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    现刊
    MicroScale Thermophoresis as a Tool to Study Protein-peptide Interactions in the Context of Large Eukaryotic Protein Complexes
    微量热泳动法研究大真核生物蛋白复合物中蛋白质 - 肽相互作用
    作者:Maximilian G. Plach, Klaus Grasser and Thomas Schubert日期:12/05/2017,浏览量:564,Q&A: 0
    [Abstract] Protein-peptide interactions are part of many physiological processes, for example, epigenetics where peptide regions of histone complexes are crucial for regulation of chromatin structure. Short peptides are often also used as alternatives to small molecule drugs to target protein complexes. Studying the interactions between proteins and peptides ...
    Drosophila Model of Leishmania amazonensis Infection
    亚马逊利什曼原虫感染的果蝇模型
    作者:Kendi Okuda and Neal Silverman日期:12/05/2017,浏览量:372,Q&A: 0
    [Abstract] This protocol describes how to generate and harvest antibody-free L. amazonensis amastigotes, and how to infect adult Drosophila melanogaster with these parasites. This model recapitulates key aspects of the interactions between Leishmania amastigotes and animal phagocytes.
    Quantification of Trypanosoma cruzi in Tissue and Trypanosoma cruzi Killing Assay
    组织中克氏锥虫的定量及克氏锥虫杀灭试验
    作者:Hisako Kayama, Shoko Kitada and Kiyoshi Takeda日期:11/20/2017,浏览量:508,Q&A: 0
    [Abstract] Infection with Trypanosoma cruzi causes Chagas disease. The methods provided here allow for the quantification of T. cruzi in the liver, heart, and blood of intraperitoneally-infected mice and analysis of the killing activity of the cells infected with T. cruzi in vitro.
    Liposome Disruption Assay to Examine Lytic Properties of Biomolecules
    采用脂质体破碎测定法检测生物分子的裂解性质
    作者:John R. Jimah, Paul H. Schlesinger and Niraj H. Tolia日期:08/05/2017,浏览量:1549,Q&A: 0
    [Abstract] Proteins may have three dimensional structural or amino acid features that suggest a role in targeting and disrupting lipids within cell membranes. It is often necessary to experimentally investigate if these proteins and biomolecules are able to disrupt membranes in order to conclusively characterize the function of these biomolecules. Here, we ...
    Plasmodium Sporozoite Motility on Flat Substrates
    疟原虫孢子体在平面基质上的能动性
    作者:Henriette L Prinz, Julia M Sattler and Friedrich Frischknecht日期:07/20/2017,浏览量:1029,Q&A: 0
    [Abstract] Plasmodium sporozoites are the infectious, highly motile forms of the malaria parasite transmitted by Anopheles mosquitoes. Sporozoite motility can be assessed following the dissection of Anopheles salivary glands and isolation of sporozoites in vitro.
    Endogenous C-terminal Tagging by CRISPR/Cas9 in Trypanosoma cruzi
    在克氏锥虫中利用CRISPR/Cas9内源标记靶基因C端
    作者:Noelia Lander, Miguel A. Chiurillo, Aníbal E. Vercesi and Roberto Docampo日期:05/20/2017,浏览量:2577,Q&A: 0
    [Abstract] To achieve the C-terminal tagging of endogenous proteins in T. cruzi we use the Cas9/pTREX-n vector (Lander et al., 2015) to insert a specific tag sequence (3xHA or 3xc-Myc) at the 3’ end of a specific gene of interest (GOI). Chimeric sgRNA targeting the 3’ end of the GOI is PCR-amplified and cloned into Cas9/pTREX-n vector. Then ...
    Isolation of Latex Bead Phagosomes from Dictyostelium for in vitro Functional Assays
    分离盘基网柄菌的乳胶珠吞噬体用于体外功能分析
    作者:Ashwin D’Souza, Paulomi Sanghavi, Ashim Rai, Divya Pathak and Roop Mallik日期:12/05/2016,浏览量:2558,Q&A: 0
    [Abstract] We describe a protocol to purify latex bead phagosomes (LBPs) from Dictyostelium cells. These can be later used for various in vitro functional assays. For instance, we use these LBPs to understand the microtubule motor-driven transport on in vitro polymerized microtubules. Phagosomes are allowed to mature for defined ...
    In vitro Fluorescent Matrix Degradation Assay for Entamoeba histolytica
    痢疾变形虫的体外荧光基质降解试验
    作者:Merlyn Emmanuel and Sunando Datta日期:03/20/2016,浏览量:2581,Q&A: 0
    [Abstract] Fluorescent matrix degradation assay is a popular and widely used assay in the field of invadopodium biology (Artym et al., 2009). Matrix remodeling and degradation can be observed under both physiological and pathological conditions. Cancer cells extensively remodel and degrade the underlying matrix by employing actin-rich protrusive ...
    Dictyostelium Cultivation, Transfection, Microscopy and Fractionation
    网炳菌属的培养、转染、显微镜检查和分级分离
    作者:Jennifer Hirst, Robert R Kay and David Traynor日期:06/05/2015,浏览量:5204,Q&A: 0
    [Abstract] The real time visualisation of fluorescently tagged proteins in live cells using ever more sophisticated microscopes has greatly increased our understanding of the dynamics of key proteins during fundamental physiological processes such as cell locomotion, chemotaxis, cell division and membrane trafficking. In addition the fractionation of cells ...
    Cyst Detection in Toxoplasma gondii Infected Mice and Rats Brain
    刚地弓形虫感染小鼠和大鼠脑部囊肿检测
    作者:Valeria Bellini, Corinne Loeuillet, Céline Massera, Marie-France Cesbron-Delauw and Pierre Cavaillès日期:04/05/2015,浏览量:3802,Q&A: 0
    [Abstract] Toxoplasmosis caused by the intracellular parasite Toxoplasma gondii, is characterized by a life-long chronic infection. The parasite is an efficient neurotropic infectious agent that establishes its “safe” life by forming intracellular cysts in chronically infected animals and humans. This protocol describes the specific recipes and ...