分子生物学

分类

    现刊
    A Molecular Cloning and Sanger Sequencing-based Protocol for Detecting Site-specific DNA Methylation
    一种基于分子克隆和Sanger测序的位点特异性DNA甲基化检测方案
    作者:Wei Guo, Anthony Cannon and Damon Lisch日期:05/05/2022,浏览量:426,Q&A: 0

    DNA methylation is a conserved chemical modification, by which methyl groups are added to the cytosine of DNA molecules. Methylation can influence gene expression without changing the sequence of a particular gene. This epigenetic effect is an

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    A Molecular Cloning and Sanger Sequencing-based Protocol for Detecting Site-specific DNA Methylation
    一种基于分子克隆和Sanger测序的位点特异性DNA甲基化检测方案
    作者:Wei Guo, Anthony Cannon and Damon Lisch日期:05/05/2022,浏览量:426,Q&A: 0
    [Abstract]

    DNA methylation is a conserved chemical modification, by which methyl groups are added to the cytosine of DNA molecules. Methylation can influence gene expression without changing the sequence of a particular gene. This epigenetic effect is an intriguing phenomenon that has puzzled biologists for years. By probing the temporal and spatial patterns

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    An Improved EMSA-based Method to Prioritize Candidate cis-REs for Further Functional Validation
    一种改进的基于EMSA的方法来优先考虑候选的顺式RE,以进一步验证其功能
    作者:Ting Wu and Gang Li日期:04/20/2022,浏览量:439,Q&A: 0
    [Abstract]

    Cells are the complex product of gene expression programs that involve the coordinated transcription of thousands of genes controlled by cis-regulatory elements (cis-REs). Therefore, identification of cis-REs is the key to decipher the mechanisms underlying the regulation of gene expression. Here, we describe a simple and time-effective protocol

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    circFL-seq, A Full-length circRNA Sequencing Method
    circFL-seq,一种全长circRNA的测序方法
    作者:Zelin Liu and Ence Yang日期:04/20/2022,浏览量:756,Q&A: 1
    [Abstract]

    Due to overlapping sequences with linear cognates, identifying internal sequences of circular RNA (circRNA) remains a challenge. Recently, we have developed a full-length circRNA sequencing method (circFL-seq) and computational pipeline, to profile ordinary and fusion circRNA at the isoform level. Compared to short-read RNA-seq, rolling circular

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    An Optimized Tat/Rev Induced Limiting Dilution Assay for the Characterization of HIV-1 Latent Reservoirs
    一种优化的 Tat/Rev 诱导的限制稀释测定表征 HIV-1 潜伏储库
    [Abstract]

    The administration of antiretroviral therapy (ART) leads to a rapid reduction in plasma viral load in HIV-1 seropositive subjects. However, when ART is suspended, the virus rebounds due to the presence of a latent viral reservoir. Several techniques have been developed to characterize this latent viral reservoir. Of the various assay formats

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    Application of Cadaverine to Inhibit Biotin Biosynthesis in Plants
    尸胺在抑制植物生物素合成中的应用
    作者:Nicole M. Gibbs, Shih-Heng Su and Patrick H. Masson日期:04/20/2022,浏览量:382,Q&A: 0
    [Abstract]

    Biotin is an essential vitamin in plants. However, characterization of biotin deficiency has been limited by embryo lethality in mutants, which can only be rescued by supplementation of biotin. Here, we describe a protocol to characterize biotin deficiency in Arabidopsis thaliana through application of the polyamine cadaverine. Cadaverine

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    RNA Interference Method for Gene Function Analysis in the Japanese Rhinoceros Beetle Trypoxylus dichotomus
    日本犀牛甲虫Trypoxylus dichotomus基因功能分析的RNA干扰方法
    作者:Kazuki Sakura, Shinichi Morita and Teruyuki Niimi日期:04/20/2022,浏览量:693,Q&A: 0
    [Abstract]

    In the Japanese rhinoceros beetle Trypoxylus dichotomus, various candidate genes required for a specific phenotype of interest are listed by next-generation sequencing analysis. Their functions were investigated using RNA interference (RNAi) method, the only gene function analysis tool for T. dichotomus developed so far. The summarized procedure

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    Plasmid and Sequencing Library Preparation for CRISPRi Barcoded Expression Reporter Sequencing (CiBER-seq) in Saccharomyces cerevisiae
    用于酿酒酵母中 CRISPRi 条形码表达报告基因测序 (CiBER-seq) 的质粒和测序文库制备
    作者:Ryan Y Muller, Zuriah A Meacham and Nicholas T Ingolia日期:04/05/2022,浏览量:668,Q&A: 0
    [Abstract]

    Genetic networks regulate nearly all biological processes, including cellular differentiation, homeostasis, and immune responses. Determining the precise role of each gene within a regulatory network can explain its overall, integrated function, and pinpoint mechanisms underlying misregulation in disease states. Transcriptional reporter assays are

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    Conditional Human BRD4 Knock-In Transgenic Mouse Genotyping and Protein Isoform Detection
    条件性人 BRD4 敲入转基因小鼠基因分型和蛋白质异构体检测
    作者:Michael Paul Lewis, Shwu-Yuan Wu and Cheng-Ming Chiang日期:04/05/2022,浏览量:409,Q&A: 0
    [Abstract]

    Bromodomain-containing protein 4 (BRD4) is an acetyl-lysine reader protein and transcriptional regulator implicated in chromatin dynamics and cancer development. Several BRD4 isoforms have been detected in humans with the long isoform (BRD4-L, aa 1-1,362) playing a tumor-suppressive role and a major short isoform (BRD4-S, aa 1-722) having

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    A Rapid FRET Real-Time PCR Protocol for Simultaneous Quantitative Detection and Discrimination of Human Plasmodium Parasites
    一种同时定量检测和鉴别人类疟原虫的快速FRET实时PCR方法
    [Abstract]

    Malaria is the most important parasitic disease worldwide, and accurate diagnosis and treatment without delay are essential for reducing morbidity and mortality, especially in P. falciparum malaria. Real-time PCR is highly sensitive and highly specific, therefore an excellent diagnostic tool for laboratory detection and species-specific

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    Single-cell Damagenome Profiling by Linear Copying and Splitting based Whole Genome Amplification (LCS-WGA)
    基于线性复制和分裂的全基因组扩增法(LCS-WGA)分析单细胞损伤组
    作者:Yichi Niu, Qiangyuan Zhu and Chenghang Zong日期:03/20/2022,浏览量:483,Q&A: 0
    [Abstract]

    Spontaneous DNA damage frequently occurs on the human genome, and it could alter gene expression by inducing mutagenesis or epigenetic changes. Therefore, it is highly desired to profile DNA damage distribution on the human genome and identify the genes that are prone to DNA damage. Here, we present a novel single-cell whole-genome amplification

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