分子生物学

分类

    现刊
    A Radioactive-free Kinase Inhibitor Discovery Assay Against the Trypanosoma brucei Glycogen Synthase Kinase-3 short (TbGSK-3s)
    一种无放射性布氏锥虫糖原合成酶激酶-3抑制剂探究实验
    作者:Antonia Efstathiou and Despina Smirlis日期:01/20/2020,浏览量:83,Q&A: 0
    The identification of small molecules possessing inhibitory activity in vitro, against a given target kinase, is the first step in the drug discovery process. Herein, we describe a non radioactive protocol using luciferase-based ATP assay ...
    Measurement of the Promoter Activity in Escherichia coli by Using a Luciferase Reporter
    利用荧光素酶报告系统检测大肠杆菌中启动子活性
    作者:Yuki Yamanaka, Hiroki Watanabe, Erika Yamauchi, Yukari Miyake and Kaneyoshi Yamamoto日期:01/20/2020,浏览量:95,Q&A: 0
    The reporter system is widely used technique for measuring promoter activity in bacterial cells. Until now, a number of reporter system have been developed, but the bioluminescent reporter constructed from the bacterial luciferase genes is one of ...
    Ribonucleoprotein Immunoprecipitation (RIP) Analysis
    核糖核蛋白免疫沉淀分析
    作者:Jennifer L. Martindale, Myriam Gorospe and Maria L. Idda日期:01/20/2020,浏览量:217,Q&A: 0
    RNAs and RNA-binding proteins (RBPs) can interact dynamically in ribonucleoprotein (RNP) complexes that play important roles in controlling gene expression programs. One of the powerful ways to investigate changes in the association of RNAs with an ...
    A Radioactive-free Kinase Inhibitor Discovery Assay Against the Trypanosoma brucei Glycogen Synthase Kinase-3 short (TbGSK-3s)
    一种无放射性布氏锥虫糖原合成酶激酶-3抑制剂探究实验
    作者:Antonia Efstathiou and Despina Smirlis日期:01/20/2020,浏览量:83,Q&A: 0
    [Abstract] The identification of small molecules possessing inhibitory activity in vitro, against a given target kinase, is the first step in the drug discovery process. Herein, we describe a non radioactive protocol using luciferase-based ATP assay for the identification of inhibitors for the short isoform of the Trypanosoma brucei’s ...
    Measurement of the Promoter Activity in Escherichia coli by Using a Luciferase Reporter
    利用荧光素酶报告系统检测大肠杆菌中启动子活性
    作者:Yuki Yamanaka, Hiroki Watanabe, Erika Yamauchi, Yukari Miyake and Kaneyoshi Yamamoto日期:01/20/2020,浏览量:95,Q&A: 0
    [Abstract] The reporter system is widely used technique for measuring promoter activity in bacterial cells. Until now, a number of reporter system have been developed, but the bioluminescent reporter constructed from the bacterial luciferase genes is one of the useful systems for measuring in vivo dynamics of gene expression. The introduced ...
    Ribonucleoprotein Immunoprecipitation (RIP) Analysis
    核糖核蛋白免疫沉淀分析
    作者:Jennifer L. Martindale, Myriam Gorospe and Maria L. Idda日期:01/20/2020,浏览量:217,Q&A: 0
    [Abstract] RNAs and RNA-binding proteins (RBPs) can interact dynamically in ribonucleoprotein (RNP) complexes that play important roles in controlling gene expression programs. One of the powerful ways to investigate changes in the association of RNAs with an RBP of interest is by immunoprecipitation (IP) analysis of native RNPs. RIP (RNP ...
    RNA Interactome Identification via RNA-BioID in Mouse Embryonic Fibroblasts
    利用 RNA-BioID鉴定小鼠胚胎成纤维细胞中RNA相互作用体
    作者:Joyita Mukherjee, Mirita Franz-Wachtel, Boris Maček and Ralf-Peter Jansen日期:01/05/2020,浏览量:228,Q&A: 0
    [Abstract] Cytoplasmic localization of mRNAs is common to all organisms and serves the spatial expression of genes. Cis-acting RNA signals (mostly found in the mRNA’s 3'-UTR), called zipcodes recruit trans-acting RNA-binding proteins that facilitate the localization of the mRNA. UV-cross-linking or affinity purification has been applied to identify such ...
    Laser Capture Micro-dissection (LCM) of Neonatal Mouse Forebrain for RNA Isolation
    新生小鼠前脑激光捕获显微切割(LCM)分离RNA
    作者:Achira Roy, Mei Deng, Kimberly A. Aldinger, Ian A. GLass and Kathleen J. Millen日期:01/05/2020,浏览量:287,Q&A: 0
    [Abstract] Precise and reproducible isolation of desired cell types or layers from heterogeneous tissues is crucial to analyze specific gene profiles and molecular interactions in vivo. Forebrain is the core site of higher functions, like cognition and memory consolidation. It is composed of heterogeneous and distinct cell types, interconnected to ...
    Unbiased and Tailored CRISPR/Cas gRNA Libraries by Synthesizing Covalently-closed-circular (3Cs) DNA
    通过合成共价闭合环状(3Cs)DNA构建无偏和专用CRISPR / Cas gRNA文库
    作者:Martin Wegner, Koraljka Husnjak and Manuel Kaulich日期:01/05/2020,浏览量:514,Q&A: 0
    [Abstract] Simplicity, efficiency and versatility of the CRISPR/Cas system greatly contributed to its rapid use in a broad range of fields. Applications of unbiased CRISPR/Cas screenings are increasing and thus there is a growing need for unbiased and tailored CRISPR/Cas gRNA libraries. Conventional methods for gRNA library generation apply PCR and cloning ...
    High-throughput Site-directed Scanning Mutagenesis Using a Two-fragment PCR Approach
    利用双片段PCR法进行高通量定点扫描突变
    作者:Franziska M. Heydenreich, Tamara Miljuš, Dalibor Milić and Dmitry B. Veprintsev日期:01/05/2020,浏览量:280,Q&A: 0
    [Abstract] Site-directed scanning mutagenesis is a useful tool applied in studying protein function and designing proteins with new properties, such as increased stability or enzymatic activity. Creating a systematic library of hundreds of site-directed mutants is still a demanding and expensive task. The established protocols for making such libraries ...
    A Yeast Chromatin-enriched Fractions Purification Approach, yChEFs, from Saccharomyces cerevisiae
    酿酒酵母的染色质富集组分的纯化方法—yChEFs
    [Abstract] We have adapted a previous procedure and improved an approach that we named yChEFs (yeast Chromatin Enriched Fractions) for purifying chromatin fractions. This methodology allows the easy, reproducible and scalable recovery of proteins associated with chromatin. By using yChEFs, we bypass subcellular fractionation requirements involved when using ...
    Protocol for Ribosome Profiling in Bacteria
    细菌核糖体印迹测序方法
    作者:Fuad Mohammad and Allen R. Buskirk日期:12/20/2019,浏览量:628,Q&A: 0
    [Abstract] Ribosome profiling provides information on the position of ribosomes on mRNA on a genomic scale. Although this information is often used to detect changes in gene expression under different conditions, it also has great potential for yielding insight into the mechanism and regulation of protein synthesis itself. First developed in yeast, ribosome ...
    Semi-quantitative Determination of Protein Expression Using Immunohistochemistry Staining and Analysis: An Integrated Protocol
    一种综合的实验方法:利用免疫组织化学染色和分析进行蛋白表达的半定量测定
    作者:Alexandra R Crowe and Wei Yue日期:12/20/2019,浏览量:722,Q&A: 0
    [Abstract] Semi-quantitative IHC is a powerful method for investigating protein expression and localization within tissues. The semi-quantitative immunohistochemistry (IHC) involves using software such as free software ImageJ Fiji to conduct deconvolution and downstream analysis. Currently, there is lack of an integrated protocol that includes a detailed ...