系统生物学

分类

    现刊
    A Molecular Cloning and Sanger Sequencing-based Protocol for Detecting Site-specific DNA Methylation
    一种基于分子克隆和Sanger测序的位点特异性DNA甲基化检测方案
    作者:Wei Guo, Anthony Cannon and Damon Lisch日期:05/05/2022,浏览量:427,Q&A: 0

    DNA methylation is a conserved chemical modification, by which methyl groups are added to the cytosine of DNA molecules. Methylation can influence gene expression without changing the sequence of a particular gene. This epigenetic effect is an

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    A Molecular Cloning and Sanger Sequencing-based Protocol for Detecting Site-specific DNA Methylation
    一种基于分子克隆和Sanger测序的位点特异性DNA甲基化检测方案
    作者:Wei Guo, Anthony Cannon and Damon Lisch日期:05/05/2022,浏览量:427,Q&A: 0
    [Abstract]

    DNA methylation is a conserved chemical modification, by which methyl groups are added to the cytosine of DNA molecules. Methylation can influence gene expression without changing the sequence of a particular gene. This epigenetic effect is an intriguing phenomenon that has puzzled biologists for years. By probing the temporal and spatial patterns

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    circFL-seq, A Full-length circRNA Sequencing Method
    circFL-seq,一种全长circRNA的测序方法
    作者:Zelin Liu and Ence Yang日期:04/20/2022,浏览量:756,Q&A: 1
    [Abstract]

    Due to overlapping sequences with linear cognates, identifying internal sequences of circular RNA (circRNA) remains a challenge. Recently, we have developed a full-length circRNA sequencing method (circFL-seq) and computational pipeline, to profile ordinary and fusion circRNA at the isoform level. Compared to short-read RNA-seq, rolling circular

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    Whole-genome Methylation Analysis of APOBEC Enzyme-converted DNA (~5 kb) by Nanopore Sequencing
    通过纳米孔测序对 APOBEC 酶转化 DNA (~5 kb) 进行全基因组甲基化分析
    作者:Suzuko Zaha, Yoshitaka Sakamoto, Satoi Nagasawa, Sumio Sugano, Ayako Suzuki, Yutaka Suzuki and Masahide Seki日期:03/05/2022,浏览量:1678,Q&A: 0
    [Abstract]

    In recent years, DNA methylation research has been accelerated by the advent of nanopore sequencers. However, read length has been limited by the constraints of base conversion using the bisulfite method, making analysis of chromatin content difficult. The read length of the previous method combining bisulfite conversion and long-read sequencing

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    Enrichment of Tyrosine Phosphorylated Peptides for Quantitative Mass Spectrometry Analysis of RTK Signaling Dynamics
    丰富酪氨酸磷酸化肽用于RTK信号动力学的定量质谱分析
    作者:Vidyasiri Vemulapalli, Stephen C. Blacklow, Steven P. Gygi and Alison Erickson日期:02/05/2022,浏览量:1089,Q&A: 0
    [Abstract]

    Cells sense and respond to mitogens by activating a cascade of signaling events, primarily mediated by tyrosine phosphorylation (pY). Because of its key roles in cellular homeostasis, deregulation of this signaling is often linked to oncogenesis. To understand the mechanisms underlying these signaling pathway aberrations, it is necessary to

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    Subcellular RNA-seq for the Analysis of the Dendritic and Somatic Transcriptomes of Single Neurons
    用于分析单个神经元树突和体细胞转录组的亚细胞 RNA-seq
    作者:Julio D. Perez and Erin M. Schuman日期:01/05/2022,浏览量:1338,Q&A: 0
    [Abstract]

    In neurons, local translation in dendritic and axonal compartments allows for the fast and on-demand modification of the local proteome. As the last few years have witnessed dramatic advancements in our appreciation of the brain’s neuronal diversity, it is increasingly relevant to understand how local translation is regulated according to

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    作者:海波 单 and Jianguo Ruan日期:12/20/2021,浏览量:643,Q&A: 19
    [Abstract]

    The α-β tubulin heterodimer undergoes subtle conformational changes during microtubule assembly. These can be modulated by external factors, whose effects on microtubule structure can be characterized on 2D views obtained by cryo-electron microscopy. Analysis of microtubule images is facilitated if they are straight enough to interpret

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    TGIRT-seq Protocol for the Comprehensive Profiling of Coding and Non-coding RNA Biotypes in Cellular, Extracellular Vesicle, and Plasma RNAs
    用于全面分析细胞、细胞外囊泡和血浆 RNA 中编码和非编码 RNA 生物型的 TGIRT-seq 方法
    作者:Hengyi Xu, Ryan M. Nottingham and Alan M. Lambowitz日期:12/05/2021,浏览量:1160,Q&A: 0
    [Abstract]

    High-throughput RNA sequencing (RNA-seq) has extraordinarily advanced our understanding of gene expression and disease etiology, and is a powerful tool for the identification of biomarkers in a wide range of organisms. However, most RNA-seq methods rely on retroviral reverse transcriptases (RTs), enzymes that have inherently low fidelity and

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    Macroscopic Structural and Connectome Mapping of the Mouse Brain Using Diffusion Magnetic Resonance Imaging
    利用弥散磁共振成像绘制小鼠大脑的宏观结构和连接组图
    作者:Tanzil Mahmud Arefin, Choong Heon Lee, Jordon D. White, Jiangyang Zhang and Arie Kaffman日期:11/20/2021,浏览量:1683,Q&A: 0
    [Abstract]

    Translational work in rodents elucidates basic mechanisms that drive complex behaviors relevant to psychiatric and neurological conditions. Nonetheless, numerous promising studies in rodents later fail in clinical trials, highlighting the need for improving the translational utility of preclinical studies in rodents. Imaging of small rodents

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    Histone modification ChIP-seq on Arabidopsis thaliana Plantlets
    拟南芥植株上的组蛋白修饰ChIP-seq
    作者:Juan S. Ramirez-Prado, David Latrasse and Moussa Benhamed日期:11/05/2021,浏览量:1643,Q&A: 1
    [Abstract]

    Characterizing the molecular mechanisms regulating gene expression is crucial for understanding the regulatory processes underlying physiological responses to environmental and developmental signals in eukaryotes. The covalent modification of histones contributes to the compaction levels of chromatin, as well as the recruitment of the

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    Protocols to Induce, Prevent, and Treat Post-traumatic Stress Disorder-like Memory in Mice: Optogenetics and Behavioral Approaches
    诱导、预防和治疗小鼠创伤后应激障碍样记忆的方案:光遗传学和行为方法
    作者:Aline S. Al Abed, Azza Sellami, Eva-Gunnel Ducourneau, Chloé Bouarab, Aline Marighetto and Aline Desmedt日期:10/05/2021,浏览量:1338,Q&A: 0
    [Abstract]

    One of the cardinal features of post-traumatic stress disorder (PTSD) is a paradoxical memory alteration including both emotional hypermnesia for salient trauma-related cues and amnesia for the surrounding traumatic context. Interestingly, some clinical studies have suggested that contextual amnesia would causally contribute to the PTSD-related

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