哺乳纲

分类

    现刊
    Purification of Globular Actin from Rabbit Muscle and Pyrene Fluorescent Assays to Investigate Actin Dynamics in vitro
    从兔肌肉组织中纯化球状肌动蛋白并利用芘荧光分析实验体外检测肌动蛋白的动态变化
    作者:He Sun, Yuanyuan Luo and Yansong Miao日期:12/05/2018,浏览量:64,Q&A: 0
    Pyrene Fluorescent Assay is established to monitor the dynamic actin nucleation, elongation, capping and disassembly in vitro. This technique provides an easy handle procedure and straightforward visual data analysis. By coupling actin ...
    Ultrasound Guided Intra-thymic Injection to Track Recent Thymic Emigrants and Investigate T Cell Development
    超声引导胸腺内注射并追踪新近胸腺迁出细胞和T细胞发育观测
    作者:Haiguang Wang, David L. Owen, Lily J. Qian, Laura B. Chopp, Michael A. Farrar and Kristin A. Hogquist日期:12/05/2018,浏览量:77,Q&A: 0
    To track recent thymic emigrants (RTEs) or study T cell development in the thymus, intra-thymic injection of a cellular tag or precursor cells for various T cell lineages is often desired. However, the traditional surgical approach to expose the ...
    Cluster Analysis of Endogenous HER2 and HER3 Receptors in SKBR3 Cells
    在SKBR3细胞中内源性HER2和HER3受体的聚类分析
    The Human Epidermal Growth Factor Receptor (HER) family of receptor tyrosine kinases consists of four, single pass, transmembrane receptor homologs (HER1-4) that act to regulate many critical processes in normal and tumor cells. HER2 is ...
    Integrin Dependent RhoB Activation Assay Using Leukocytes
    白细胞内整合素依赖的RhoB激活实验
    作者:Janne Lehtonen, Katarzyna Potrzebowska, Malin Samuelsson and Lena Svensson日期:12/05/2018,浏览量:43,Q&A: 0
    To be able to migrate, leukocyte needs to re-use its adhesion molecules to move forward. These adhesion molecules are called integrins and are intracellularly transported via endocytosis and exocytosis in order to translocate to a new site on the ...
    Flow Cytometry Assay for Recycling of LFA-1 in T-lymphocytes
    流式细胞术检测T淋巴细胞中LFA-1的再循环
    作者:Katarzyna Potrzebowska, Janne Lehtonen, Malin Samuelsson and Lena Svensson日期:12/05/2018,浏览量:55,Q&A: 0
    To enable cells to move forward, cell surface integrins are internalized into an endosomal compartment and subsequently intracellularly transported to be re-exposed at a new site on the cell membrane. Leukocytes are the fastest migrating cell type ...
    Wheat Germ Agglutinin (WGA)-SDS-PAGE: A Novel Method for the Detection of O-GlcNAc-modified Proteins by Lectin Affinity Gel Electrophoresis
    小麦种子凝集素(WGA)-SDS-PAGE:一种利用凝集素亲和凝胶电泳对O-乙酰氨基葡萄糖修饰蛋白进行检测的新方法
    作者:Ko Fujioka, Yuji Kubota and Mutsuhiro Takekawa日期:12/05/2018,浏览量:97,Q&A: 0
    Diverse cytoplasmic and nuclear proteins dynamically change their molecular functions by O-linked β-N-acetylglucosamine (O-GlcNAc) modification on serine and/or threonine residues. Evaluation of the O-GlcNAcylation level of a specific protein, ...
    Purification of Globular Actin from Rabbit Muscle and Pyrene Fluorescent Assays to Investigate Actin Dynamics in vitro
    从兔肌肉组织中纯化球状肌动蛋白并利用芘荧光分析实验体外检测肌动蛋白的动态变化
    作者:He Sun, Yuanyuan Luo and Yansong Miao日期:12/05/2018,浏览量:64,Q&A: 0
    [Abstract] Pyrene Fluorescent Assay is established to monitor the dynamic actin nucleation, elongation, capping and disassembly in vitro. This technique provides an easy handle procedure and straightforward visual data analysis. By coupling actin purification and polymerization assays in this protocol, the readers could quickly get the affordable ...
    Ultrasound Guided Intra-thymic Injection to Track Recent Thymic Emigrants and Investigate T Cell Development
    超声引导胸腺内注射并追踪新近胸腺迁出细胞和T细胞发育观测
    作者:Haiguang Wang, David L. Owen, Lily J. Qian, Laura B. Chopp, Michael A. Farrar and Kristin A. Hogquist日期:12/05/2018,浏览量:77,Q&A: 0
    [Abstract] To track recent thymic emigrants (RTEs) or study T cell development in the thymus, intra-thymic injection of a cellular tag or precursor cells for various T cell lineages is often desired. However, the traditional surgical approach to expose the thymus for intra-thymic injection is time-consuming and can cause a high level of pain and stress to ...
    Cluster Analysis of Endogenous HER2 and HER3 Receptors in SKBR3 Cells
    在SKBR3细胞中内源性HER2和HER3受体的聚类分析
    [Abstract] The Human Epidermal Growth Factor Receptor (HER) family of receptor tyrosine kinases consists of four, single pass, transmembrane receptor homologs (HER1-4) that act to regulate many critical processes in normal and tumor cells. HER2 is overexpressed in many tumors, and the deregulated proliferation of cancerous cells is driven by cooperation with ...
    Integrin Dependent RhoB Activation Assay Using Leukocytes
    白细胞内整合素依赖的RhoB激活实验
    作者:Janne Lehtonen, Katarzyna Potrzebowska, Malin Samuelsson and Lena Svensson日期:12/05/2018,浏览量:43,Q&A: 0
    [Abstract] To be able to migrate, leukocyte needs to re-use its adhesion molecules to move forward. These adhesion molecules are called integrins and are intracellularly transported via endocytosis and exocytosis in order to translocate to a new site on the cell membrane. The intracellular transportation is regulated by different small GTPases including ...
    Flow Cytometry Assay for Recycling of LFA-1 in T-lymphocytes
    流式细胞术检测T淋巴细胞中LFA-1的再循环
    作者:Katarzyna Potrzebowska, Janne Lehtonen, Malin Samuelsson and Lena Svensson日期:12/05/2018,浏览量:55,Q&A: 0
    [Abstract] To enable cells to move forward, cell surface integrins are internalized into an endosomal compartment and subsequently intracellularly transported to be re-exposed at a new site on the cell membrane. Leukocytes are the fastest migrating cell type in the human body, which express the leukocyte-specific integrin LFA-1. Here, we describe a flow ...
    Wheat Germ Agglutinin (WGA)-SDS-PAGE: A Novel Method for the Detection of O-GlcNAc-modified Proteins by Lectin Affinity Gel Electrophoresis
    小麦种子凝集素(WGA)-SDS-PAGE:一种利用凝集素亲和凝胶电泳对O-乙酰氨基葡萄糖修饰蛋白进行检测的新方法
    作者:Ko Fujioka, Yuji Kubota and Mutsuhiro Takekawa日期:12/05/2018,浏览量:97,Q&A: 0
    [Abstract] Diverse cytoplasmic and nuclear proteins dynamically change their molecular functions by O-linked β-N-acetylglucosamine (O-GlcNAc) modification on serine and/or threonine residues. Evaluation of the O-GlcNAcylation level of a specific protein, however, needs multiple and time-consuming steps if using conventional methods (e.g., ...
    Purification of Soluble Recombinant Human Tau Protein from Bacteria Using Double-tag Affinity Purification
    双标签亲和纯化法从细菌中纯化人可溶重组Tau蛋白
    作者:Joseph McInnes, Lujia Zhou and Patrik Verstreken日期:11/20/2018,浏览量:333,Q&A: 0
    [Abstract] Dysfunction of the microtubule-associated protein Tau (encoded by the MAPT gene) has been implicated in more than twenty neurodegenerative diseases, including Alzheimer’s. As such, the physiological and disease-relevant functions of Tau have garnered great interest in the research community. One barrier hampering investigations into the ...
    A Functionally Robust Phenotypic Screen that Identifies Drug Resistance-associated Genes Using 3D Cell Culture
    利用3D细胞培养鉴定耐药相关基因的一种功能性稳健表型筛选
    作者:Sun-Young Lee and Mina J. Bissell日期:11/20/2018,浏览量:463,Q&A: 0
    [Abstract] Drug resistance is a major obstacle in cancer treatment: A case in point is the failure of cancer patients to respond to tyrosine kinase inhibitors (TKI) of EGFR, a receptor that is highly expressed in many cancers. Identification of the targets and delineation of mechanisms of drug resistance remain major challenges. Traditional pharmacological ...
    Use of Gas Chromatography to Quantify Short Chain Fatty Acids in the Serum, Colonic Luminal Content and Feces of Mice
    利用气相色谱法定量分析小鼠血清、结肠管腔内容物和粪便中短链脂肪酸
    [Abstract] Short-Chain Fatty Acids (SCFAs) are a product of the fermentation of resistant starches and dietary fibers by the gut microbiota. The most important SCFA are acetate (C2), propionate (C3) and butyrate (C4). These metabolites are formed and absorbed in the colon and then transported through the hepatic vein to the liver. SCFAs are more concentrated ...
    Electron Microscopic Detection of Proteins and Protein Complexes in Mammalian Cells Using APEX-tagged, Conditionally Stable Nanobodies
    利用APEX标记的条件性稳定纳米体电子显微检测哺乳动物细胞中的蛋白和蛋白复合物
    作者:Thomas E. Hall, James Rae, Nicholas Ariotti and Robert G. Parton日期:11/20/2018,浏览量:268,Q&A: 0
    [Abstract] The recent development of 3D electron microscopic techniques for cells and tissues has necessitated the development of new methods for the detection of proteins and protein-complexes in situ. The development of new genetic tags, such as the ascorbate peroxidase, APEX, for electron microscopic detection of tagged proteins has expanded the ...