植物

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    现刊
    An Adjustable Protocol to Analyze Chemical Profiles of Non-sterile Rhizosphere Soil
    一种可调整的用于分析研究非无菌根际土壤化学特性的方法
    作者:Alex Williams, Jurriaan Ton and Pierre Pétriacq日期:05/20/2019,浏览量:744,Q&A: 0
    [Abstract] The analysis of chemical diversity in non-sterile rhizosphere soil has been a pressing methodological challenge for years. Rhizosphere-enriched chemicals (i.e., rhizochemicals) include root exudation chemicals, (microbial) breakdown products thereof, and de novo produced metabolites by rhizosphere-inhabiting microbes, all of ...
    An Improved Bioassay to Study Arabidopsis Induced Systemic Resistance (ISR) Against Bacterial Pathogens and Insect Pests
    一种的可用于检测拟南芥中细菌性病原体和害虫诱导的系统抗性的改进生物学方法
    作者:Nicolás M. Cecchini, Yi Song, Suruchi Roychoudhry, Jean T. Greenberg and Cara H. Haney日期:05/20/2019,浏览量:993,Q&A: 0
    [Abstract] The plant immune system is essential for plants to perceive and defend against bacterial, fungal and insect pests and pathogens. Induced systemic resistance (ISR) is a systemic immune response that occurs upon root colonization by beneficial microbes. A well-studied model for ISR is the association of specific beneficial strains of Pseudomonas ...
    Fabrication and Use of the Dual-Flow-RootChip for the Imaging of Arabidopsis Roots in Asymmetric Microenvironments
    制造和使用dual-flow-RootChip在不对称微环境下进行拟南芥根组织成像
    [Abstract] This protocol provides a detailed description of how to fabricate and use the dual-flow-RootChip (dfRootChip), a novel microfluidic platform for investigating root nutrition, root-microbe interactions and signaling and development in controlled asymmetric conditions. The dfRootChip was developed primarily to investigate how plants roots interact ...
    Evaluation of Root pH Change Through Gel Containing pH-sensitive Indicator Bromocresol Purple
    通过含有pH敏感指示剂溴甲酚紫的凝胶评估根部pH值的变化
    [Abstract] The Rapid Alkalinization Factor (RALF) is a plant hormone peptide that inhibits proton transport causing alkalinization of the extracellular media. To detect the alkalinization response elicited by RALF peptides in root cells, Arabidopsis seedlings are carefully transferred to a gel containing the pH-sensitive indicator bromocresol ...
    Determination of the Effects of Local and Systemic Iron Excess on Lateral Root Initiation in Arabidopsis thaliana
    局部和系统性铁过量对拟南芥侧根起始影响的测定
    作者:Guangjie Li, Lin Zhang and Weiming Shi日期:07/05/2017,浏览量:3141,Q&A: 0
    [Abstract] Root system architecture depends on nutrient availability. A symptom of iron (Fe) toxicity in plants is stunted root growth, yet little is known about the effects of excess Fe on lateral root (LR) development. To better understand how nutrient signals are integrated into root developmental programs, we investigated the morphological response of ...
    Hydrogen Peroxide Measurement in Arabidopsis Root Tissue Using Amplex Red
    Amplex Red荧光法测定拟南芥根组织中的过氧化氢酶
    作者:Tzvetina Brumbarova, Cham Thi Tuyet Le and Petra Bauer日期:11/05/2016,浏览量:7872,Q&A: 0
    [Abstract] This protocol describes the measurement of hydrogen peroxide (H2O2) content in Arabidopsis root tissue by using the Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit. When root tissue is disrupted and resuspended in phosphate buffer, H2O2 is released from the cells. The obtained root ...
    A Live-imaging, Heat Shock-inducible System to Measure Aux/IAA Degradation Rates in Planta
    活体成像的热激诱导系统测定植株的Aux/IAA降解率
    作者:Jessica M. Guseman, Jennifer L. Nemhauser and Britney L. Moss日期:08/05/2016,浏览量:5766,Q&A: 0
    [Abstract] An emerging theme in biology is the importance of cellular signaling dynamics. In addition to monitoring changes in absolute abundance of signaling molecules, many signal transduction pathways are sensitive to changes in temporal properties of signaling components (Purvis and Lahav, 2013). The phytohormone auxin regulates myriad processes in plant ...
    Root-knot Nematode Penetration and Sclareol Nematicidal Activity Assays
    根结线虫的渗透和香紫苏醇杀线虫的活性分析
    作者:Taketo Fujimoto, Takayuki Mizukubo, Hiroshi Abe and Shigemi Seo日期:06/20/2016,浏览量:5310,Q&A: 0
    [Abstract] Plant parasitic nematodes parasitize roots and/or stems of various plants thereby inhibiting absorption of nutrients and moisture. In particular, root-knot nematodes (RKN) are a group of the most devastating pests. Various techniques, such as soil sterilization, cultivation of resistant crops, and chemical application, have been developed to ...
    Rhizosphere Acidification Assay
    根际酸化试验
    作者:Gaston A. Pizzio, Kamesh Regmi and Roberto Gaxiola日期:12/05/2015,浏览量:4774,Q&A: 0
    [Abstract] Plant survival depends on the ability of root systems to establish themselves in locations where water and nutrients are available for uptake and translocation (Hawes et al., 2003). Rhizosphere influences crop productivity by mediating efficient nutrient transformation, acquisition, and use (Shen et al., 2013). Rhizosphere ...
    Infection Assay of Cyst Nematodes on Arabidopsis Roots
    拟南芥根部的胞囊线虫感染实验
    作者:Holger Bohlmann and Krzysztof Wieczorek日期:09/20/2015,浏览量:6716,Q&A: 0
    [Abstract] Plant parasitic nematodes are devastating pests on many crops. Juveniles (J2) of cyst nematodes invade the roots to induce a syncytium. This feeding site is their only source of nutrients. Male nematodes leave the roots after the fourth molt to mate with females. The females stay attached to their syncytia throughout their life and produce ...