哺乳纲

分类

    现刊
    Isolation of Nuclei from Mouse Dorsal Root Ganglia for Single-nucleus Genomics
    小鼠背根神经节细胞核的分离及其单核基因组学研究
    作者:Lite Yang, Ivan Tochitsky, Clifford J. Woolf and William Renthal日期:08/05/2021,浏览量:24,Q&A: 0

    Primary somatosensory neurons, whose cell bodies reside in the dorsal root ganglion (DRG) and trigeminal ganglion, are specialized to transmit sensory information from the periphery to the central nervous system. Our molecular understanding of

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    Optical Clearing and 3D Analysis Optimized for Mouse and Human Pancreata
    小鼠和人胰腺的光学清除和三维分析优化

    The pancreas is a heavily innervated organ, but pancreatic innervation can be challenging to comprehensively assess using conventional histological methods. However, recent advances in whole-mount tissue clearing and 3D rendering techniques have

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    Isolation and in vitro Culture of Mouse Oocytes
    小鼠卵母细胞的分离与体外培养
    作者:Jessica Greaney, Goutham Narayanan Subramanian, Yunan Ye and Hayden Homer日期:08/05/2021,浏览量:23,Q&A: 0

    Females are endowed at birth with a fixed reserve of oocytes, which declines both in quantity and quality with advancing age. Understanding the molecular mechanisms regulating oocyte quality is crucial for improving the chances of pregnancy success

    ...
    Efficient and Rapid Analysis of Polysomes and Ribosomal Subunits in Cells and Tissues Using Ribo Mega-SEC
    利用Ribo Mega-SEC高效快速分析细胞和组织中的多聚体和核糖体亚基
    作者:Harunori Yoshikawa, Ramasubramanian Sundaramoorthy, Daniel Mariyappa, Hao Jiang and Angus I. Lamond日期:08/05/2021,浏览量:20,Q&A: 0

    Polysome profile analysis is a popular method for separating polysomes and ribosomal subunits and is typically achieved using a sucrose density gradient (SDG). This has remained the gold standard method since ribosomes were first discovered;

    ...
    Mouse Periosteal Cell Culture, in vitro Differentiation, and in vivo Transplantation in Tibial Fractures
    小鼠骨膜细胞培养、体外分化及胫骨骨折的体内移植
    作者:Simon Perrin, Anais Julien, Oriane Duchamp de Lageneste, Rana Abou-Khalil and Céline Colnot日期:08/05/2021,浏览量:44,Q&A: 0

    The periosteum covering the outer surface of bone contains skeletal stem/progenitor cells that can efficiently form cartilage and bone during bone repair. Several methods have been described to isolate periosteal cells based on bone scraping and/or

    ...
    A Simple Spatial-independent Associative and Reversal Learning Task in Mice
    小鼠的一个简单的空间独立的联想和逆转学习任务
    作者:Fulvio Magara, Benjamin Boury-Jamot and Hanna Hörnberg日期:08/05/2021,浏览量:17,Q&A: 0

    The ability to adapt one's behavior in response to changing circumstances, or cognitive flexibility, is often altered in neuropsychiatric and neurodevelopmental conditions. In rodents, cognitive flexibility is frequently assessed using associative

    ...
    Cell-attached and Whole-cell Patch-clamp Recordings of Dopamine Neurons in the Substantia Nigra Pars Compacta of Mouse Brain Slices
    小鼠脑片黑质致密部多巴胺神经元的细胞贴附和全细胞膜片钳记录
    作者:Stefano Cattaneo, Maria Regoni, Jenny Sassone and Stefano Taverna日期:08/05/2021,浏览量:17,Q&A: 0

    The Substantia Nigra pars compacta (SNc) is a midbrain dopaminergic nucleus that plays a key role in modulating motor and cognitive functions. It is crucially involved in several disorders, particularly Parkinson’s disease, which is

    ...
    Purification of Mitochondrial Ribosomes with the Translocase Oxa1L from HEK Cells
    用转位酶Oxa1L纯化HEK细胞线粒体核糖体
    作者:Hanting Yang and Nirupa Desai日期:08/05/2021,浏览量:19,Q&A: 0

    Mitochondrial ribosomes (mitoribosomes) perform protein synthesis inside mitochondria, the organelles responsible for energy conversion and adenosine triphosphate (ATP) production in eukaryotic cells. To investigate their functions and structures,

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    Purification and Cryo-electron Microscopy Analysis of Plant Mitochondrial Ribosomes
    植物线粒体核糖体的纯化和低温电镜分析
    作者:Florent Waltz, Philippe Giegé and Yaser Hashem日期:08/05/2021,浏览量:17,Q&A: 0

    Plants make up by far the largest part of biomass on Earth. They are the primary source of food and the basis of most drugs used for medicinal purposes. Similarly to all eukaryotes, plant cells also use mitochondria for energy production. Among

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    Purification of Recombinant Wild Type and Mutant Ryanodine Receptors Expressed in HEK293 Cells
    HEK293细胞表达的重组野生型和突变型Ryanodine受体的纯化
    作者:Yifan Hu, Kavita A. Iyer, Ashok R. Nayak, Nagomi Kurebayashi, Takashi Murayama and Montserrat Samsó日期:08/05/2021,浏览量:21,Q&A: 0

    High quantities of purified ryanodine receptor (RyR), a large (2.26 MDa) intracellular homotetrameric membrane protein, can be obtained from heterologous expression in HEK293 cells and used for structure determination by cryo-EM. The advantage of

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    Fe-NTA Microcolumn Purification of Phosphopeptides from Immunoprecipitation (IP) Eluates for Mass Spectrometry Analysis
    Fe-NTA微柱纯化免疫沉淀(IP)洗脱物中磷酸肽的质谱分析
    作者:Ethan J. Sanford and Marcus B. Smolka日期:08/05/2021,浏览量:14,Q&A: 0

    Protein phosphorylation is a nearly universal signaling mechanism. To date, a number of proteomics tools have been developed to analyze phosphorylation. Phosphoproteome-wide analyses using whole cell extracts suffer from incomplete coverage, often

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    A Genetically Engineered Mouse Model of Venous Anomaly and Retinal Angioma-like Vascular Malformation
    静脉畸形和视网膜血管瘤样血管畸形的基因工程小鼠模型
    作者:Xudong Cao, Beibei Xu, Xiao Li, Taotao Li and Yulong He日期:08/05/2021,浏览量:15,Q&A: 0

    Characterization of key regulators in vein development will advance our understanding of mechanisms underlying venous anomalies and provide therapeutic targets for the treatment of vascular malformations. Here, we provide a detailed protocol for the

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    A Genetically Engineered Mouse Model of Venous Anomaly and Retinal Angioma-like Vascular Malformation
    静脉畸形和视网膜血管瘤样血管畸形的基因工程小鼠模型
    作者:Xudong Cao, Beibei Xu, Xiao Li, Taotao Li and Yulong He日期:08/05/2021,浏览量:15,Q&A: 0
    [Abstract]

    Characterization of key regulators in vein development will advance our understanding of mechanisms underlying venous anomalies and provide therapeutic targets for the treatment of vascular malformations. Here, we provide a detailed protocol for the generation of genetically engineered mouse models targeting the Tek gene for the analysis of vein

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    Fe-NTA Microcolumn Purification of Phosphopeptides from Immunoprecipitation (IP) Eluates for Mass Spectrometry Analysis
    Fe-NTA微柱纯化免疫沉淀(IP)洗脱物中磷酸肽的质谱分析
    作者:Ethan J. Sanford and Marcus B. Smolka日期:08/05/2021,浏览量:14,Q&A: 0
    [Abstract]

    Protein phosphorylation is a nearly universal signaling mechanism. To date, a number of proteomics tools have been developed to analyze phosphorylation. Phosphoproteome-wide analyses using whole cell extracts suffer from incomplete coverage, often missing phosphorylation events from low-abundance proteins. In order to increase coverage of

    ...
    Purification of Recombinant Wild Type and Mutant Ryanodine Receptors Expressed in HEK293 Cells
    HEK293细胞表达的重组野生型和突变型Ryanodine受体的纯化
    作者:Yifan Hu, Kavita A. Iyer, Ashok R. Nayak, Nagomi Kurebayashi, Takashi Murayama and Montserrat Samsó日期:08/05/2021,浏览量:21,Q&A: 0
    [Abstract]

    High quantities of purified ryanodine receptor (RyR), a large (2.26 MDa) intracellular homotetrameric membrane protein, can be obtained from heterologous expression in HEK293 cells and used for structure determination by cryo-EM. The advantage of using recombinant protein is that the variability due to post-translational modifications can be

    ...
    Purification and Cryo-electron Microscopy Analysis of Plant Mitochondrial Ribosomes
    植物线粒体核糖体的纯化和低温电镜分析
    作者:Florent Waltz, Philippe Giegé and Yaser Hashem日期:08/05/2021,浏览量:17,Q&A: 0
    [Abstract]

    Plants make up by far the largest part of biomass on Earth. They are the primary source of food and the basis of most drugs used for medicinal purposes. Similarly to all eukaryotes, plant cells also use mitochondria for energy production. Among mitochondrial gene expression processes, translation is the least understood; although, recent advances

    ...
    Purification of Mitochondrial Ribosomes with the Translocase Oxa1L from HEK Cells
    用转位酶Oxa1L纯化HEK细胞线粒体核糖体
    作者:Hanting Yang and Nirupa Desai日期:08/05/2021,浏览量:19,Q&A: 0
    [Abstract]

    Mitochondrial ribosomes (mitoribosomes) perform protein synthesis inside mitochondria, the organelles responsible for energy conversion and adenosine triphosphate (ATP) production in eukaryotic cells. To investigate their functions and structures, large-scale purification of intact mitoribosomes from mitochondria-rich animal tissues or HEK cells

    ...
    Cell-attached and Whole-cell Patch-clamp Recordings of Dopamine Neurons in the Substantia Nigra Pars Compacta of Mouse Brain Slices
    小鼠脑片黑质致密部多巴胺神经元的细胞贴附和全细胞膜片钳记录
    作者:Stefano Cattaneo, Maria Regoni, Jenny Sassone and Stefano Taverna日期:08/05/2021,浏览量:17,Q&A: 0
    [Abstract]

    The Substantia Nigra pars compacta (SNc) is a midbrain dopaminergic nucleus that plays a key role in modulating motor and cognitive functions. It is crucially involved in several disorders, particularly Parkinson’s disease, which is characterized by a progressive loss of SNc dopaminergic cells. Electrophysiological studies on SNc neurons are

    ...
    A Simple Spatial-independent Associative and Reversal Learning Task in Mice
    小鼠的一个简单的空间独立的联想和逆转学习任务
    作者:Fulvio Magara, Benjamin Boury-Jamot and Hanna Hörnberg日期:08/05/2021,浏览量:17,Q&A: 0
    [Abstract]

    The ability to adapt one's behavior in response to changing circumstances, or cognitive flexibility, is often altered in neuropsychiatric and neurodevelopmental conditions. In rodents, cognitive flexibility is frequently assessed using associative learning paradigms with a reversal component. The majority of existing protocols rely on

    ...
    Mouse Periosteal Cell Culture, in vitro Differentiation, and in vivo Transplantation in Tibial Fractures
    小鼠骨膜细胞培养、体外分化及胫骨骨折的体内移植
    作者:Simon Perrin, Anais Julien, Oriane Duchamp de Lageneste, Rana Abou-Khalil and Céline Colnot日期:08/05/2021,浏览量:44,Q&A: 0
    [Abstract]

    The periosteum covering the outer surface of bone contains skeletal stem/progenitor cells that can efficiently form cartilage and bone during bone repair. Several methods have been described to isolate periosteal cells based on bone scraping and/or enzymatic digestion. Here, we describe an explant culture method to isolate periosteum-derived

    ...
    Efficient and Rapid Analysis of Polysomes and Ribosomal Subunits in Cells and Tissues Using Ribo Mega-SEC
    利用Ribo Mega-SEC高效快速分析细胞和组织中的多聚体和核糖体亚基
    作者:Harunori Yoshikawa, Ramasubramanian Sundaramoorthy, Daniel Mariyappa, Hao Jiang and Angus I. Lamond日期:08/05/2021,浏览量:20,Q&A: 0
    [Abstract]

    Polysome profile analysis is a popular method for separating polysomes and ribosomal subunits and is typically achieved using a sucrose density gradient (SDG). This has remained the gold standard method since ribosomes were first discovered; however, this method is time-consuming and requires multiple steps from making the gradient and long

    ...
    Isolation and in vitro Culture of Mouse Oocytes
    小鼠卵母细胞的分离与体外培养
    作者:Jessica Greaney, Goutham Narayanan Subramanian, Yunan Ye and Hayden Homer日期:08/05/2021,浏览量:23,Q&A: 0
    [Abstract]

    Females are endowed at birth with a fixed reserve of oocytes, which declines both in quantity and quality with advancing age. Understanding the molecular mechanisms regulating oocyte quality is crucial for improving the chances of pregnancy success in fertility clinics. In vitro culture systems enable researchers to analyse important molecular and

    ...