哺乳纲

分类

    现刊
    Multiplex T-cell Stimulation Assay Utilizing a T-cell Activation Reporter-based Detection System
    利用基于T细胞活化报告基因的检测系统进行多重T细胞刺激测定
    作者:Laurie G. Landry, Sarah E. Mann, Amanda M. Anderson and Maki Nakayama日期:01/20/2021,浏览量:110,Q&A: 0

    Immune tolerance and response are both largely driven by the interactions between the major histocompatibility complex (MHC) expressed by antigen presenting cells (APCs), T-cell receptors (TCRs) on T-cells, and their cognate antigens. Disordered

    ...
    A mant-GDP Dissociation Assay to Compare the Guanine Nucleotide Binding Preference of Small GTPases
    用Mant-GDP分离实验比较小GTP蛋白的鸟嘌呤核苷酸的结合偏好
    作者:Yuping Tan and Qingxiang Sun日期:01/20/2021,浏览量:110,Q&A: 0

    Small GTPases are cellular switches that are switched on when bound to GTP and switched off when bound to GDP. Different small GTPase proteins or those with mutations may bind to GTP or GDP with different relative affinities. However, small GTPases

    ...
    Polyamine Transport Assay Using Reconstituted Yeast Membranes
    利用重构酵母细胞膜的多胺转运实验
    作者:Sarah van Veen, Shaun Martin, Marleen Schuermans and Peter Vangheluwe日期:01/20/2021,浏览量:159,Q&A: 0

    ATP13A2/PARK9 is a late endo-/lysosomal P5B transport ATPase that is associated with several neurodegenerative disorders. We recently characterized ATP13A2 as a lysosomal polyamine exporter, which sheds light on the molecular identity of the unknown

    ...
    Imaging of Human Cancer Cells in 3D Collagen Matrices
    人类癌细胞的3D胶原基质成像
    作者:Karin Pfisterer, Brooke Lumicisi and Maddy Parsons日期:01/20/2021,浏览量:139,Q&A: 0

    Research on cell migration and interactions with the extracellular matrix (ECM) was mostly focused on 2D surfaces in the past. Many recent studies have highlighted differences in migratory behaviour of cells on 2D surfaces compared to complex cell

    ...
    Characterization of Hippocampal Adult-borne Granule Cells in a Transient Cerebral Ischemia Model
    海马成体新生颗粒细胞在短暂性脑缺血模型中的特征
    作者:Mihai Ceanga, Madlen Guenther, Ina Ingrisch and Albrecht Kunze日期:01/20/2021,浏览量:57,Q&A: 0

    Long-term consequences of stroke significantly impair the quality of life in a growing population of stroke survivors. Hippocampal adult neurogenesis has been hypothesized to play a role in the pathophysiology of cognitive and neuropsychiatric

    ...
    Low- and High-resolution Dynamic Analyses for Magnetic Resonance Spectroscopy Data
    磁共振光谱数据的低高分辨率动态分析
    作者:Reuben Rideaux日期:01/20/2021,浏览量:61,Q&A: 0

    Magnetic resonance spectroscopy (MRS) can be used to measure in vivo concentrations of neurometabolites. This information can be used to identify neurotransmitter involvement in healthy (e.g., perceptual and cognitive processes) and unhealthy brain

    ...
    Histological and Immunohistochemical Examination of Stem Cell Proliferation and Reepithelialization in the Wounded Skin
    损伤皮肤干细胞增殖和再生的组织学和免疫组化检测
    作者:Rupali Gund, Ravindra Zirmire, Haarshaadri J., Gaurav Kansagara and Colin Jamora日期:01/20/2021,浏览量:72,Q&A: 0

    The skin is the largest organ that protects our body from the external environment and it is constantly exposed to pathogenic insults and injury. Repair of damage to this organ is carried out by a complex process involving three overlapping phases

    ...
    Preparation of Synaptoneurosomes to Study the Synapse in the Murine Cerebral Cortex
    用于研究小鼠大脑皮层突触的突触神经小体的制备
    作者:Ariel Diaz, Enrique Torre and Manuel Yepes日期:01/20/2021,浏览量:72,Q&A: 0

    The synapse is a complex structure where the transmission of information takes place. Synaptic dysfunction is one of the earliest pathophysiological events in several diseases, such as traumatic brain injury, cerebral ischemia, and neurodegenerative

    ...
    Reverse Phase-high-performance Liquid Chromatography (RP-HPLC) Analysis of Globin Chains from Human Erythroid Cells
    人类红细胞珠蛋白链的反相高效液相色谱(RP-HPLC)分析
    作者:Anne Chalumeau, Giacomo Frati, Elisa Magrin and Annarita Miccio日期:01/20/2021,浏览量:121,Q&A: 0

    β-hemoglobinopathies are severe genetic disorders characterized either by the abnormal synthesis of the adult β-globin chains of the hemoglobin (Hb) tetramer (βS-globin chains) in sickle cell disease (SCD) or by the reduced

    ...
    Reverse Phase-high-performance Liquid Chromatography (RP-HPLC) Analysis of Globin Chains from Human Erythroid Cells
    人类红细胞珠蛋白链的反相高效液相色谱(RP-HPLC)分析
    作者:Anne Chalumeau, Giacomo Frati, Elisa Magrin and Annarita Miccio日期:01/20/2021,浏览量:121,Q&A: 0
    [Abstract]

    β-hemoglobinopathies are severe genetic disorders characterized either by the abnormal synthesis of the adult β-globin chains of the hemoglobin (Hb) tetramer (βS-globin chains) in sickle cell disease (SCD) or by the reduced β-globin production in β-thalassemia. The identification and quantification of globin chains are

    ...
    Preparation of Synaptoneurosomes to Study the Synapse in the Murine Cerebral Cortex
    用于研究小鼠大脑皮层突触的突触神经小体的制备
    作者:Ariel Diaz, Enrique Torre and Manuel Yepes日期:01/20/2021,浏览量:72,Q&A: 0
    [Abstract]

    The synapse is a complex structure where the transmission of information takes place. Synaptic dysfunction is one of the earliest pathophysiological events in several diseases, such as traumatic brain injury, cerebral ischemia, and neurodegenerative diseases. Thus, a methodology to study synaptic structure and function is crucial for the

    ...
    Histological and Immunohistochemical Examination of Stem Cell Proliferation and Reepithelialization in the Wounded Skin
    损伤皮肤干细胞增殖和再生的组织学和免疫组化检测
    作者:Rupali Gund, Ravindra Zirmire, Haarshaadri J., Gaurav Kansagara and Colin Jamora日期:01/20/2021,浏览量:72,Q&A: 0
    [Abstract]

    The skin is the largest organ that protects our body from the external environment and it is constantly exposed to pathogenic insults and injury. Repair of damage to this organ is carried out by a complex process involving three overlapping phases of inflammation, proliferation and remodeling. Histological analysis of wounded skin is a convenient

    ...
    Low- and High-resolution Dynamic Analyses for Magnetic Resonance Spectroscopy Data
    磁共振光谱数据的低高分辨率动态分析
    作者:Reuben Rideaux日期:01/20/2021,浏览量:61,Q&A: 0
    [Abstract]

    Magnetic resonance spectroscopy (MRS) can be used to measure in vivo concentrations of neurometabolites. This information can be used to identify neurotransmitter involvement in healthy (e.g., perceptual and cognitive processes) and unhealthy brain function (e.g., neurological and psychiatric illnesses). The standard approach for analyzing MRS

    ...
    Characterization of Hippocampal Adult-borne Granule Cells in a Transient Cerebral Ischemia Model
    海马成体新生颗粒细胞在短暂性脑缺血模型中的特征
    作者:Mihai Ceanga, Madlen Guenther, Ina Ingrisch and Albrecht Kunze日期:01/20/2021,浏览量:57,Q&A: 0
    [Abstract]

    Long-term consequences of stroke significantly impair the quality of life in a growing population of stroke survivors. Hippocampal adult neurogenesis has been hypothesized to play a role in the pathophysiology of cognitive and neuropsychiatric long-term sequelae of stroke. Reliable animal models of stroke are paramount to understanding their

    ...
    Imaging of Human Cancer Cells in 3D Collagen Matrices
    人类癌细胞的3D胶原基质成像
    作者:Karin Pfisterer, Brooke Lumicisi and Maddy Parsons日期:01/20/2021,浏览量:139,Q&A: 0
    [Abstract]

    Research on cell migration and interactions with the extracellular matrix (ECM) was mostly focused on 2D surfaces in the past. Many recent studies have highlighted differences in migratory behaviour of cells on 2D surfaces compared to complex cell migration modes in 3D environments. When embedded in 3D matrices, cells constantly sense the

    ...
    Polyamine Transport Assay Using Reconstituted Yeast Membranes
    利用重构酵母细胞膜的多胺转运实验
    作者:Sarah van Veen, Shaun Martin, Marleen Schuermans and Peter Vangheluwe日期:01/20/2021,浏览量:159,Q&A: 0
    [Abstract]

    ATP13A2/PARK9 is a late endo-/lysosomal P5B transport ATPase that is associated with several neurodegenerative disorders. We recently characterized ATP13A2 as a lysosomal polyamine exporter, which sheds light on the molecular identity of the unknown mammalian polyamine transport system. Here, we describe step by step a protocol to measure

    ...
    A mant-GDP Dissociation Assay to Compare the Guanine Nucleotide Binding Preference of Small GTPases
    用Mant-GDP分离实验比较小GTP蛋白的鸟嘌呤核苷酸的结合偏好
    作者:Yuping Tan and Qingxiang Sun日期:01/20/2021,浏览量:110,Q&A: 0
    [Abstract]

    Small GTPases are cellular switches that are switched on when bound to GTP and switched off when bound to GDP. Different small GTPase proteins or those with mutations may bind to GTP or GDP with different relative affinities. However, small GTPases generally have very high affinities for guanine nucleotides, rendering it difficult to compare the

    ...
    Multiplex T-cell Stimulation Assay Utilizing a T-cell Activation Reporter-based Detection System
    利用基于T细胞活化报告基因的检测系统进行多重T细胞刺激测定
    作者:Laurie G. Landry, Sarah E. Mann, Amanda M. Anderson and Maki Nakayama日期:01/20/2021,浏览量:110,Q&A: 0
    [Abstract]

    Immune tolerance and response are both largely driven by the interactions between the major histocompatibility complex (MHC) expressed by antigen presenting cells (APCs), T-cell receptors (TCRs) on T-cells, and their cognate antigens. Disordered interactions cause the pathogenesis of autoimmune diseases such as type 1 diabetes. Therefore, the

    ...
    A Parkinson’s Disease-relevant Mitochondrial and Neuronal Morphology High-throughput Screening Assay in LUHMES Cells
    LUHMES细胞中与帕金森氏病相关的线粒体和神经元形态学高通量筛选分析
    作者:Tom Leah, Irina Vazquez-Villaseñor, Laura Ferraiuolo, Stephen B Wharton and Heather Mortiboys日期:01/05/2021,浏览量:473,Q&A: 0
    [Abstract]

    Parkinson’s disease is a devastating neurodegenerative disorder affecting 2-3% of the population over 65 years of age. There is currently no disease-modifying treatment. One of the predominant pathological features of Parkinson’s disease is mitochondrial dysfunction, and much work has aimed to identify therapeutic compounds which can

    ...