原核生物

分类

    现刊
    Preparation of Yeast tRNA Sample for NMR Spectroscopy
    酵母tRNA样品的核磁共振制备
    作者:Marjorie Catala, Alexandre Gato, Carine Tisné and Pierre Barraud日期:06/20/2020,浏览量:457,Q&A: 0
    [Abstract] Transfer RNAs (tRNAs) are heavily decorated with post-transcriptional modifications during their biosynthesis. To fulfil their functions within cells, tRNAs undergo a tightly controlled biogenesis process leading to the formation of mature tRNAs. In addition, functions of tRNAs are often modulated by their modifications. Although the biological ...
    Site-specific DNA Mapping of Protein Binding Orientation Using Azidophenacyl Bromide (APB)
    叠氮苯酰溴(APB)定位蛋白质结合方向的DNA图谱
    作者:Himasha M. Perera and Michael A. Trakselis日期:06/20/2020,浏览量:638,Q&A: 0
    [Abstract] The orientation of a DNA-binding protein bound on DNA is determinative in directing the assembly of other associated proteins in the complex for enzymatic action. As an example, in a replisome, the orientation of the DNA helicase at the replication fork directs the assembly of the other associated replisome proteins. We have recently determined ...
    Electrophoretic Mobility Shift Assay of in vitro Phosphorylated RNA Polymerase II Carboxyl-terminal Domain Substrates
    体外磷酸化的RNA聚合酶II羧基末端域底物的电泳迁移率测定
    作者:Joshua E. Mayfield, Seema Irani and Yan Zhang日期:06/20/2020,浏览量:648,Q&A: 0
    [Abstract] Eukaryotic RNA polymerase II transcribes all protein-coding mRNAs and is highly regulated. A key mechanism directing RNA polymerase II and facilitating the co-transcriptional processing of mRNAs is the phosphorylation of its highly repetitive carboxyl-terminal domain (CTD) of its largest subunit, RPB1, at specific residues. A variety of techniques ...
    Flow Cytometry Analysis and Fluorescence-activated Cell Sorting of Myeloid Cells from Lung and Bronchoalveolar Lavage Samples from Mycobacterium tuberculosis-infected Mice
    结核分枝杆菌感染小鼠肺和支气管肺泡灌洗样品中髓样细胞的流式细胞仪分析和荧光激活细胞分选
    作者:Alissa C. Rothchild, Dat Mai, Alan Aderem and Alan H. Diercks日期:05/20/2020,浏览量:856,Q&A: 0
    [Abstract] Mycobacterium tuberculosis (Mtb) is transmitted by aerosol and can cause serious bacterial infection in the lung that can be fatal if left untreated. Mtb is now the leading cause of death worldwide by an infectious agent. Characterizing the early events of in vivo infection following aerosol challenge is critical for ...
    Purification of Protein-complexes from the Cyanobacterium Synechocystis sp. PCC 6803 Using FLAG-affinity Chromatography
    Cyanobacterium Synechocystis sp. PCC 6803 中蛋白混合物的标记亲和层析纯化方法
    作者:Minna M. Koskela, Petra Skotnicová, Éva Kiss and Roman Sobotka日期:05/20/2020,浏览量:604,Q&A: 0
    [Abstract] Exploring the structure and function of protein complexes requires their isolation in the native state–a task that is made challenging when studying labile and/or low abundant complexes. The difficulties in preparing membrane-protein complexes are especially notorious. The cyanobacterium Synechocystis sp. PCC 6803 is a widely used model ...
    Molecular Size Analysis of Recombinant Importin-histone Complexes Using Analytical Ultracentrifugation
    重组输入蛋白-组蛋白配合物分子大小的超离心分析
    作者:Abhilash Padavannil, Chad A. Brautigam and Yuh Min Chook日期:05/20/2020,浏览量:532,Q&A: 0
    [Abstract] Histones constitute the protein components of nucleosomes. Despite their small sizes, histones do not diffuse through the nuclear pore complex. Instead, they are transported to the nucleus by importins, either alone or in complex with histone chaperones. Determining the molecular size of the importin-histone complexes is key to understanding the ...
    In vitro Assay for Bacterial Membrane Protein Integration into Proteoliposomes
    细菌膜蛋白在蛋白质脂质体中的体外整合
    作者:Hanako Nishikawa, Masaru Sasaki and Ken-ichi Nishiyama日期:05/20/2020,浏览量:741,Q&A: 0
    [Abstract] It is important to experimentally determine how membrane proteins are integrated into biomembranes to unveil the roles of the integration factors, and to understand the functions and structures of membrane proteins. We have developed a reconstitution system for membrane protein integration in E. coli using purified factors, in which the ...
    Confocal and Super-resolution Imaging of RNA in Live Bacteria Using a Fluorogenic Silicon Rhodamine-binding Aptamer
    含氟硅-罗丹明适配体用于活性菌中RNA的激光共聚焦高分辨率成像
    作者:Regina Wirth, Peng Gao, G. Ulrich Nienhaus, Murat Sunbul and Andres Jäschke日期:05/05/2020,浏览量:1108,Q&A: 0
    [Abstract] Genetically encoded light-up RNA aptamers have been shown to be promising tools for the visualization of RNAs in living cells, helping us to advance our understanding of the broad and complex life of RNA. Although a handful of light-up aptamers spanning the visible wavelength region have been developed, none of them have yet been reported to be ...
    A Sensitive Coupled Enzyme Assay for Measuring Kinase and ATPase Kinetics Using ADP-Specific Hexokinase
    使用ADP特异性己糖激酶测量激酶和ATPase动力学的灵敏耦合酶活测定
    作者:Ciaran R. McFarlane and James W. Murray日期:05/05/2020,浏览量:623,Q&A: 0
    [Abstract] Kinases and ATPases perform essential biological functions in metabolism and regulation. Activity of these enzymes is commonly measured by coupling ATP consumption to the synthesis of a detectable product. For most assay systems the ATP concentration during the reaction is unknown, compromising the precision of the assay.

    Using the ...
    Sequence Alignment Using Machine Learning for Accurate Template-based Protein Structure Prediction
    基于模板的蛋白质结构精确预测的机器学习序列比对
    作者:Shuichiro Makigaki and Takashi Ishida日期:05/05/2020,浏览量:786,Q&A: 0
    [Abstract] Template-based modeling, the process of predicting the tertiary structure of a protein by using homologous protein structures, is useful when good templates can be available. Indeed, modern homology detection methods can find remote homologs with high sensitivity. However, the accuracy of template-based models generated from the ...