Tandem Tag Assay Optimized for Semi-automated in vivo Autophagic Activity Measurement in Arabidopsis thaliana roots
    作者:Adrian N. Dauphinee, Jonas A. Ohlsson and Elena A. Minina日期:03/05/2020,浏览量:705,Q&A: 0
    [Abstract] Autophagy is the main catabolic process in eukaryotes and plays a key role in cell homeostasis. In vivo measurement of autophagic activity (flux) is a powerful tool for investigating the role of the pathway in organism development and stress responses. Here we describe a significant optimization of the tandem tag assay for detection of ...
    An Adjustable Protocol to Analyze Chemical Profiles of Non-sterile Rhizosphere Soil
    作者:Alex Williams, Jurriaan Ton and Pierre Pétriacq日期:05/20/2019,浏览量:2251,Q&A: 0
    [Abstract] The analysis of chemical diversity in non-sterile rhizosphere soil has been a pressing methodological challenge for years. Rhizosphere-enriched chemicals (i.e., rhizochemicals) include root exudation chemicals, (microbial) breakdown products thereof, and de novo produced metabolites by rhizosphere-inhabiting microbes, all of ...
    An Improved Bioassay to Study Arabidopsis Induced Systemic Resistance (ISR) Against Bacterial Pathogens and Insect Pests
    作者:Nicolás M. Cecchini, Yi Song, Suruchi Roychoudhry, Jean T. Greenberg and Cara H. Haney日期:05/20/2019,浏览量:2669,Q&A: 0
    [Abstract] The plant immune system is essential for plants to perceive and defend against bacterial, fungal and insect pests and pathogens. Induced systemic resistance (ISR) is a systemic immune response that occurs upon root colonization by beneficial microbes. A well-studied model for ISR is the association of specific beneficial strains of Pseudomonas ...
    Tracking Root Interactions System (TRIS) Experiment and Quality Control
    作者:Hassan Massalha, Elisa Korenblum, Orr H. Shapiro and Asaph Aharoni日期:04/20/2019,浏览量:2489,Q&A: 0
    [Abstract] Soil organisms are diverse taxonomically and functionally. This ecosystem experiences highly complex networks of interactions, but may also present functionally independent entities. Plant roots, a metabolically active hotspot in the soil, take an essential part in shaping the rhizosphere. Tracking the dynamics of root-microbe interactions at high ...
    Fabrication and Use of the Dual-Flow-RootChip for the Imaging of Arabidopsis Roots in Asymmetric Microenvironments
    [Abstract] This protocol provides a detailed description of how to fabricate and use the dual-flow-RootChip (dfRootChip), a novel microfluidic platform for investigating root nutrition, root-microbe interactions and signaling and development in controlled asymmetric conditions. The dfRootChip was developed primarily to investigate how plants roots interact ...
    Evaluation of Root pH Change Through Gel Containing pH-sensitive Indicator Bromocresol Purple
    [Abstract] The Rapid Alkalinization Factor (RALF) is a plant hormone peptide that inhibits proton transport causing alkalinization of the extracellular media. To detect the alkalinization response elicited by RALF peptides in root cells, Arabidopsis seedlings are carefully transferred to a gel containing the pH-sensitive indicator bromocresol ...
    Electron Tomography to Study the Three-dimensional Structure of Plasmodesmata in Plant Tissues–from High Pressure Freezing Preparation to Ultrathin Section Collection
    采用电子断层摄影术研究植物组织中的胞间连丝的三维结构 — 从高压冷冻制备到超薄切片采集
    作者:William J. Nicolas, Emmanuelle Bayer and Lysiane Brocard日期:01/05/2018,浏览量:4911,Q&A: 0
    [Abstract] Plasmodesmata (PD) are nanometric (~20 nm wide) membrane lined pores encased in the cell walls of the adjacent plant cells. They allow the cells to exchange all types of molecules ranging from nutrients like sugar, hormones, to RNAs and various proteins. Unfortunately, they are also hijacked by phyto-viruses, enabling them to spread from ...
    Ensifer-mediated Arabidopsis thaliana Root Transformation (E-ART): A Protocol to Analyse the Factors that Support Ensifer-mediated Transformation (EMT) of Plant Cells
    剑菌介导的拟南芥根系转化(E-ART): 分析利于剑菌介导植物细胞转化(EMT)因素的实验方案
    作者:Dheeraj Singh Rathore, Fiona M. Doohan and Ewen Mullins日期:10/05/2017,浏览量:6097,Q&A: 0
    [Abstract] Ensifer adhaerens OV14, a soil borne alpha-proteobacteria of the Rhizobiaceae family, fortifies the novel plant transformation technology platform termed ‘Ensifer-mediated transformation’ (EMT). EMT can stably transform both monocot and dicot species, and the host range of EMT is continuously expanding across a diverse range of ...
    Assessing Plant Tolerance to Acute Heat Stress
    作者:Minsoo Kim, Fionn McLoughlin, Eman Basha and Elizabeth Vierling日期:07/20/2017,浏览量:6793,Q&A: 1
    [Abstract] It is well-established that plants are able to acclimate to temperatures above or below the optimal temperature for their growth. Here, we provide protocols for assays that can be used quantitatively or qualitatively to assess the relative ability of plants to acquire tolerance to high temperature stress. The hypocotyl elongation assay described ...
    Determination of the Effects of Local and Systemic Iron Excess on Lateral Root Initiation in Arabidopsis thaliana
    作者:Guangjie Li, Lin Zhang and Weiming Shi日期:07/05/2017,浏览量:4541,Q&A: 0
    [Abstract] Root system architecture depends on nutrient availability. A symptom of iron (Fe) toxicity in plants is stunted root growth, yet little is known about the effects of excess Fe on lateral root (LR) development. To better understand how nutrient signals are integrated into root developmental programs, we investigated the morphological response of ...