of deformities, which affect the growth of the head and face. Dysregulation of cranial neural crest cell (NCC) migration, proliferation, differentiation, and/or cell fate specification have been reported to contribute to CFA. Understanding of the mechanisms through which cranial NCCs contribute

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    Generation, Maintenance and HBV Infection of Human Liver Organoids
    人肝类器官的产生、维持和HBV感染
    作者:Shahla Romal, Tanvir Hossain and Tokameh Mahmoudi日期:03/20/2022,浏览量:899,Q&A: 0
    [Abstract]

    Hepatitis B virus (HBV) infection represents a major public health problem infecting approximately 400 million people worldwide. Despite the availability of a preventive vaccine and anti-viral therapies, chronic HBV infection remains a major health issue because it increases the risk of developing liver cirrhosis and hepatocellular carcinoma

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    3D-Structured Illumination Microscopy of Centrosomes in Human Cell Lines
    人类细胞系中心体的 3D 结构照明显微镜
    作者:Kari-Anne M. Frikstad, Kay O. Schink, Sania Gilani, Lotte B. Pedersen and Sebastian Patzke日期:03/20/2022,浏览量:703,Q&A: 0
    [Abstract]

    The centrosome is the main microtubule-organizing center of animal cells, and is composed of two barrel-shaped microtubule-based centrioles embedded in protein dense pericentriolar material. Compositional and architectural re-organization of the centrosome drives its duplication, and enables its microtubule-organizing activity and capability to

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    Measurement of Contractile Ring Tension Using Two-photon Laser Ablation during Drosophila Cellularization
    果蝇细胞化过程中使用双光子激光消融测量收缩环张力
    作者:Swati Sharma and Richa Rikhy日期:03/20/2022,浏览量:373,Q&A: 0
    [Abstract]

    Cytokinesis occurs at the final step of cell division and leads to the separation of daughter cells. It requires assembly and constriction of the actomyosin contractile ring. The phases of assembly and constriction of the contractile ring show an increase in tension in the actomyosin complex. The measurement of tension in the contractile ring is

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    TUNEL Labeling to Detect Double-stranded DNA Breaks in Caenorhabditis elegans Gonads
    TUNEL 标记检测秀丽隐杆线虫性腺中的双链 DNA 断裂
    作者:Peter A. Kropp, Kyle Rhodehouse and Andy Golden日期:03/20/2022,浏览量:407,Q&A: 0
    [Abstract]

    Analysis of DNA double strand breaks (DSBs) is important for understanding dyshomeostasis within the nucleus, impaired DNA repair mechanisms, and cell death. In the C. elegans germline, DSBs are important indicators of all three above-mentioned conditions. Although multiple methods exist to assess apoptosis in the germline of C. elegans, direct

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    Labeling and Tracking Mitochondria with Photoactivation in Drosophila Embryos
    果蝇胚胎中用光活化标记和跟踪线粒体
    作者:Sayali Chowdhary and Richa Rikhy日期:03/05/2022,浏览量:1273,Q&A: 0
    [Abstract]

    Mitochondria are relatively small, fragmented, and abundant in the large embryos of Drosophila, Xenopus and zebrafish. It is essential to study their distribution and dynamics in these embryos to understand the mechanistic role of mitochondrial function in early morphogenesis events. Photoactivation of mitochondrially tagged GFP (mito-PA-GFP) is

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    NBD-lipid Uptake Assay for Mammalian Cell Lines
    哺乳动物细胞系的 NBD 脂质摄取测定
    作者:Sara Abad Herrera, Marta Grifell-Junyent and Thomas Günther Pomorski日期:02/20/2022,浏览量:1549,Q&A: 0
    [Abstract]

    All eukaryotic cells are equipped with transmembrane lipid transporters, which are key players in membrane lipid asymmetry, vesicular trafficking, and membrane fusion. The link between mutations in these transporters and disease in humans highlights their essential role in cell homeostasis. Yet, many key features of their activities, their

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