原核生物

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    现刊
    Quantitative Transformation Efficiency Assay for Bacillus subtilis
    枯草芽孢杆菌转化效率的定量测定
    作者:Christian L. Loyo and Briana M. Burton日期:12/05/2018,浏览量:136,Q&A: 0
    Bacillus subtilis (B. subtilis) is a model Gram-positive organism used to study a variety of physiological states and stress responses, one of which is the development of competence. Competence refers to the physiological state of ...
    Quantitative Transformation Efficiency Assay for Bacillus subtilis
    枯草芽孢杆菌转化效率的定量测定
    作者:Christian L. Loyo and Briana M. Burton日期:12/05/2018,浏览量:136,Q&A: 0
    [Abstract] Bacillus subtilis (B. subtilis) is a model Gram-positive organism used to study a variety of physiological states and stress responses, one of which is the development of competence. Competence refers to the physiological state of a cell which allows it to be transformed naturally. Through induction of competence, the efficiency ...
    Purification of Soluble Recombinant Human Tau Protein from Bacteria Using Double-tag Affinity Purification
    双标签亲和纯化法从细菌中纯化人可溶重组Tau蛋白
    作者:Joseph McInnes, Lujia Zhou and Patrik Verstreken日期:11/20/2018,浏览量:333,Q&A: 0
    [Abstract] Dysfunction of the microtubule-associated protein Tau (encoded by the MAPT gene) has been implicated in more than twenty neurodegenerative diseases, including Alzheimer’s. As such, the physiological and disease-relevant functions of Tau have garnered great interest in the research community. One barrier hampering investigations into the ...
    Extraction and Analysis of Bacterial Teichoic Acids
    细菌磷壁酸的提取与分析
    作者:Kelvin Kho and Timothy C. Meredith日期:11/05/2018,浏览量:258,Q&A: 0
    [Abstract] Teichoic acids (TA) are anionic polymers comprised of polyol phosphate repeat units that are abundant in the cell wall of Gram-positive bacteria. Both wall teichoic acid (WTA) and lipoteichoic acid (LTA) play important roles in regulating cell wall remodeling as well as conferring antibiotic resistance. To analyze TA, we describe a polyacrylamide ...
    Kinetic Characterization of the Shigella Type Three Secretion System ATPase Spa47 Using α-32P ATP
    利用α-32P ATP研究志贺氏菌的III型分泌系统ATP酶Spa47的动力学表征
    作者:Heather B. Case and Nicholas E. Dickenson日期:11/05/2018,浏览量:189,Q&A: 0
    [Abstract] ATPases represent a diverse class of enzymes that utilize ATP hydrolysis to support critical biological functions such as driving ion pumps, providing mechanical work, unfolding/folding proteins, and supporting otherwise thermodynamically unfavorable chemical reactions. We have recently shown that the Shigella protein Spa47 is an ATPase ...
    An Innovative Approach to Study Ralstonia solanacearum Pathogenicity in 6 to 7 Days Old Tomato Seedlings by Root Dip Inoculation
    一种通过根部浸渍接种研究6-7天番茄幼苗青枯雷尔氏菌致病性的新方法
    作者:Niraj Singh, Rahul Kumar and Suvendra Kumar Ray日期:11/05/2018,浏览量:285,Q&A: 0
    [Abstract] Ralstonia solanacearum (F1C1) is a Gram-negative plant pathogenic bacterium that causes lethal wilt disease in a wide range of plant species. This pathogen is very well known for its unpredictable behavior during infection and wilting its host. Because of its mysterious infection behavior, virulence and pathogenicity standardization are ...
    Microtitre Plate Based Cell-SELEX Method
    基于微量滴定板的Cell-SELEX技术
    作者:Munish Shorie and Harmanjit Kaur日期:10/20/2018,浏览量:500,Q&A: 0
    [Abstract] Aptamers have emerged as a novel category in the field of bioreceptors due to their wide applications ranging from biosensing to therapeutics. Several variations of their screening process, called SELEX have been reported which can yield sequences with desired properties needed for their final use. We report a facile microtiter plate-based ...
    Assessing Membrane Fluidity and Visualizing Fluid Membrane Domains in Bacteria Using Fluorescent Membrane Dyes
    利用荧光膜染料评估膜流动性以及观察细菌中的流体膜结构区域
    作者:Michaela Wenzel, Norbert O. E. Vischer, Henrik Strahl and Leendert W. Hamoen日期:10/20/2018,浏览量:411,Q&A: 0
    [Abstract] Membrane fluidity is a key parameter of bacterial membranes that undergoes quick adaptation in response to environmental challenges and has recently emerged as an important factor in the antibacterial mechanism of membrane-targeting antibiotics. The specific level of membrane fluidity is not uniform across the bacterial cell membrane. Rather, ...
    A Method for SUMO Modification of Proteins in vitro
    体外蛋白SUMO化修饰的方法
    作者:Christine C. Lee, Bing Li, Hongtao Yu and Michael J. Matunis日期:10/05/2018,浏览量:516,Q&A: 0
    [Abstract] The Small Ubiquitin-related Modifier (SUMO) is a protein that is post-translationally added to and reversibly removed from other proteins in eukaryotic cells. SUMO and enzymes of the SUMO pathway are well conserved from yeast to humans and SUMO modification regulates a variety of essential cellular processes including transcription, chromatin ...
    CRISPR/Cas9-mediated ssDNA Recombineering in Corynebacterium glutamicum
    谷氨酸棒状杆菌中CRISPR/Cas9介导的ssDNA重组工程技术
    作者:Jiao Liu, Yu Wang, Ping Zheng and Jibin Sun日期:10/05/2018,浏览量:320,Q&A: 0
    [Abstract] Corynebacterium glutamicum is a versatile workhorse for industrial bioproduction of many kinds of chemicals and fuels, notably amino acids. Development of advanced genetic engineering tools is urgently demanded for systems metabolic engineering of C. glutamicum. Recently unveiled clustered regularly interspaced short palindromic ...
    Platelet Migration and Bacterial Trapping Assay under Flow
    流动条件下血小板迁移和细菌捕获检测
    作者:Shuxia Fan, Michael Lorenz, Steffen Massberg and Florian Gaertner日期:09/20/2018,浏览量:529,Q&A: 0
    [Abstract] Blood platelets are critical for hemostasis and thrombosis, but also play diverse roles during immune responses. We have recently reported that platelets migrate at sites of infection in vitro and in vivo. Importantly, platelets use their ability to migrate to collect and bundle fibrin (ogen)-bound bacteria accomplishing ...