哺乳纲

分类

    现刊
    DNA Immunization Using in vivo Electroporation for Generating Monoclonal Antibodies Against Mouse IL-9R
    利用体内电穿孔产生小鼠IL-9R单克隆抗体进行基因免疫
    作者:Shogo Takatsuka, Hiroyuki Yamada, Kei Haniuda, Marina Ichihashi, Joe Chiba and Daisuke Kitamura日期:02/20/2019,浏览量:54,Q&A: 0
    Membrane proteins such as cytokine receptors and G protein-coupled receptors can be drug targets. Recently, we have generated specific monoclonal antibodies (mAbs) against the mouse IL-9 receptor (IL-9R) and found that IL-9R on memory B cells have ...
    Induced Germinal Center B Cell Culture System
    诱导生发中心B细胞培养系统
    作者:Kei Haniuda and Daisuke Kitamura日期:02/20/2019,浏览量:99,Q&A: 0
    The germinal center (GC) is the site where B cells undergo clonal expansion, affinity-based selection, and differentiation into memory B cells or plasma cells. It has been difficult to elucidate regulatory mechanisms for the dynamic GC B cell ...
    Determination of Cellular Uptake and Endocytic Pathways
    细胞摄取和内吞途径的检测
    作者:Jing Gong, Hong-Xia Wang and Kam W. Leong日期:02/20/2019,浏览量:98,Q&A: 0
    Efficiency of drug and gene delivery via nonviral vehicles is contingent on proper cellular uptake and intracellular release. Further, various cargos, such as nucleases for gene editing or inhibitors for endosomal receptors, require transport to ...
    Assessing Olfaction Using Ultrasonic Vocalization Recordings in Mouse Pups with a Sono-olfactometer
    使用超声波嗅觉计通过超声波发生记录评估小鼠幼崽的嗅觉
    作者:Sébastien Wagner, Pierre-Marie Lledo and Françoise Lazarini日期:02/20/2019,浏览量:74,Q&A: 0
    Olfaction is the first sensory modality to develop during fetal life in mammals, and plays a key role in the various behaviors of neonates such as feeding and social interaction. Odorant cues (i.e., mother or predator scents) can trigger ...
    On-demand Labeling of SNAP-tagged Viral Protein for Pulse-Chase Imaging, Quench-Pulse-Chase Imaging, and Nanoscopy-based Inspection of Cell Lysates
    用于脉冲追踪成像,猝发脉冲追踪成像和基于纳米显微技术的细胞裂解物检测的融合SNAP病毒蛋白的按需标记
    作者:Roland Remenyi, Raymond Li and Mark Harris日期:02/20/2019,浏览量:52,Q&A: 0
    Advanced labeling technologies allow researchers to study protein turnover inside intact cells and to track the labeled protein in downstream applications. In the context of a viral infection, the combination of imaging and fluorescent labeling of ...
    Isolation of Neural Stem Cells from the Embryonic Mouse Hippocampus for in vitro Growth or Engraftment into a Host Tissue
    从胚胎小鼠海马分离神经干细胞用于体外生长或植入宿主组织
    作者:Oksana Rybachuk, Olga Kopach, Tetyana Pivneva and Vitaliy Kyryk日期:02/20/2019,浏览量:71,Q&A: 0
    For both stem cell research and treatment of the central nervous system disorders, neural stem/progenitor cells (NSPCs) represent an important breakthrough tool. In the expanded stem cell-based therapy use, NSPCs not only provide a powerful cell ...
    Cell Microencapsulation and Cryopreservation with Low Molecular Weight Hyaluronan and Dimethyl Sulfoxide
    利用低分子量透明质酸和二甲基亚砜进行细胞微胶囊化和冷冻保存
    作者:H. Gurruchaga, L. Saenz del Burgo, G. Orive, R. M. Hernandez, J. Ciriza and J. L. Pedraz日期:02/20/2019,浏览量:81,Q&A: 0
    Cryopreservation is commonly used for the storage of cells, tissues, organs or 3D cell-based products using ultra-low temperatures, which involves the immersion in liquid nitrogen for their long-term preservation. The cryopreservation of several ...
    Isolation of Multipotent Mesenchymal Stem Cells from Human Extraocular Muscle Tissue
    人眼外肌组织多能间充质干细胞的分离
    作者:Amit Sharma, Darilang Mawrie, Damarais Magdalene and Bithiah Grace Jaganathan日期:02/20/2019,浏览量:75,Q&A: 0
    Mesenchymal stem cells (MSCs) have attracted significant attention as potential therapeutic cells to treat various diseases ranging from tissue injuries, graft versus host disease, degenerative diseases and cancer. Since the initial discovery of ...
    DNA Immunization Using in vivo Electroporation for Generating Monoclonal Antibodies Against Mouse IL-9R
    利用体内电穿孔产生小鼠IL-9R单克隆抗体进行基因免疫
    作者:Shogo Takatsuka, Hiroyuki Yamada, Kei Haniuda, Marina Ichihashi, Joe Chiba and Daisuke Kitamura日期:02/20/2019,浏览量:54,Q&A: 0
    [Abstract] Membrane proteins such as cytokine receptors and G protein-coupled receptors can be drug targets. Recently, we have generated specific monoclonal antibodies (mAbs) against the mouse IL-9 receptor (IL-9R) and found that IL-9R on memory B cells have critical roles in T-dependent immune response. So far, most antibodies against cell surface proteins ...
    Induced Germinal Center B Cell Culture System
    诱导生发中心B细胞培养系统
    作者:Kei Haniuda and Daisuke Kitamura日期:02/20/2019,浏览量:99,Q&A: 0
    [Abstract] The germinal center (GC) is the site where B cells undergo clonal expansion, affinity-based selection, and differentiation into memory B cells or plasma cells. It has been difficult to elucidate regulatory mechanisms for the dynamic GC B cell maturation and differentiation, partly because experimental manipulation of GC B cells in vivo ...
    Determination of Cellular Uptake and Endocytic Pathways
    细胞摄取和内吞途径的检测
    作者:Jing Gong, Hong-Xia Wang and Kam W. Leong日期:02/20/2019,浏览量:98,Q&A: 0
    [Abstract] Efficiency of drug and gene delivery via nonviral vehicles is contingent on proper cellular uptake and intracellular release. Further, various cargos, such as nucleases for gene editing or inhibitors for endosomal receptors, require transport to specific compartments of the cell. Hence, characterization of cellular uptake and endocytic pathways is ...
    Assessing Olfaction Using Ultrasonic Vocalization Recordings in Mouse Pups with a Sono-olfactometer
    使用超声波嗅觉计通过超声波发生记录评估小鼠幼崽的嗅觉
    作者:Sébastien Wagner, Pierre-Marie Lledo and Françoise Lazarini日期:02/20/2019,浏览量:74,Q&A: 0
    [Abstract] Olfaction is the first sensory modality to develop during fetal life in mammals, and plays a key role in the various behaviors of neonates such as feeding and social interaction. Odorant cues (i.e., mother or predator scents) can trigger potentiation or inhibition of ultrasonic vocalizations (USV) emitted by pups following their ...
    On-demand Labeling of SNAP-tagged Viral Protein for Pulse-Chase Imaging, Quench-Pulse-Chase Imaging, and Nanoscopy-based Inspection of Cell Lysates
    用于脉冲追踪成像,猝发脉冲追踪成像和基于纳米显微技术的细胞裂解物检测的融合SNAP病毒蛋白的按需标记
    作者:Roland Remenyi, Raymond Li and Mark Harris日期:02/20/2019,浏览量:52,Q&A: 0
    [Abstract] Advanced labeling technologies allow researchers to study protein turnover inside intact cells and to track the labeled protein in downstream applications. In the context of a viral infection, the combination of imaging and fluorescent labeling of viral proteins sheds light on their biological activity and interaction with the host cell. Initial ...
    Isolation of Neural Stem Cells from the Embryonic Mouse Hippocampus for in vitro Growth or Engraftment into a Host Tissue
    从胚胎小鼠海马分离神经干细胞用于体外生长或植入宿主组织
    作者:Oksana Rybachuk, Olga Kopach, Tetyana Pivneva and Vitaliy Kyryk日期:02/20/2019,浏览量:71,Q&A: 0
    [Abstract] For both stem cell research and treatment of the central nervous system disorders, neural stem/progenitor cells (NSPCs) represent an important breakthrough tool. In the expanded stem cell-based therapy use, NSPCs not only provide a powerful cell source for neural cell replacement but a useful model for developmental biology research. Despite ...
    Cell Microencapsulation and Cryopreservation with Low Molecular Weight Hyaluronan and Dimethyl Sulfoxide
    利用低分子量透明质酸和二甲基亚砜进行细胞微胶囊化和冷冻保存
    作者:H. Gurruchaga, L. Saenz del Burgo, G. Orive, R. M. Hernandez, J. Ciriza and J. L. Pedraz日期:02/20/2019,浏览量:81,Q&A: 0
    [Abstract] Cryopreservation is commonly used for the storage of cells, tissues, organs or 3D cell-based products using ultra-low temperatures, which involves the immersion in liquid nitrogen for their long-term preservation. The cryopreservation of several microencapsulated cells is usually performed by the slow freezing with the dimethyl sulfoxide (DMSO) as ...
    Isolation of Multipotent Mesenchymal Stem Cells from Human Extraocular Muscle Tissue
    人眼外肌组织多能间充质干细胞的分离
    作者:Amit Sharma, Darilang Mawrie, Damarais Magdalene and Bithiah Grace Jaganathan日期:02/20/2019,浏览量:75,Q&A: 0
    [Abstract] Mesenchymal stem cells (MSCs) have attracted significant attention as potential therapeutic cells to treat various diseases ranging from tissue injuries, graft versus host disease, degenerative diseases and cancer. Since the initial discovery of MSCs in the bone marrow cells, MSCs have been successfully isolated from various adult and neo-natal ...
    Induction and Analysis of Anti-CD40-induced Colitis in Mice
    小鼠中抗-CD40抗体诱导的结肠炎的诱导和分析
    作者:Barbara Joyce-Shaikh, Daniel J. Cua and David Bauché日期:02/05/2019,浏览量:330,Q&A: 0
    [Abstract] Colon inflammation or colitis affects more than 1 million people worldwide. Several pre-clinical models, including chemical-induced (i.e., DSS, TNBS) or pathogen-induced (i.e., Citrobacter rodentium) have been used to study mechanisms involved in the development and regulation of colitis. Anti-CD40 induced colitis model ...
    Calvarial Bone Implantation and in vivo Imaging of Tumor Cells in Mice
    小鼠颅骨回植和肿瘤细胞的活体成像术
    作者:Kyoko Hashimoto, Shingo Sato, Hiroki Ochi, Shu Takeda and Mitsuru Futakuchi日期:02/05/2019,浏览量:225,Q&A: 1
    [Abstract] Bone is one of common metastasis sites for many types of cancer. In bone metastatic microenvironment, tumor-bone interactions play a significant role in the regulation of osteolytic or osteoblastic bone metastasis. In order to investigate the direct interaction between tumor cells and bone tissue, it is essential to generate appropriate animal ...