发育生物学

分类

    现刊
    Simultaneous Fluorescent Recordings of Extracellular ATP and Intracellular Calcium in Mammalian Cells
    哺乳动物细胞胞外ATP和胞内钙离子的协同荧光记录
    作者:Nicholas Mikolajewicz and Svetlana V. Komarova日期:05/20/2019,浏览量:368,Q&A: 0
    Extracellular ATP is a potent signaling molecule that stimulates intracellular calcium responses through purinergic (P2) receptors in mammalian cells. While extracellular ATP and intracellular calcium can be measured separately, simultaneous ...
    Simultaneous Fluorescent Recordings of Extracellular ATP and Intracellular Calcium in Mammalian Cells
    哺乳动物细胞胞外ATP和胞内钙离子的协同荧光记录
    作者:Nicholas Mikolajewicz and Svetlana V. Komarova日期:05/20/2019,浏览量:368,Q&A: 0
    [Abstract] Extracellular ATP is a potent signaling molecule that stimulates intracellular calcium responses through purinergic (P2) receptors in mammalian cells. While extracellular ATP and intracellular calcium can be measured separately, simultaneous monitoring can offer additional insights into P2 receptor physiology. This protocol takes advantage of the ...
    Quantification of the Humidity Effect on HR by Ion Leakage Assay
    离子渗漏实验定量分析湿度对超敏反应的影响
    作者:Musoki Mwimba and Xinnian Dong日期:04/05/2019,浏览量:402,Q&A: 0
    [Abstract] We describe a protocol to measure the contribution of humidity on cell death during the effector-triggered immunity (ETI), the plant immune response triggered by the recognition of pathogen effectors by plant resistance genes. This protocol quantifies tissue cell death by measuring ion leakage due to loss of membrane integrity during the ...
    Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
    整体原位杂交检测mRNA并提取DNA检测斑马鱼胚胎的基因分型
    作者:Rachna Narayanan and Andrew C. Oates日期:03/20/2019,浏览量:1140,Q&A: 0
    [Abstract] In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes incorporating non-radioactive labels that can be detected by immunohistochemistry and coupled to ...
    An Improved Staining Method for Low Signal LacZ Reporter Detection in Mouse Embryos
    一种改进的可用于检测小鼠胚胎弱LacZ报告信号的染色法
    作者:Xiaopeng Shen, Feng Xu, Shen Wu, Meng Li, Jingyi Zhang, Rui Liang and Yu Liu日期:03/05/2019,浏览量:560,Q&A: 0
    [Abstract] In many fields of biology, especially in the field of developmental biology, LacZ reporter staining is an approach used to monitor gene expression patterns. In the LacZ reporter system, the LacZ gene is inserted in the endogenous location of the target gene via gene knock-in or by constructing a transgenic cassette in ...
    High-resolution Immunoelectron Microscopy Techniques for Revealing Distinct Subcellular Type 1 Cannabinoid Receptor Domains in Brain
    检测脑中不同亚细胞1型大麻素受体结构域的高分辨率免疫电镜技术
    作者:Nagore Puente, Itziar Bonilla-Del Río, Svein Achicallende, Patrick C. Nahirney and Pedro Grandes日期:01/20/2019,浏览量:899,Q&A: 0
    [Abstract] Activation of type 1 cannabinoid (CB1) receptors by endogenous, exogenous (cannabis derivatives) or synthetic cannabinoids (i.e., CP 55.940, Win-2) has a wide variety of behavioral effects due to the presence of CB1 receptors in the brain. In situ hybridization and immunohistochemical techniques have been ...
    Heterochronic Phenotype Analysis of Hypodermal Seam Cells in Caenorhabditis elegans
    秀丽隐杆线虫下皮干细胞的异时表型分析
    作者:Yon Ju Ji and Jiou Wang日期:01/05/2019,浏览量:954,Q&A: 0
    [Abstract] Heterochrony refers to changes in the timing of developmental events, and it is precisely regulated in the organisms by the heterochronic genes such as C. elegans lin-4 and let-7. Mutations in these genes cause precocious or retarded development of certain cell lineages. With well-defined cell lineages, C. elegans ...
    Quantification of Mouse Hematopoietic Progenitors’ Formation Using Time-lapse Microscopy and Image Analysis
    使用延时显微技术和图像分析定量小鼠造血祖细胞的形成
    作者:Isabelle Bergiers, Christian Tischer, Özge Vargel Bölükbaşı and Christophe Lancrin日期:01/05/2019,浏览量:1156,Q&A: 0
    [Abstract] In vitro differentiation of mouse embryonic stem cells (mESCs) towards blood cells constitutes a well-established system to study the endothelial-to-hematopoietic transition (EHT) at the onset of blood development. Assessing the emergence of small non-adherent round blood cells in the culture without disturbing it is essential to evaluate ...
    Cartilage Induction from Mouse Mesenchymal Stem Cells in High-density Micromass Culture
    高密度微团培养的小鼠骨髓间充质干细胞诱导分化生成的软骨
    作者:Takashi Iezaki, Kazuya Fukasawa, Takanori Yamada, Manami Hiraiwa, Katsuyuki Kaneda and Eiichi Hinoi日期:01/05/2019,浏览量:796,Q&A: 0
    [Abstract] Mesenchymal stem cells have the ability to differentiate into multiple lineages, including adipocytes, osteoblasts and chondrocytes. Mesenchymal stem cells can be induced to differentiate into chondrocytes in extracellular matrices, such as alginate or collagen gel. Mesenchymal stem cells in a cell pellet or micromass culture can be also induced ...
    Notochord Injury Assays that Stimulate Transcriptional Responses in Zebrafish Larvae
    诱导幼年期斑马鱼转录响应的脊索损伤实验
    [Abstract] Zebrafish have become an increasingly important model organism in the field of wound healing and regenerative medicine, due to their high regenerative capacity coupled with high-resolution imaging in living animals. In a recent study, we described multiple physical and chemical methods to induce notochord injury that led to highly specific ...
    A Functionally Robust Phenotypic Screen that Identifies Drug Resistance-associated Genes Using 3D Cell Culture
    利用3D细胞培养鉴定耐药相关基因的一种功能性稳健表型筛选
    作者:Sun-Young Lee and Mina J. Bissell日期:11/20/2018,浏览量:1375,Q&A: 0
    [Abstract] Drug resistance is a major obstacle in cancer treatment: A case in point is the failure of cancer patients to respond to tyrosine kinase inhibitors (TKI) of EGFR, a receptor that is highly expressed in many cancers. Identification of the targets and delineation of mechanisms of drug resistance remain major challenges. Traditional pharmacological ...