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分子生物学

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    Chromatin Affinity Purification (ChAP) from Arabidopsis thaliana Rosette Leaves Using in vivo Biotinylation System
    使用体内生物素化系统进行拟南芥莲座叶染色质亲和纯化(ChAP)
    作者:Weronika Sura and Piotr A. Ziolkowski日期:01/05/2018,浏览量:117,Q&A: 0
    Chromatin Affinity Purification (ChAP) is widely used to study chromatin architecture and protein complexes interacting with DNA. Here we present an efficient method for ChAP from Arabidopsis thaliana rosette leaves, in which in vivo ...
    Targeted Genome Editing of Virulent Phages Using CRISPR-Cas9
    使用CRISPR-Cas9对烈性噬菌体进行靶向基因组编辑
    作者:Marie-Laurence Lemay, Ariane C. Renaud, Geneviève M. Rousseau and Sylvain Moineau日期:01/05/2018,浏览量:219,Q&A: 0
    This protocol describes a straightforward method to generate specific mutations in the genome of strictly lytic phages. Briefly, a targeting CRISPR-Cas9 system and a repair template suited for homologous recombination are provided inside a bacterial ...
    Rolling Circle Amplification to Screen Yam Germplasm for Badnavirus Infections and to Amplify and Characterise Novel Badnavirus Genomes
    滚换扩增筛选山药Badnavirus感染种质并扩增鉴定新型Badnavirus基因组
    作者:Moritz Bömer, Aliyu A. Turaki, Ajith I. Rathnayake, Gonçalo Silva, P. Lava Kumar and Susan E. Seal日期:01/05/2018,浏览量:115,Q&A: 0
    Since the first discovery of badnaviruses (family Caulimoviridae, genus Badnavirus) in yam (Dioscorea spp.) germplasm in the 1970s (Harrison and Roberts, 1973), several hundred partial badnavirus reverse transcriptase ...
    Chromatin Affinity Purification (ChAP) from Arabidopsis thaliana Rosette Leaves Using in vivo Biotinylation System
    使用体内生物素化系统进行拟南芥莲座叶染色质亲和纯化(ChAP)
    作者:Weronika Sura and Piotr A. Ziolkowski日期:01/05/2018,浏览量:117,Q&A: 0
    [Abstract] Chromatin Affinity Purification (ChAP) is widely used to study chromatin architecture and protein complexes interacting with DNA. Here we present an efficient method for ChAP from Arabidopsis thaliana rosette leaves, in which in vivo biotinylation system is used. The chromatin is digested by Micrococcal Nuclease (MNase), hence ...
    Targeted Genome Editing of Virulent Phages Using CRISPR-Cas9
    使用CRISPR-Cas9对烈性噬菌体进行靶向基因组编辑
    作者:Marie-Laurence Lemay, Ariane C. Renaud, Geneviève M. Rousseau and Sylvain Moineau日期:01/05/2018,浏览量:219,Q&A: 0
    [Abstract] This protocol describes a straightforward method to generate specific mutations in the genome of strictly lytic phages. Briefly, a targeting CRISPR-Cas9 system and a repair template suited for homologous recombination are provided inside a bacterial host, here the Gram-positive model Lactococcus lactis MG1363. The CRISPR-Cas9 system is ...
    Rolling Circle Amplification to Screen Yam Germplasm for Badnavirus Infections and to Amplify and Characterise Novel Badnavirus Genomes
    滚换扩增筛选山药Badnavirus感染种质并扩增鉴定新型Badnavirus基因组
    作者:Moritz Bömer, Aliyu A. Turaki, Ajith I. Rathnayake, Gonçalo Silva, P. Lava Kumar and Susan E. Seal日期:01/05/2018,浏览量:115,Q&A: 0
    [Abstract] Since the first discovery of badnaviruses (family Caulimoviridae, genus Badnavirus) in yam (Dioscorea spp.) germplasm in the 1970s (Harrison and Roberts, 1973), several hundred partial badnavirus reverse transcriptase (RT)-ribonuclease H (RNaseH) sequences have been characterised (Kenyon et al., 2008; Bousalem ...
    Genotyping-free Selection of Double Allelic Gene Edited Medaka Using Two Different Fluorescent Proteins
    使用两种不同的荧光蛋白进行双等位基因编辑青鳉的无基因分型选择
    作者:Yu Murakami, Satoshi Ansai, Akari Yonemura and Masato Kinoshita日期:12/20/2017,浏览量:293,Q&A: 0
    [Abstract] This protocol describes a simple genotyping using two different colors of fluorescent protein genes inserted at the target locus. This method makes it possible to determine the genotype of each individual simply by observing the fluorescence later than F1 generation.
    MicroScale Thermophoresis as a Tool to Study Protein-peptide Interactions in the Context of Large Eukaryotic Protein Complexes
    微量热泳动法研究大真核生物蛋白复合物中蛋白质 - 肽相互作用
    作者:Maximilian G. Plach, Klaus Grasser and Thomas Schubert日期:12/05/2017,浏览量:564,Q&A: 0
    [Abstract] Protein-peptide interactions are part of many physiological processes, for example, epigenetics where peptide regions of histone complexes are crucial for regulation of chromatin structure. Short peptides are often also used as alternatives to small molecule drugs to target protein complexes. Studying the interactions between proteins and peptides ...
    Low-input Capture-C: A Chromosome Conformation Capture Assay to Analyze Chromatin Architecture in Small Numbers of Cells
    低样品量Capture-C:采用染色体构象采集分析法分析少量细胞中的染色质结构
    作者:A. Marieke Oudelaar, Damien J. Downes, James O.J. Davies and Jim R. Hughes日期:12/05/2017,浏览量:901,Q&A: 0
    [Abstract] Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This protocol describes two new low-input Capture-C approaches that can generate high-quality 3C interaction profiles from 10,000-20,000 cells, depending on the ...
    Genome Editing in Diatoms Using CRISPR-Cas to Induce Precise Bi-allelic Deletions
    通过CRISPR-Cas精确诱导双等位基因缺失以对硅藻进行基因组编辑
    作者:Amanda Hopes, Vladimir Nekrasov, Nigel Belshaw, Irina Grouneva, Sophien Kamoun and Thomas Mock日期:12/05/2017,浏览量:486,Q&A: 0
    [Abstract] Genome editing in diatoms has recently been established for the model species Phaeodactylum tricornutum and Thalassiosira pseudonana. The present protocol, although developed for T. pseudonana, can be modified to edit any diatom genome as we utilize the flexible, modular Golden Gate cloning system. The main steps include ...
    Stationary-phase Mutagenesis Soft-agar Overlay Assays in Bacillus subtilis
    枯草芽孢杆菌的软琼脂糖覆盖固相诱变
    [Abstract] Elucidating how a population of non-growing bacteria generates mutations improves our understanding of phenomena like antibiotic resistance, bacterial pathogenesis, genetic diversity and evolution. To evaluate mutations that occur in nutritionally stressed non-growing bacteria, we have employed the strain B. subtilis YB955, which measures ...
    [Bio101] RNA Extraction from Wistar Rat Cochlea for qRT-PCR
    从Wistar大鼠耳蜗中提取RNA用于qRT-PCR分析
    作者:Janaína Cândida Rodrigues and Rubens Vuono de Brito Neto日期:12/05/2017,浏览量:245,Q&A: 0
    [Abstract] Otology research has developed considerably in recent years and molecular analysis is crucial to identify metabolic pathways and therapeutic targets. However, the structure of the cochlea limits the amount of cell mass, and special care is required for RNA extraction. Studies applying this technique to the cochlea are scarce in the literature, and ...
    Design and Direct Assembly of Synthesized Uracil-containing Non-clonal DNA Fragments into Vectors by USERTM Cloning
    设计并通过USERTM克隆直接将合成的含尿嘧啶非克隆DNA片段装配到载体中
    [Abstract] This protocol describes how to order and directly assemble uracil-containing non-clonal DNA fragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal of making synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol is highly efficient and would be compatible ...