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哺乳纲

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    现刊
    Detection of Intracellular Reduced (Catalytically Active) SHP-1 and Analyses of Catalytically Inactive SHP-1 after Oxidation by Pervanadate or H2O2
    细胞内还原的(具有催化活性的)SHP-1检测以及在经钒酸盐或H2O2氧化后无催化活性SHP-1的分析
    作者:Seeyoung Choi and Paul E. Love日期:01/05/2018,浏览量:174,Q&A: 0
    Oxidative inactivation of cysteine-dependent Protein Tyrosine Phosphatases (PTPs) by cellular reactive oxygen species (ROS) plays a critical role in regulating signal transduction in multiple cell types. The phosphatase activity of most PTPs depends ...
    Transplantation of Fecal Microbiota Shaped by Diet
    饮食经控制的粪便微生物群的移植
    作者:Keiran H. McLeod, Linda Mason and Eliana Mariño日期:01/05/2018,浏览量:90,Q&A: 0
    Alterations in diet and gut microbial ecology underlie the pathogenesis of type 1 diabetes (T1D). In the non-obese diabetic (NOD) mouse, we found high concentrations of bacterial metabolites acetate and butyrate in blood and faeces correlated with ...
    High Throughput NPY-Venus and Serotonin Secretion Assays for Regulated Exocytosis in Neuroendocrine Cells
    高通量NPY-Venus和5-羟色胺分泌实验分析神经内分泌细胞中被调控的胞吐作用
    作者:Xingmin Aaron Zhang and Thomas F.J. Martin日期:01/05/2018,浏览量:153,Q&A: 0
    Here we describe two assays to measure dense core vesicle (DCV) exocytosis-mediated cargo secretion in neuroendocrine cells. To conduct siRNA screens for novel genes in regulated DCV exocytosis, we developed a plate reader-based secretion assay ...
    Determining Ribosome Translational Status by Ribo-ELISA
    采用Ribo-ELISA检测核糖体的翻译状态
    作者:Amandine Bastide, Jonathan W. Yewdell and Alexandre David日期:01/05/2018,浏览量:258,Q&A: 0
    The Ribo-ELISA was originally developed to elucidate the basis for the ribopuromycylation method (RPM)-based detection of ribosome bound nascent chains. The Ribo-ELISA enables characterization of the translational status of ribosomes, and can be ...
    The RiboPuromycylation Method (RPM): an Immunofluorescence Technique to Map Translation Sites at the Sub-cellular Level
    RiboPuromycylation方法(RPM):一种在亚细胞水平绘制翻译位点图谱的免疫荧光技术
    作者:Amandine Bastide, Jonathan W. Yewdell and Alexandre David日期:01/05/2018,浏览量:131,Q&A: 0
    While isotopic labeling of amino acids remains the reference method in the field for quantifying translation rate, it does not provide any information on spatial localization of translation sites. The rationale behind developing the ...
    A Rodent Model for Chronic Brain Hypoperfusion Related Diseases: Permanent Bilateral Occlusion of the Common Carotid Arteries (2VO) in Rats
    慢性脑灌注不足相关疾病的啮齿动物模型:大鼠双侧颈总动脉永久性闭塞(2VO)
    作者:Mei-Ling Yan and Jing Ai日期:01/05/2018,浏览量:122,Q&A: 0
    Permanent occlusion of bilateral common carotid arteries (2VO) in rat is considered as a suitable animal model to mimic chronic brain hypoperfusion status, which is proved to be a risk factor to precede the Alzheimer’s disease or/and vascular ...
    Mutant Huntingtin Secretion in Neuro2A Cells and Rat Primary Cortical Neurons
    Neuro2A细胞和大鼠原代皮层神经元中突变亨廷顿蛋白的分泌
    作者:Katarina Trajkovic, Hyunkyung Jeong and Dimitri Krainc日期:01/05/2018,浏览量:111,Q&A: 0
    Quantitative analysis of proteins secreted from the cells poses a challenge due to their low abundance and the interfering presence of a large amount of bovine serum albumin (BSA) in the cell culture media. We established assays for detection of ...
    Visible Immunoprecipitation (VIP) Assay: a Simple and Versatile Method for Visual Detection of Protein-protein Interactions
    可视免疫沉淀(VIP)实验:一种简单通用的蛋白质 - 蛋白质相互作用可视化检测方法
    作者:Yohei Katoh, Kentaro Nakamura and Kazuhisa Nakayama日期:01/05/2018,浏览量:232,Q&A: 0
    The visible immunoprecipitation (VIP) assay is a convenient alternative to conventional co-immunoprecipitation (Katoh et al., 2015). By processing lysates from cells co-expressing GFP-fusion and RFP-fusion proteins for immunoprecipitation ...
    Detection of Intracellular Reduced (Catalytically Active) SHP-1 and Analyses of Catalytically Inactive SHP-1 after Oxidation by Pervanadate or H2O2
    细胞内还原的(具有催化活性的)SHP-1检测以及在经钒酸盐或H2O2氧化后无催化活性SHP-1的分析
    作者:Seeyoung Choi and Paul E. Love日期:01/05/2018,浏览量:174,Q&A: 0
    [Abstract] Oxidative inactivation of cysteine-dependent Protein Tyrosine Phosphatases (PTPs) by cellular reactive oxygen species (ROS) plays a critical role in regulating signal transduction in multiple cell types. The phosphatase activity of most PTPs depends upon a ‘signature’ cysteine residue within the catalytic domain that is maintained in the ...
    Transplantation of Fecal Microbiota Shaped by Diet
    饮食经控制的粪便微生物群的移植
    作者:Keiran H. McLeod, Linda Mason and Eliana Mariño日期:01/05/2018,浏览量:90,Q&A: 0
    [Abstract] Alterations in diet and gut microbial ecology underlie the pathogenesis of type 1 diabetes (T1D). In the non-obese diabetic (NOD) mouse, we found high concentrations of bacterial metabolites acetate and butyrate in blood and faeces correlated with protection from disease. We reconstituted germ free (GF) NOD mice with fecal bacteria from protected ...
    High Throughput NPY-Venus and Serotonin Secretion Assays for Regulated Exocytosis in Neuroendocrine Cells
    高通量NPY-Venus和5-羟色胺分泌实验分析神经内分泌细胞中被调控的胞吐作用
    作者:Xingmin Aaron Zhang and Thomas F.J. Martin日期:01/05/2018,浏览量:153,Q&A: 0
    [Abstract] Here we describe two assays to measure dense core vesicle (DCV) exocytosis-mediated cargo secretion in neuroendocrine cells. To conduct siRNA screens for novel genes in regulated DCV exocytosis, we developed a plate reader-based secretion assay using DCV cargo, NPY-Venus, and an orthogonal 3H-serotonin secretion assay. The NPY-Venus ...
    Determining Ribosome Translational Status by Ribo-ELISA
    采用Ribo-ELISA检测核糖体的翻译状态
    作者:Amandine Bastide, Jonathan W. Yewdell and Alexandre David日期:01/05/2018,浏览量:258,Q&A: 0
    [Abstract] The Ribo-ELISA was originally developed to elucidate the basis for the ribopuromycylation method (RPM)-based detection of ribosome bound nascent chains. The Ribo-ELISA enables characterization of the translational status of ribosomes, and can be applied to the discovery of super-ribosomal complexes with novel ribosome associated macromolecules ...
    The RiboPuromycylation Method (RPM): an Immunofluorescence Technique to Map Translation Sites at the Sub-cellular Level
    RiboPuromycylation方法(RPM):一种在亚细胞水平绘制翻译位点图谱的免疫荧光技术
    作者:Amandine Bastide, Jonathan W. Yewdell and Alexandre David日期:01/05/2018,浏览量:131,Q&A: 0
    [Abstract] While isotopic labeling of amino acids remains the reference method in the field for quantifying translation rate, it does not provide any information on spatial localization of translation sites. The rationale behind developing the ribopuromycylation method (RPM) was primarily to map translation sites at the sub-cellular level while avoiding ...
    A Rodent Model for Chronic Brain Hypoperfusion Related Diseases: Permanent Bilateral Occlusion of the Common Carotid Arteries (2VO) in Rats
    慢性脑灌注不足相关疾病的啮齿动物模型:大鼠双侧颈总动脉永久性闭塞(2VO)
    作者:Mei-Ling Yan and Jing Ai日期:01/05/2018,浏览量:122,Q&A: 0
    [Abstract] Permanent occlusion of bilateral common carotid arteries (2VO) in rat is considered as a suitable animal model to mimic chronic brain hypoperfusion status, which is proved to be a risk factor to precede the Alzheimer’s disease or/and vascular dementia. In this protocol, we describe how to successfully ligate the bilateral common carotid arteries ...
    Mutant Huntingtin Secretion in Neuro2A Cells and Rat Primary Cortical Neurons
    Neuro2A细胞和大鼠原代皮层神经元中突变亨廷顿蛋白的分泌
    作者:Katarina Trajkovic, Hyunkyung Jeong and Dimitri Krainc日期:01/05/2018,浏览量:111,Q&A: 0
    [Abstract] Quantitative analysis of proteins secreted from the cells poses a challenge due to their low abundance and the interfering presence of a large amount of bovine serum albumin (BSA) in the cell culture media. We established assays for detection of mutant huntingtin (mHtt) secreted from Neuro2A cell line stably expressing mHtt and rat primary ...
    Visible Immunoprecipitation (VIP) Assay: a Simple and Versatile Method for Visual Detection of Protein-protein Interactions
    可视免疫沉淀(VIP)实验:一种简单通用的蛋白质 - 蛋白质相互作用可视化检测方法
    作者:Yohei Katoh, Kentaro Nakamura and Kazuhisa Nakayama日期:01/05/2018,浏览量:232,Q&A: 0
    [Abstract] The visible immunoprecipitation (VIP) assay is a convenient alternative to conventional co-immunoprecipitation (Katoh et al., 2015). By processing lysates from cells co-expressing GFP-fusion and RFP-fusion proteins for immunoprecipitation with GST-tagged anti-GFP Nanobody and glutathione-Sepharose beads, protein-protein interactions can ...
    Oral Microbiome Characterization in Murine Models
    模式生物小鼠口腔微生物组鉴定
    作者:Loreto Abusleme, Bo-Young Hong, Anilei Hoare, Joanne E. Konkel, Patricia I. Diaz and Niki M. Moutsopoulos日期:12/20/2017,浏览量:498,Q&A: 0
    [Abstract] The oral microbiome has been implicated as a trigger for immune responsiveness in the oral cavity, particularly in the setting of the inflammatory disease periodontitis. The protocol presented here is aimed at characterizing the oral microbiome in murine models at steady state and during perturbations of immunity or physiology. Herein, we describe ...
    A General Method for Intracellular Protein Delivery through ‘E-tag’ Protein Engineering and Arginine Functionalized Gold Nanoparticles
    通过'E标签'蛋白质工程和精氨酸功能化金纳米粒子进行细胞内蛋白质递送的通用方法
    作者:Rubul Mout and Vincent M. Rotello日期:12/20/2017,浏览量:485,Q&A: 0
    [Abstract] In this protocol, we describe a method for direct cytosolic protein delivery that avoids endosomal entrapment of the delivered proteins. We achieved this by tagging the desired protein with an oligo glutamic acid tag (E-tag), and subsequently using carrier gold nanoparticles to deliver these E-tagged proteins. When E-tagged proteins and ...