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发育生物学

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    现刊
    Ex vivo Trophoblast-specific Genetic Manipulation Using Lentiviral Delivery
    使用慢病毒递送的离体滋养层特定基因操作
    作者:Damayanti Chakraborty, Masanaga Muto and Michael J. Soares日期:12/20/2017,浏览量:257,Q&A: 0
    [Abstract] In this protocol report, we describe a lentiviral gene delivery technique for genetic modification of the rat trophoblast cell lineage. Lentiviral packaged gene constructs can be efficiently and specifically delivered to the trophoblast cell lineage of the blastocyst. The consequences of ‘gain-of-function’ and ‘loss-of-function’ blastocyst ...
    Biochemical Isolation of Myonuclei from Mouse Skeletal Muscle Tissue
    从小鼠骨骼肌组织中骨骼肌细胞核的生化分离
    作者:Alicia A Cutler, Anita H Corbett and Grace K Pavlath日期:12/20/2017,浏览量:387,Q&A: 0
    [Abstract] Skeletal muscle provides the contractile force necessary for movement, swallowing, and breathing and, consequently, is necessary for survival. Skeletal muscle cells are unique in that they are extremely large cells containing thousands of nuclei. These nuclei must all work in concert to maintain skeletal muscle function and thereby maintain life. ...
    Notch Ligand Binding Assay Using Flow Cytometry
    使用流式细胞术的Notch配体结合检测
    作者:Shweta Varshney and Pamela Stanley日期:12/05/2017,浏览量:515,Q&A: 0
    [Abstract] Notch signaling is an evolutionarily conserved signaling pathway that plays an indispensable role during development, and in the maintenance of homeostatic processes, in a wide variety of tissues (Kopan, 2012; Hori et al., 2013). The multifaceted roles of Notch signaling are stringently regulated at different levels. One of the most ...
    Mandibular Explant Assay for Investigating Extrinsic Stimuli on Bone and Cartilage Development
    研究骨及软骨外源刺激的下颌外植体实验
    作者:Jamie Jiang, Jessica W. Bertol and Walid D. Fakhouri日期:12/05/2017,浏览量:367,Q&A: 0
    [Abstract] A major issue in developmental biology is to determine how embryonic tissues respond to molecular signals in a timely manner and given the position-restricted instructions during morphogenesis, of which Meckel’s cartilage is a classical example. The ex-vivo explant model is a practical and convenient system that allows investigation of ...
    Rapid IFM Dissection for Visualizing Fluorescently Tagged Sarcomeric Proteins
    快速解剖间接飞行肌用于荧光标记肌节蛋白的观察
    作者:Yu Shu Xiao, Frieder Schöck and Nicanor González-Morales日期:11/20/2017,浏览量:516,Q&A: 0
    [Abstract] Sarcomeres, the smallest contractile unit of muscles, are arguably the most impressive actomyosin structure. Yet a complete understanding of sarcomere formation and maintenance is missing. The Drosophila indirect flight muscle (IFM) has proven to be a very valuable model to study sarcomeres. Here, we present a protocol for the rapid ...
    Protocol for Notch-ligand Binding Assays Using Dynabeads
    使用免疫磁珠进行Notch配体结合分析的实验方案
    作者:Shogo Sawaguchi, Mitsutaka Ogawa and Tetsuya Okajima日期:10/20/2017,浏览量:933,Q&A: 0
    [Abstract] This protocol describes how to measure interaction between Notch receptors and their ligands by cell-based assay using Dynabeads. We have used the protocol to determine binding capacity between Notch1-transfected HEK293T cells and ligand-coated Dynabeads. Expression of Eogt in Notch1-expressing cells promoted binding toward DLL4-coated ...
    In vitro Co-culture of Mesenchymal Stem Cells and Endothelial Colony Forming Cells
    间充质干细胞和内皮集落形成细胞的体外共培养
    作者:Abbas Shafiee and Kiarash Khosrotehrani日期:10/20/2017,浏览量:1090,Q&A: 0
    [Abstract] The discovery of endothelial colony forming cells (ECFCs) with robust self-renewal and de novo vessel formation potentials suggests that ECFCs can be an excellent cell source for cardiovascular diseases treatment through improving neovascularization in the ischemic tissues. However, their engraftment after transplantation resulted to be low. ...
    Labelling HaloTag Fusion Proteins with HaloTag Ligand in Living Cells
    在活细胞中使用HaloTag配体标记HaloTag融合蛋白
    作者:Huy Nguyen Duc and Xiaojun Ren 日期:09/05/2017,浏览量:999,Q&A: 0
    [Abstract] HaloTag has been widely used to label proteins in vitro and in vivo (Los et al., 2008). In this protocol, we describe labelling HaloTag-Cbx fusion proteins by HaloTag ligands for live-cell single-molecule imaging (Zhen et al., 2016).
    Isolation of Mouse Cardiac Neural Crest Cells and Their Differentiation into Smooth Muscle Cells
    小鼠心脏神经嵴细胞的分离及向平滑肌细胞的分化
    作者:Xia Wang and Sophie Astrof日期:09/05/2017,浏览量:934,Q&A: 0
    [Abstract] Cardiac neural crest cells (CNCCs) originate at the dorsal edge of the neural tube between the otic pit and the caudal edge of the 3rd somite, and migrate into the pharyngeal arches and the heart. We have shown that fibronectin (Fn1) plays an important role in the development of the CNCC by regulating the differentiation of CNCCs into ...
    Ex vivo Ooplasmic Extract from Developing Drosophila Oocytes for Quantitative TIRF Microscopy Analysis
    从在发育的果蝇卵母细胞中离体提取卵磷脂用于定量TIRF显微镜分析
    作者:Imre Gáspár and Anne Ephrussi日期:07/05/2017,浏览量:1445,Q&A: 0
    [Abstract] Understanding the dynamic behavior and the continuously changing composition of macromolecular complexes, subcellular structures and organelles is one of areas of active research in both cell and developmental biology, as these changes directly relate to function and subsequently to the development and homeostasis of the organism. Here, we ...