Visualizing Hypoxia in a Murine Model of Candida albicans Infection Using in vivo Biofluorencence
    作者:José Pedro Lopes and Constantin F. Urban日期:08/05/2019,浏览量:272,Q&A: 0
    [Abstract] Candida albicans is a leading human fungal pathogen that uses several metabolic adaptations to escape immune cells and causes systemic disease. Here, we describe a protocol for measuring one of these adaptations, the ability to thrive in hypoxic niches. Hypoxia was generated after successful subdermal infection with C. albicans ...
    Quantification of Prostaglandin E2 Concentration in Interstitial Fluid from the Hypothalamic Region of Free-moving Mice
    作者:Yoshinobu Nakamura, Takatoshi Sakaguchi, Ikumi Tamai and Takeo Nakanishi日期:08/05/2019,浏览量:209,Q&A: 0
    [Abstract] Prostaglandin E2 (PGE2) is a well-established chemical mediator for the generation of the fever at the hypothalamus of the brain. PGE2 mediates fever generation via PGE receptor 3 (i.e., EP3) on neurons in the preoptic area. The role of PGE2 has been analyzed by measuring PGE2 ...
    Looking through Brains with Fast Passive CLARITY: Zebrafish, Rodents, Non-human Primates and Humans
    利用 Fast Passive CLARITY技术研究斑马鱼、啮齿动物、灵长类动物和人类的大脑
    作者:Farzad Mortazavi, Alexander J. Stankiewicz and Irina V. Zhdanova日期:08/05/2019,浏览量:235,Q&A: 0
    [Abstract] Recently developed CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ-hybridization-compatible Tis-sue-hYdrogel) technique renders the tissue transparent by removing lipids in the tissue, while preserving and stabilizing the cellular and subcellular structures. This provides effective penetration of diverse ...
    Precision Technique for Splenectomy Limits Mouse Stress Responses for Accurate and Realistic Measurements for Investigating Inflammation and Immunity
    作者:Shengwen Calvin Li, Anthony D. Rangel and Mustafa H. Kabeer日期:08/05/2019,浏览量:229,Q&A: 0
    [Abstract] Splenectomy in an animal model requires a standardized technique utilizing best practice to avoid variability which can result in adverse impact to the animal resulting in flawed physiologic responses simply due to technique rather than to the studied variables. In the case of the spleen, often investigators are analyzing the animal immune or ...
    Isolation and Long-term Cultivation of Mouse Alveolar Macrophages
    作者:Clara Jana-Lui Busch, Jérémy Favret, Laufey Geirsdóttir, Kaaweh Molawi and Michael H. Sieweke日期:07/20/2019,浏览量:771,Q&A: 0
    [Abstract] Alveolar macrophages (AM) are tissue-resident macrophages that colonize the lung around birth and can self-maintain long-term in an adult organism without contribution of monocytes. AM are located in the pulmonary alveoli and can be harvested by washing the lungs using the method of bronchoalveolar lavage (BAL). Here, we compared different ...
    Isolation and Culture of Single Myofiber and Immunostaining of Satellite Cells from Adult C57BL/6J Mice
    作者:Shujie Chen, Hongrong Ding, Xiangping Yao and Liwei Xie日期:07/20/2019,浏览量:532,Q&A: 0
    [Abstract] Myofiber isolation followed with ex vivo culture could recapitulate and visualize satellite cells (SCs) activation, proliferation, and differentiation. This approach could be taken to understand the physiology of satellite cells and the molecular mechanism of regulatory factors, in terms of the involvement of intrinsic factors over SCs ...
    Axon-seq for in Depth Analysis of the RNA Content of Neuronal Processes
    神经元突起RNA含量的Axon-seq 深入分析
    作者:Jik Nijssen, Julio Aguila and Eva Hedlund日期:07/20/2019,浏览量:609,Q&A: 0
    [Abstract] Neuronal processes have an RNA composition that is distinct from the cell body. Therefore, to fully understand neuronal biology in health and disease we need to study both somas, dendrites and axons. Here we describe a detailed protocol of a newly refined method, Axon-seq, for RNA sequencing of axons (and dendrites) grown in isolation using single ...
    Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina
    作者:Sowmya Bekshe Lokappa, M. Carter Cornwall and Jeannie Chen日期:07/20/2019,浏览量:1141,Q&A: 0
    [Abstract] Rhodopsin is a G-protein coupled receptor (GPCR) that mediates vision under dim light. Upon light exposure, rhodopsin is phosphorylated at multiple serine and threonine sites at its carboxyl-terminus by rhodopsin kinase (GRK1). This, in turn, reduces its ability to activate the visual G-protein transducin. Binding of light-activated, ...
    Protocol for Measuring Compulsive-like Feeding Behavior in Mice
    作者:Marta Fructuoso, Jose Espinosa-Carrasco, Ionas Erb, Cedric Notredame and Mara Dierssen日期:07/20/2019,浏览量:398,Q&A: 0
    [Abstract] Obesity is an important health problem with a strong environmental component that is acquiring pandemic proportion. The high availability of caloric dense foods promotes overeating potentially causing obesity. Animal models are key to validate novel therapeutic strategies, but researchers must carefully select the appropriate model to draw the ...
    Lipid-exchange Rate Assay for Lipid Droplet Fusion in Live Cells
    作者:Jia Wang, Boon Tin Chua, Peng Li and Feng-Jung Chen日期:07/20/2019,浏览量:476,Q&A: 0
    [Abstract] Lipid droplets (LDs) are central organelles in maintaining lipid homeostasis. Defective LD growth often results in the development of metabolic disorders. LD fusion and growth mediated by cell death–inducing DNA fragmentation factor alpha (DFFA)-like effector (CIDE) family proteins are crucial for various biological processes including unilocular ...