原核生物

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    现刊
    Hypochlorous Acid Staining with R19-S in the Drosophila Intestine upon Ingestion of Opportunistic Bacteria
    果蝇肠道摄入外来细菌后次氯酸R19-S染色
    作者:Salma Hachfi, Olivia Benguettat and Armel Gallet日期:05/20/2019,浏览量:94,Q&A: 0
    The intestine is endowed with an innate immune system that is required to fight any exogenous bacteria that are swallowed along with the food. The first line of defense that is mounted by the gut epithelium is the release of immune Reactive Oxygen ...
    Biofilm Formation Assay in Pseudomonas syringae
    丁香假单胞菌的生物膜形成实验
    作者:Xiaolong Shao, Yingpeng Xie, Yingchao Zhang and Xin Deng日期:05/20/2019,浏览量:197,Q&A: 0
    Pseudomonas syringae is a model plant pathogen that infects more than 50 plant species worldwide, thus leading to significant yield loss. Pseudomonas biofilm always adheres to the surfaces of medical devices or host cells, thereby ...
    An Improved Bioassay to Study Arabidopsis Induced Systemic Resistance (ISR) Against Bacterial Pathogens and Insect Pests
    一种的可用于检测拟南芥中细菌性病原体和害虫诱导的系统抗性的改进生物学方法
    作者:Nicolás M. Cecchini, Yi Song, Suruchi Roychoudhry, Jean T. Greenberg and Cara H. Haney日期:05/20/2019,浏览量:420,Q&A: 0
    The plant immune system is essential for plants to perceive and defend against bacterial, fungal and insect pests and pathogens. Induced systemic resistance (ISR) is a systemic immune response that occurs upon root colonization by beneficial ...
    Assembly of a Custom-made Device to Study Spreading Patterns of Pseudomonas putida Biofilms
    用于恶臭假单胞菌生物膜扩散模式研究的定制设备的组装
    作者:David R. Espeso, Esteban Martínez-García and Víctor de Lorenzo日期:05/20/2019,浏览量:172,Q&A: 0
    Biofilms are bacterial communities in the shape of exopolysaccharide matrix-encased aggregates attached onto interphases able to resist environmental aggressions. The development of bacteria in the shape of biofilms deeply affects the performance of ...
    Hypochlorous Acid Staining with R19-S in the Drosophila Intestine upon Ingestion of Opportunistic Bacteria
    果蝇肠道摄入外来细菌后次氯酸R19-S染色
    作者:Salma Hachfi, Olivia Benguettat and Armel Gallet日期:05/20/2019,浏览量:94,Q&A: 0
    [Abstract] The intestine is endowed with an innate immune system that is required to fight any exogenous bacteria that are swallowed along with the food. The first line of defense that is mounted by the gut epithelium is the release of immune Reactive Oxygen Species (ROS), such as hypochlorous acid (HOCl), into the lumen. HOCl is produced within 1.5 h of ...
    Biofilm Formation Assay in Pseudomonas syringae
    丁香假单胞菌的生物膜形成实验
    作者:Xiaolong Shao, Yingpeng Xie, Yingchao Zhang and Xin Deng日期:05/20/2019,浏览量:197,Q&A: 0
    [Abstract] Pseudomonas syringae is a model plant pathogen that infects more than 50 plant species worldwide, thus leading to significant yield loss. Pseudomonas biofilm always adheres to the surfaces of medical devices or host cells, thereby contributing to infection. Biofilm formation can be visualized on numerous matrixes, including ...
    An Improved Bioassay to Study Arabidopsis Induced Systemic Resistance (ISR) Against Bacterial Pathogens and Insect Pests
    一种的可用于检测拟南芥中细菌性病原体和害虫诱导的系统抗性的改进生物学方法
    作者:Nicolás M. Cecchini, Yi Song, Suruchi Roychoudhry, Jean T. Greenberg and Cara H. Haney日期:05/20/2019,浏览量:420,Q&A: 0
    [Abstract] The plant immune system is essential for plants to perceive and defend against bacterial, fungal and insect pests and pathogens. Induced systemic resistance (ISR) is a systemic immune response that occurs upon root colonization by beneficial microbes. A well-studied model for ISR is the association of specific beneficial strains of Pseudomonas ...
    Assembly of a Custom-made Device to Study Spreading Patterns of Pseudomonas putida Biofilms
    用于恶臭假单胞菌生物膜扩散模式研究的定制设备的组装
    作者:David R. Espeso, Esteban Martínez-García and Víctor de Lorenzo日期:05/20/2019,浏览量:172,Q&A: 0
    [Abstract] Biofilms are bacterial communities in the shape of exopolysaccharide matrix-encased aggregates attached onto interphases able to resist environmental aggressions. The development of bacteria in the shape of biofilms deeply affects the performance of many industrial processes which work with fluidic systems, where bacteria may settle and prosper. ...
    Analysis of Indole-3-acetic Acid (IAA) Production in Klebsiella by LC-MS/MS and the Salkowski Method
    利用液相色谱-串联质谱法和萨尔科夫斯基氏法分析克雷伯菌中吲哚乙酸的产生
    作者:Shraddha Gang, Sheetal Sharma, Meenu Saraf, Martin Buck and Jörg Schumacher日期:05/05/2019,浏览量:292,Q&A: 0
    [Abstract] Many rhizobacteria isolated from plant rhizosphere produce various phytohormones in the form of secondary metabolites, the most common of which is Indole-3-acetic acid (IAA). Here, we detail analytical protocols of IAA detection and quantification, in vitro and in situ, as recently applied to Klebsiella SGM 81, a ...
    Tracking Root Interactions System (TRIS) Experiment and Quality Control
    根相互作用系统的示踪实验和质量控制
    作者:Hassan Massalha, Elisa Korenblum, Orr H. Shapiro and Asaph Aharoni日期:04/20/2019,浏览量:650,Q&A: 0
    [Abstract] Soil organisms are diverse taxonomically and functionally. This ecosystem experiences highly complex networks of interactions, but may also present functionally independent entities. Plant roots, a metabolically active hotspot in the soil, take an essential part in shaping the rhizosphere. Tracking the dynamics of root-microbe interactions at high ...
    Delivering "Chromatic Bacteria" Fluorescent Protein Tags to Proteobacteria Using Conjugation
    利用接合生殖将产色细菌荧光蛋白标签导入变形杆菌
    作者:Rudolf O Schlechter and Mitja NP Remus-Emsermann日期:04/05/2019,浏览量:457,Q&A: 0
    [Abstract] Recently, we published a large and versatile set of plasmids, the chromatic bacteria toolbox, to deliver eight different fluorescent protein genes and four combinations of antibiotic resistance genes to Gram-negative bacteria. Fluorescent tags are important tools for single-cell microbiology, synthetic community studies, biofilm, and host-microbe ...
    Biofilm Assays on Fibrinogen-coated Silicone Catheters and 96-well Polystyrene Plates
    在纤维蛋白原包覆的硅胶导管和96孔聚苯乙烯板上进行生物膜检测
    作者:Cristina Colomer-Winter, José A. Lemos and Ana L. Flores-Mireles日期:03/20/2019,浏览量:667,Q&A: 0
    [Abstract] Biofilm formation is a well-known bacterial strategy that protects cells from hostile environments. During infection, bacteria found in a biofilm community are less sensitive to antibiotics and to the immune response, often allowing them to colonize and persist in the host niche. Not surprisingly, biofilm formation on medical devices, such as ...
    Quantification of Queuosine Modification Levels in tRNA from Human Cells Using APB Gel and Northern Blot
    利用聚乙二醇凝胶和Northern印记法定量分析来自人细胞的tRNA 的Q核苷修饰水平
    作者:Zaneta Matuszek and Tao Pan日期:03/20/2019,浏览量:500,Q&A: 0
    [Abstract] Queuosine (Q) is a hypermodified base in the wobble anticodon position of tRNAs coding for the amino acids Tyr, His, Asn, and Asp. tRNA Q-modification is introduced by a queuine tRNA-ribosyltransferase (TGT) that replaces the guanine base at G34 at these tRNAs with the modified base. tRNA Q-modification is widely distributed among prokaryotic and ...
    QsRNA-seq: A protocol for Generating Libraries for High-throughput Sequencing of Small RNAs
    QsRNA-seq: 小RNA高通量测序文库构建方案
    作者:Alla Fishman and Ayelet T. Lamm日期:03/05/2019,浏览量:755,Q&A: 0
    [Abstract] Small RNAs (sRNAs) are 20-30 nt long non-coding RNA molecules that regulate essentially all cellular processes. Besides being an intensively studied topic in academic research, sRNAs also hold a promise as clinical biomarkers. While the need for expressional profiling of sRNAs is growing, preparation of sRNA libraries for high-throughput ...